LOCUS       sequence-190236-       17053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human DNA-PKcs/ABCDE>ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17053)
  AUTHORS   Cui X, Yu Y, Gupta S, Cho YM, Lees-Miller SP, Meek K
  TITLE     Autophosphorylation of DNA-dependent protein kinase regulates DNA 
            end processing and may also alter double-strand break repair pathway
            choice.
  JOURNAL   Mol Cell Biol. 2005 Dec;25(24):10842-52.
  PUBMED    16314509
REFERENCE   2  (bases 1 to 17053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-12"; volume: "25(24)"; pages: "10842-52"
COMMENT     Sequence Label: DNA-PKcs ABCDE>A
FEATURES             Location/Qualifiers
     source          1..17053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     584..606
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     598..615
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     599..615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        776..1155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1156..1359
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1310..1330
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    13852..14474
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(14051..14070)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(14498..14518)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14503..14832
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14683..14818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14870..14889)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14871..14889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14903..14919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14903..14919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14916..14938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14927..14943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14951..14957,14958..14975,14976..14981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14996..15017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15134..15151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15305..15893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15385..15404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16064..16855,16856..16924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16687..16706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16925..17029)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
      421 taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc
      481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      601 aaaacgacgg ccagtgccaa gctgatctat acattgaatc aatattggca attagccata
      661 ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
      721 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat
      781 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      841 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      901 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      961 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1021 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1081 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
     1141 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
     1201 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     1261 caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc
     1321 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagaat
     1381 tcagatctgg taccacgcgt atcgataagc ttgcatgcct gcaggtcgac tctagagcgg
     1441 ccgcatggcg ggctccggag ccggtgtgcg ttgctccctg ctgcggctgc aggagacctt
     1501 gtccgctgcg gaccgctgcg gtgctgccct ggccggtcat caactgatcc gcggcctggg
     1561 gcaggaatgc gtcctgagca gcagccccgc ggtgctggca ttacagacat ctttagtttt
