LOCUS       sequence-190237-        9604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral expression of wild-type Becn1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Rocchi A, Yamamoto S, Ting T, Fan Y, Sadleir K, Wang Y, Zhang W, 
            Huang S, Levine B, Vassar R, He C
  TITLE     A Becn1 mutation mediates hyperactive autophagic sequestration of 
            amyloid oligomers and improved cognition in Alzheimer's disease.
  JOURNAL   PLoS Genet. 2017 Aug 14;13(8):e1006962. doi: 
            10.1371/journal.pgen.1006962. eCollection 2017 Aug.
  PUBMED    28806762
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2017-08-14"; volume: "13(8):e1006962. doi"; pages: "
            10.1371/journal.pgen.1006962. eCollection 2017 Aug"
COMMENT     Sequence Label: lenti-HA-mBec1
FEATURES             Location/Qualifiers
     source          1..9604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..293
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     244..264
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     290..314
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             379..405
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1823..2034
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2047..2315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2344..2353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2374..3036
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             3106..3159
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3160..3759
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3160..3179)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3656..3676
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3760..4348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3813..3833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4231..4242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4422..4655
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4727..4848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4764..4783)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4818..4837
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4888..5023
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5056..5072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5056..5072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5069..5091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5080..5096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5104..5110,5111..5128,5129..5134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5149..5170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5287..5304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5458..6046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6217..7008,7009..7077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6840..6859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7078..7182)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7250..7268
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7306..7328)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7428..7447
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7641..7663
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7655..7672
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7656..7672
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7687..7913
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7914..8094
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8141..8266
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8759..8992
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9177..9221
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9488..9604
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
       61 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      121 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      181 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      241 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
      301 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
      361 agagtcgacg ccaccatgta cccatacgat gttccagatt acgctggggg aggcgagggg
      421 tctaaggcgt ccagcagcac catgcaggtg agcttcgtgt gccagcgctg tagccagcct
      481 ctgaaactgg acacgagctt caagatcctg gaccgggtca ccatccagga actcacagct
      541 ccattactta ccacagccca ggcgaaacca ggagagaccc aggaggaaga ggctaatagc
      601 ggtgaagaac ctttcattga aactcgccag gatggtgtct ctcgaagatt catcccccca
      661 gccaggatga tgtctacaga aagtgctaat agcttcactc tgatcgggga ggcatctgat
      721 ggtggcacca tggagaacct cagccggaga ctcaaggtca ctggagacct gtttgacatc
      781 atgtcgggcc agaccgatgt ggatcacccg ctgtgtgagg aatgcacaga cactctttta
      841 gaccagctgg acactcagct caatgtcact gagaatgaat gtcagaacta caaacgctgt
      901 ttggagatcc tggagcagat gaatgaggat gacagtgagc agctgcagag ggagctgaag
      961 gagctggcct tggaggagga gaggctgatc caggagctgg aagatgtgga aaagaaccgc
     1021 aaggtggtgg cagagaacct ggagaaggtc caggctgagg cggagagatt ggaccaggag
     1081 gaagctcagt accagcggga gtatagtgag tttaaaaggc agcagctgga gttggatgac
     1141 gaactcaaga gtgtggagaa ccaggtgcgc tacgcccaga tccagctgga caagctcaag
     1201 aagaccaatg tcttcaatgc caccttccat atctggcaca gcggacagtt tggcacaatc
     1261 aataatttca gactgggtcg cttgcccagc gttccagtgg agtggaatga aatcaatgct
     1321 gcctggggcc agacagtgct gctgctccat gctttggcca ataagatggg tctgaagttt
     1381 cagaggtacc gacttgttcc ctatggaaat cattcctatc tggagtctct gacagacaaa
     1441 tctaaggagt tgccgttata ctgttctggg ggtttgcggt ttttctggga caacaagttt
     1501 gaccatgcaa tggtagcttt tctggactgt gtgcagcagt tcaaagaaga ggtggaaaaa
     1561 ggagagactc gattttgtct tccgtacagg atggacgtgg agaaaggcaa gattgaagac
     1621 actggaggca gtggcggctc ctattccatc aaaacccagt ttaactcgga ggagcagtgg
     1681 acaaaagcgc tcaagttcat gctgaccaat ctcaagtggg gtcttgcctg ggtgtcctca
     1741 cagttctata acaagtgaga attcgaattt aaatcggatc cgcggccgcg aaggatctgc
     1801 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     1861 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     1921 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     1981 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     2041 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     2101 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     2161 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     2221 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     2281 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     2341 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     2401 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     2461 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     2521 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     2581 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     2641 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     2701 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     2761 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     2821 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     2881 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     2941 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     3001 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     3061 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     3121 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     3181 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     3241 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     3301 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     3361 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     3421 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     3481 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     3541 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     3601 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     3661 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     3721 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     3781 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3841 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3901 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3961 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4021 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4081 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4141 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4201 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4261 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt
     4321 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     4381 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     4441 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     4501 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     4561 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4621 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     4681 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     4741 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4801 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     4861 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4921 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4981 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     5041 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5101 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5161 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5221 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5641 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5881 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5941 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6061 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6421 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6481 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6541 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6601 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6661 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6721 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6781 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6841 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6901 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6961 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7021 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7081 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7141 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7201 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     7261 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     7321 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     7381 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     7441 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     7501 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     7561 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     7621 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     7681 gacgcgtgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7741 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7801 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7861 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8101 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8161 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8221 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8281 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8341 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8401 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8461 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8521 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8581 aagtaagacc accgcacagc aagcggccac tgatcttcag acctggagga ggagatatga
     8641 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8701 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8761 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8821 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8881 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8941 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     9001 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     9061 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9121 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9181 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9241 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9301 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9361 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     9421 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     9481 ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9541 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa
     9601 tttt
//