     1621 ttccagagat ttcggtttgc ttgtatttgt ccggaagtca ctcaacagta ttgaatttcg
     1681 tgaatgtaga gaagaaatcc taaagttttt atgtattttc ttagaaaaaa tgggccagaa
     1741 gatcgcacct tactctgttg aaattaagaa cacttgtacc agtgtttata caaaagatag
     1801 agctgctaaa tgtaaaattc cagccctgga ccttcttatt aagttacttc agacttttag
     1861 aagttctaga ctcatggatg aatttaaaat tggagaatta tttagtaaat tctatggaga
     1921 acttgcattg aaaaaaaaaa taccagatac agttttagaa aaagtatatg agctcctagg
     1981 attattgggt gaagttcatc ctagtgagat gataaataat gcagaaaacc tgttccgcgc
     2041 ttttctgggt gaacttaaga cccagatgac atcagcagta agagagccca aactacctgt
     2101 tctggcagga tgtctgaagg ggttgtcctc acttctgtgc aacttcacta agtccatgga
     2161 agaagatccc cagacttcaa gggagatttt taattttgta ctaaaggcaa ttcgtcctca
     2221 gattgatctg aagagatatg ctgtgccctc agctggcttg cgcctatttg ccctgcatgc
     2281 atctcagttt agcacctgcc ttctggacaa ctacgtgtct ctatttgaag tcttgttaaa
     2341 gtggtgtgcc cacacaaatg tagaattgaa aaaagctgca ctttcagccc tggaatcctt
     2401 tctgaaacag gtttctaata tggtggcgaa aaatgcagaa atgcataaaa ataaactgca
     2461 gtactttatg gagcagtttt atggaatcat cagaaatgtg gattcgaaca acaaggagtt
     2521 atctattgct atccgtggat atggactttt tgcaggaccg tgcaaggtta taaacgcaaa
     2581 agatgttgac ttcatgtacg ttgagctcat tcagcgctgc aagcagatgt tcctcaccca
     2641 gacagacact ggtgacgacc gtgtttatca gatgccaagc ttcctccagt ctgttgcaag
     2701 cgtcttgctg taccttgaca cagttcctga ggtgtatact ccagttctgg agcacctcgt
     2761 ggtgatgcag atagacagtt tcccacagta cagtccaaaa atgcagctgg tgtgttgcag
     2821 agccatagtg aaggtgttcc tagctttggc agcaaaaggg ccagttctca ggaattgcat
     2881 tagtactgtg gtgcatcagg gtttaatcag aatatgttct aaaccagtgg tccttccaaa
     2941 gggccctgag tctgaatctg aagaccaccg tgcttcaggg gaagtcagaa ctggcaaatg
     3001 gaaggtgccc acatacaaag actacgtgga tctcttcaga catctcctga gctctgacca
     3061 gatgatggat tctattttag cagatgaagc atttttctct gtgaattcct ccagtgaaag
     3121 tctgaatcat ttactttatg atgaatttgt aaaatccgtt ttgaagattg ttgagaaatt
     3181 ggatcttaca cttgaaatac agactgttgg ggaacaagag aatggagatg aggcgcctgg
     3241 tgtttggatg atcccaactt cagatccagc ggctaacttg catccagcta aacctaaaga
     3301 tttttcggct ttcattaacc tggtggaatt ttgcagagag attctccctg agaaacaagc
     3361 agaatttttt gaaccatggg tgtactcatt ttcatatgaa ttaattttgc aatctacaag
     3421 gttgcccctc atcagtggtt tctacaaatt gctttctatt acagtaagaa atgccaagaa
     3481 aataaaatat ttcgagggag ttagtccaaa gagtctgaaa cactctcctg aagacccaga
     3541 aaagtattct tgctttgctt tatttgtgaa atttggcaaa gaggtggcag ttaaaatgaa
     3601 gcagtacaaa gatgaacttt tggcctcttg tttgaccttt cttctgtcct tgccacacaa
     3661 catcattgaa ctcgatgtta gagcctacgt tcctgcactg cagatggctt tcaaactggg
     3721 cctgagctat acccccttgg cagaagtagg cctgaatgct ctagaagaat ggtcaattta
     3781 tattgacaga catgtaatgc agccttatta caaagacatt ctcccctgcc tggatggata
     3841 cctgaagact tcagccttgt cagatgagac caagaataac tgggaagtgt cagctctttc
     3901 tcgggctgcc cagaaaggat ttaataaagt ggtgttaaag catctgaaga agacaaagaa
     3961 cctttcatca aacgaagcaa tatccttaga agaaataaga attagagtag tacaaatgct
     4021 tggatctcta ggaggacaaa taaacaaaaa tcttctgaca gtcacgtcct cagatgagat
     4081 gatgaagagc tatgtggcct gggacagaga gaagcggctg agctttgcag tgccctttag
     4141 agagatgaaa cctgtcattt tcctggatgt gttcctgcct cgagtcacag aattagcgct
     4201 cacagccagt gacagacaaa ctaaggttgc agcctgtgaa cttttacata gcatggttat
     4261 gtttatgttg ggcaaagcca cgcagatgcc agaaggggga cagggagccc cacccatgta
     4321 ccagctctat aagcggacgt ttcctgtgct gcttcgactt gcgtgtgatg ttgatcaggt
     4381 gacaaggcaa ctgtatgagc cactagttat gcagctgatt cactggttca ctaacaacaa
     4441 gaaatttgaa agtcaggata ctgttgcctt actagaagct atattggatg gaattgtgga
     4501 ccctgttgac agtactttaa gagatttttg tggtcggtgt attcgagaat tccttaaatg
     4561 gtccattaag caaataacac cacagcagca ggagaagagt ccagtaaaca ccaaatcgct
     4621 tttcaagcga ctttatagcc ttgcgcttca ccccaatgct ttcaagaggc tgggagcatc
     4681 acttgccttt aataatatct acagggaatt cagggaagaa gagtctctgg tggaacagtt
     4741 tgtgtttgaa gccttggtga tatacatgga gagtctggcc ttagcacatg cagatgagaa
     4801 gtccttaggt acaattcaac agtgttgtga tgccattgat cacctatgcc gcatcattga
     4861 aaagaagcat gtttctttaa ataaagcaaa gaaacgacgt ttgccgcgag gatttccacc
     4921 ttccgcatca ttgtgtttat tggatctggt caagtggctt ttagctcatt gtgggaggcc
     4981 ccagacagaa tgtcgacaca aatccattga actcttttat aaattcgttc ctttattgcc
     5041 aggcaacaga tcccctaatt tgtggctgaa agatgttctc aaggaagaag gtgtctcttt
     5101 tctcatcaac acctttgagg ggggtggctg tggccagccc tcgggcatcc tggcccagcc
     5161 caccctcttg taccttcggg ggccattcag cctgcaggcc acgctatgct ggctggacct
     5221 gctcctggcc gcgttggagt gctacaacac gttcattggc gagagaactg taggagcgct
     5281 ccaggtccta ggtactgaag cccagtcttc acttttgaaa gcagtggctt tcttcttaga
     5341 aagcattgcc atgcatgaca ttatagcagc agaaaagtgc tttggcactg gggcagcagg
     5401 taacagaaca agcccacaag agggagaaag gtacaactac agcaaatgca ccgttgtggt
     5461 ccggattatg gagtttacca cgactctgct aaacacctcc ccggaaggat ggaagctcct
     5521 gaagaaggac ttgtgtaata cacacctgat gagagtcctg gtgcagacgc tgtgtgagcc
     5581 cgcaagcata ggtttcaaca tcggagacgt ccaggttatg gctcatcttc ctgatgtttg
     5641 tgtgaatctg atgaaagctc taaagatgtc cccatacaaa gatatcctag agacccatct
     5701 gagagagaaa ataacagcac agagcattga ggagctttgt gccgtcaact tgtatggccc
     5761 tgacgcgcaa gtggacagga gcaggctggc tgctgttgtg tctgcctgta aacagcttca
     5821 cagagctggg cttctgcata atatattacc gtctcagtcc acagatttgc atcattctgt
     5881 tggcacagaa cttctttccc tggtttataa aggcattgcc cctggagatg agagacagtg
     5941 tctgccttct ctagacctca gttgtaagca gctggccagc ggacttctgg agttagcctt
     6001 tgcttttgga ggactgtgtg agcgccttgt gagtcttctc ctgaacccag cggtgctgtc
     6061 cacggcgtcc ttgggcagct cacagggcag cgtcatccac ttctcccatg gggagtattt
     6121 ctatagcttg ttctcagaaa cgatcaacac ggaattattg aaaaatctgg atcttgctgt
     6181 attggagctc atgcagtctt cagtggataa taccaaaatg gtgagtgccg ttttgaacgg
     6241 catgttagac cagagcttca gggagcgagc aaaccagaaa caccaaggac tgaaacttgc
     6301 gactacaatt ctgcaacact ggaagaagtg tgattcatgg tgggccaaag attcccctct
     6361 cgaaactaaa atggcagtgc tggccttact ggcaaaaatt ttacagattg attcatctgt
     6421 atcttttaat acaagtcatg gttcattccc tgaagtcttt acaacatata ttagtctact
     6481 tgctgacaca aagctggatc tacatttaaa gggccaagct gtcactcttc ttccattctt
     6541 caccagcctc actggaggca gtctggagga acttagacgt gttctggagc agctcatcgt
     6601 tgctcacttc cccatgcagt ccagggaatt tcctccagga actccgcggt tcaataatta
     6661 tgtggactgc atgaaaaagt ttctagatgc attggaatta tctcaaagcc ctatgttgtt
     6721 ggaattgatg acagaagttc tttgtcggga acagcagcat gtcatggaag aattatttca
     6781 atccagtttc aggaggattg ccagaagggg ttcatgtgtc acacaagtag gccttctgga
     6841 aagcgtgtat gaaatgttca ggaaggatga cccccgccta agtttcacac gccagtcctt
     6901 tgtggaccgc tccctcctca ctctgctgtg gcactgtagc ctggatgctt tgagagaatt
     6961 cttcagcaca attgtggtgg atgccattga tgtgttgaag tccaggttta caaagctaaa
     7021 tgaatctacc tttgatactc aaatcaccaa gaagatgggc tactataaga ttctagacgt
     7081 gatgtattct cgccttccca aagatgatgt tcatgctaag gaatcaaaaa ttaatcaagt
     7141 tttccatggc tcgtgtatta cagaaggaaa tgaacttaca aagacattga ttaaattgtg
     7201 ctacgatgca tttacagaga acatggcagg agagaatcag ctgctggaga ggagaagact
     7261 ttaccattgt gcagcataca actgcgccat atctgtcatc tgctgtgtct tcaatgagtt
     7321 aaaattttac caaggttttc tgtttagtga aaaaccagaa aagaacttgc ttatttttga
     7381 aaatctgatc gacctgaagc gccgctataa ttttcctgta gaagttgagg ttcctatgga
     7441 aagaaagaaa aagtacattg aaattaggaa agaagccaga gaagcagcaa atggggattc
     7501 agatggtcct tcctatatgt cttccctgtc atatttggca gacagtaccc tgagtgagga
     7561 aatgagtcaa tttgatttct caaccggagt tcagagctat tcatacagct cccaagaccc
     7621 tagacctgcc actggtcgtt ttcggagacg ggagcagcgg gaccccacgg tgcatgatga
     7681 tgtgctggag ctggagatgg acgagctcaa tcggcatgag tgcatggcgc ccctgacggc
     7741 cctggtcaag cacatgcaca gaagcctggg cccgcctcaa ggagaagagg attcagtgcc
     7801 aagagatctt ccttcttgga tgaaattcct ccatggcaaa ctgggaaatc caatagtacc
     7861 attaaatatc cgtctcttct tagccaagct tgttattaat acagaagagg tctttcgccc
     7921 ttacgcgaag cactggctta gccccttgct gcagctggct gcttctgaaa acaatggagg
     7981 agaaggaatt cactacatgg tggttgagat agtggccact attctttcat ggacaggctt
     8041 ggccactcca acaggggtcc ctaaagatga agtgttagca aatcgattgc ttaatttcct
     8101 aatgaaacat gtctttcatc caaaaagagc tgtgtttaga cacaaccttg aaattataaa
     8161 gacccttgtc gagtgctgga aggattgttt atccatccct tataggttaa tatttgaaaa
     8221 gttttccggt aaagatccta attctaaaga caactcagta gggattcaat tgctaggcat
     8281 cgtgatggcc aatgacctgc ctccctatga cccacagtgt ggcatccaga gtagcgaata
     8341 cttccaggct ttggtgaata atatgtcctt tgtaagatat aaagaagtgt atgccgctgc
     8401 agcagaagtt ctaggactta tacttcgata tgttatagag agaaaaaaca tactggagga
     8461 gtctctgtgt gaactggttg cgaaacaatt gaagcaacat cagaatacta tggaggacaa
     8521 gtttattgtg tgcttgaaca aagtgaccaa gagcttccct cctcttgcag acaggttcat
     8581 gaatgctgtg ttctttctgc tgccaaaatt tcatggagtg ttgaaaacac tctgtctgga
     8641 ggtggtactt tgtcgtgtgg agggaatgac agagctgtac ttccagttaa agagcaagga
     8701 cttcgttcaa gtcatgagac atagagatga tgaaagacaa aaagtatgtt tggacataat
     8761 ttataagatg atgccaaagt taaaaccagt agaactccga gaacttctga accccgttgt
     8821 ggaattcgtt tcccatcctt ctacaacatg tagggaacaa atgtataata ttctcatgtg
     8881 gattcatgat aattacagag atccagaaag tgagacagat aatgactccc aggaaatatt
     8941 taagttggca aaagatgtgc tgattcaagg attgatcgat gagaaccctg gacttcaatt
     9001 aattattcga aatttctgga gccatgaaac taggttacct tcaaatacct tggaccggtt
     9061 gctggcacta aattccttat attctcctaa gatagaagtg cactttttaa gtttagcaac
     9121 aaattttctg ctcgaaatga ccagcatgag cccagattat ccaaacccca tgttcgagca
     9181 tcctctgtca gaatgcgaat ttcaggaata taccattgat tctgattggc gtttccgaag
     9241 tactgttctc actccgatgt ttgtggaggc tcaggccgct cagggcactc tccagacccg
     9301 tgctcaggaa ggggctctct cagctcgctg gccagtggca gggcagataa gggccgctca
     9361 gcagcagcat gacttcacac tggcccagac tgcagatgga agaagctcat ttgattggct
     9421 gaccgggagc agcactgacc cgctggtcga ccacaccagt ccctcatctg actccttgct
     9481 gtttgcccac aagaggagtg aaaggttaca gagagcaccc ttgaagtcag tggggcctga
     9541 ttttgggaaa aaaaggctgg gccttccagg ggacgaggtg gataacaaag tgaaaggtgc
     9601 ggccggccgg acggacctac tacgactgcg cagacggttt atgagggacc aggagaagct
     9661 cagtttgatg tatgccagaa aaggcgttgc tgagcaaaaa cgagagaagg aaatcaagag
     9721 tgagttaaaa atgaagcagg atgcccaggt cgttctgtac agaagctacc ggcacggaga
     9781 ccttcctgac attcagatca agcacagcag cctcatcacc ccgttacagg ccgtggccca
     9841 gagggaccca ataattgcaa aacagctctt tagcagcttg ttttctggaa ttttgaaaga
     9901 gatggataaa tttaagacac tgtctgaaaa aaacaacatc actcaaaagt tgcttcaaga
     9961 cttcaatcgt tttcttaata ccaccttctc tttctttcca ccctttgtct cttgtattca
    10021 ggacattagc tgtcagcacg cagccctgct gagcctcgac ccagcggctg ttagcgctgg
    10081 ttgcctggcc agcctacagc agcccgtggg catccgcctg ctagaggagg ctctgctctg
    10141 cctgctgcct gctgagctgc ccgccaagcg agtccgtggg aaggcccgcc cccctcctga
    10201 tgtcctcaga tgggtggagc ttgctaagct gtatagatca attggagaat acgacgtcct
    10261 ccgtgggatt tttaccagtg agataggaac aaagcaaatc actcagagtg cattattagc
    10321 agaagccaga agtgattatt ctgaagctgc taagcagtat gatgaggctc tcaataaaca
    10381 agactgggta gatggtgagc ccacagaagc cgagaaggat ttttgggaac ttgcatccct
    10441 tgactgttac aaccaccttg ctgagtggaa atcacttgaa tactgttcta cagccagtat
    10501 agacagtgag aaccccccag acctaaataa aatctggagt gaaccatttt atcaggaaac
    10561 atatctacct tacatgatcc gcagcaagct gaagctgctg ctccagggag aggctgacca
    10621 gtccctgctg acatttattg acaaagctat gcacggggag ctccagaagg cgattctaga
    10681 gcttcattac agtcaagagc tgagtctgct ttacctcctg caagatgatg ttgacagagc
    10741 caaatattac attcaaaatg gcattcagag ttttatgcag aattattcta gtattgatgt
    10801 cctcttacac caaagtagac tcaccaaatt gcagtctgta caggctttaa cagaaattca
    10861 ggagttcatc agctttataa gcaaacaagg caatttatca tctcaagttc cccttaagag
    10921 acttctgaac acctgggcaa acagatatcc agatgctaaa atggacccaa tgaacatctg
    10981 ggatgacatc atcacaaatc gatgtttctt tctcagcaaa atagaggaga agcttacccc
    11041 tcttccagaa gataatagta tgaatgtgga tcaagatgga gaccccagtg acaggatgga
    11101 agtgcaagag caggaagaag atatcagctc cctgatcagg agttgcaagt tttccatgaa
    11161 aatgaagatg atagacagtg cccggaagca gaacaatttc tcacttgcta tgaaactact
    11221 gaaggagctg cataaagagt caaaaaccag agacgattgg ctggtgagct gggtgcagag
    11281 ctactgccgc ctgagccact gccggagccg gtcccagggc tgctctgagc aggtgctcac
    11341 tgtgctgaaa acagtctctt tgttggatga gaacaacgtg tcaagctact taagcaaaaa
    11401 tattctggct ttccgtgacc agaacattct cttgggtaca acttacagga tcatagcgaa
    11461 tgctctcagc agtgagccag cctgccttgc tgaaatcgag gaggacaagg ctagaagaat
    11521 cttagagctt tctggatcca gttcagagga ttcagagaag gtgatcgcgg gtctgtacca
    11581 gagagcattc cagcacctct ctgaggctgt gcaggcggct gaggaggagg cccagcctcc
    11641 ctcctggagc tgtgggcctg cagctggggt gattgatgct tacatgacgc tggcagattt
    11701 ctgtgaccaa cagctgcgca aggaggaaga gaatgcatca gttactgatt ctgcagaact
    11761 gcaggcgtat ccagcacttg tggtggagaa aatgttgaaa gctttaaaat taaattccaa
    11821 tgaagccaga ttgaagtttc ctagattact tcagattata gaacggtatc cagaggagac
    11881 tttgagcctc atgacaaaag agatctcttc cgttccctgc tggcagttca tcagctggat
    11941 cagccacatg gtggccttac tggacaaaga ccaagccgtt gctgttcagc actctgtgga
    12001 agaaatcact gataactacc cgcaggctat tgtttatccc ttcatcataa gcagcgaaag
    12061 ctattccttc aaggatactt ctactggtca taagaataag gagtttgtgg caaggattaa
    12121 aagtaagttg gatcaaggag gagtgattca agattttatt aatgccttag atcagctctc
    12181 taatcctgaa ctgctcttta aggattggag caatgatgta agagctgaac tagcaaaaac
    12241 ccctgtaaat aaaaaaaaca ttgaaaaaat gtatgaaaga atgtatgcag ccttgggtga
    12301 cccaaaggct ccaggcctgg gggcctttag aaggaagttt attcagactt ttggaaaaga
    12361 atttgataaa cattttggga aaggaggttc taaactactg agaatgaagc tcagtgactt
    12421 caacgacatt accaacatgc tacttttaaa aatgaacaaa gactcaaagc cccctgggaa
    12481 tctgaaagaa tgttcaccct ggatgagcga cttcaaagtg gagttcctga gaaatgagct
    12541 ggagattccc ggtcagtatg acggtagggg aaagccattg ccagagtacc acgtgcgaat
    12601 cgccgggttt gatgagcggg tgacagtcat ggcgtctctg cgaaggccca agcgcatcat
    12661 catccgtggc catgacgaga gggaacaccc tttcctggtg aagggtggcg aggacctgcg
    12721 gcaggaccag cgcgtggagc agctcttcca ggtcatgaat gggatcctgg cccaagactc
    12781 cgcctgcagc cagagggccc tgcagctgag gacctatagc gttgtgccca tgacctccag
    12841 gttaggatta attgagtggc ttgaaaatac tgttaccttg aaggaccttc ttttgaacac
    12901 catgtcccaa gaggagaagg cggcttacct gagtgatccc agggcaccgc cgtgtgaata
    12961 taaagattgg ctgacaaaaa tgtcaggaaa acatgatgtt ggagcttaca tgctaatgta
    13021 taagggcgct aatcgtactg aaacagtcac gtcttttaga aaacgagaaa gtaaagtgcc
    13081 tgctgatctc ttaaagcggg ccttcgtgag gatgagtaca agccctgagg ctttcctggc
    13141 gctccgctcc cacttcgcca gctctcacgc tctgatatgc atcagccact ggatcctcgg
    13201 gattggagac agacatctga acaactttat ggtggccatg gagactggtg gcgtgatcgg
    13261 gatcgacttt gggcatgcgt ttggatccgc tacacagttt ctgccagtcc ctgagttgat
    13321 gccttttcgg ctaactcgcc agtttatcaa tctgatgtta ccaatgaaag aaacgggcct
    13381 tatgtacagc atcatggtac acgcactccg ggccttccgc tcagaccctg gcctgctcac
    13441 caacaccatg gatgtgtttg tcaaggagcc ctcctttgat tggaaaaatt ttgaacagaa
    13501 aatgctgaaa aaaggagggt catggattca agaaataaat gttgctgaaa aaaattggta
    13561 cccccgacag aaaatatgtt acgctaagag aaagttagca ggtgccaatc cagcagtcat
    13621 tacttgtgat gagctactcc tgggtcatga gaaggcccct gccttcagag actatgtggc
    13681 tgtggcacga ggaagcaaag atcacaacat tcgtgcccaa gaaccagaga gtgggctttc
    13741 agaagagact caagtgaagt gcctgatgga ccaggcaaca gaccccaaca tccttggcag
    13801 aacctgggaa ggatgggagc cctggatgtg aggtctgcgg ccgcggatcc cgggtggcat
    13861 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
    13921 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
    13981 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
    14041 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
    14101 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
    14161 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
    14221 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
    14281 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
    14341 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
    14401 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
    14461 atgcctgttg aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt
    14521 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    14581 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    14641 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    14701 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    14761 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    14821 ggcttttgca aaaagctcct cgaggaactg aaaaaccaga aagttaattc cctatagtga
    14881 gtcgtattaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
    14941 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    15001 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    15061 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    15121 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    15181 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    15241 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    15301 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    15361 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    15421 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    15481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    15541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    15601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    15661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    15721 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    15781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    15841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    15901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    15961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    16021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    16081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    16141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    16201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    16261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    16321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    16381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    16441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    16501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    16561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    16621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    16681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    16741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    16801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    16861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    16921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    16981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    17041 gaaaagtgcc acc
//
