LOCUS       sequence-190247-       17053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human DNA-PKcs PQR>ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17053)
  AUTHORS   Cui X, Yu Y, Gupta S, Cho YM, Lees-Miller SP, Meek K
  TITLE     Autophosphorylation of DNA-dependent protein kinase regulates DNA 
            end processing and may also alter double-strand break repair pathway
            choice.
  JOURNAL   Mol Cell Biol. 2005 Dec;25(24):10842-52.
  PUBMED    16314509
REFERENCE   2  (bases 1 to 17053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-12"; volume: "25(24)"; pages: "10842-52"
COMMENT     Sequence Label: DNA-PKcs PQR>ala
FEATURES             Location/Qualifiers
     source          1..17053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     582..604
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     596..613
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     597..613
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        774..1153
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1154..1357
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1308..1328
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    13850..14472
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(14049..14068)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(14496..14516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14501..14830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14681..14816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14868..14887)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14869..14887)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14901..14917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14901..14917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14914..14936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14925..14941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14949..14955,14956..14973,14974..14979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14994..15015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15132..15149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15303..15891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15383..15402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16062..16853,16854..16922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16685..16704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16923..17027)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
      481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      541 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      601 aacgacggcc agtgccaagc tgatctatac attgaatcaa tattggcaat tagccatatt
      661 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
      721 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
      781 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      841 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      901 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      961 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     1021 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     1081 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     1141 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     1201 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1261 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     1321 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagaattc
     1381 agatctggta ccacgcgtat cgataagctt gcatgcctgc aggtcgactc tagagcggcc
     1441 gcatggcggg ctccggagcc ggtgtgcgtt gctccctgct gcggctgcag gagaccttgt
     1501 ccgctgcgga ccgctgcggt gctgccctgg ccggtcatca actgatccgc ggcctggggc
     1561 aggaatgcgt cctgagcagc agccccgcgg tgctggcatt acagacatct ttagtttttt
     1621 ccagagattt cggtttgctt gtatttgtcc ggaagtcact caacagtatt gaatttcgtg
     1681 aatgtagaga agaaatccta aagtttttat gtattttctt agaaaaaatg ggccagaaga
     1741 tcgcacctta ctctgttgaa attaagaaca cttgtaccag tgtttataca aaagatagag
     1801 ctgctaaatg taaaattcca gccctggacc ttcttattaa gttacttcag acttttagaa
     1861 gttctagact catggatgaa tttaaaattg gagaattatt tagtaaattc tatggagaac
     1921 ttgcattgaa aaaaaaaata ccagatacag ttttagaaaa agtatatgag ctcctaggat
     1981 tattgggtga agttcatcct agtgagatga taaataatgc agaaaacctg ttccgcgctt
     2041 ttctgggtga acttaagacc cagatgacat cagcagtaag agagcccaaa ctacctgttc
     2101 tggcaggatg tctgaagggg ttgtcctcac ttctgtgcaa cttcactaag tccatggaag
     2161 aagatcccca gacttcaagg gagattttta attttgtact aaaggcaatt cgtcctcaga
     2221 ttgatctgaa gagatatgct gtgccctcag ctggcttgcg cctatttgcc ctgcatgcat
     2281 ctcagtttag cacctgcctt ctggacaact acgtgtctct atttgaagtc ttgttaaagt
     2341 ggtgtgccca cacaaatgta gaattgaaaa aagctgcact ttcagccctg gaatcctttc
     2401 tgaaacaggt ttctaatatg gtggcgaaaa atgcagaaat gcataaaaat aaactgcagt
     2461 actttatgga gcagttttat ggaatcatca gaaatgtgga ttcgaacaac aaggagttat
     2521 ctattgctat ccgtggatat ggactttttg caggaccgtg caaggttata aacgcaaaag
     2581 atgttgactt catgtacgtt gagctcattc agcgctgcaa gcagatgttc ctcacccaga
     2641 cagacactgg tgacgaccgt gtttatcaga tgccaagctt cctccagtct gttgcaagcg
     2701 tcttgctgta ccttgacaca gttcctgagg tgtatactcc agttctggag cacctcgtgg
     2761 tgatgcagat agacagtttc ccacagtaca gtccaaaaat gcagctggtg tgttgcagag
     2821 ccatagtgaa ggtgttccta gctttggcag caaaagggcc agttctcagg aattgcatta
     2881 gtactgtggt gcatcagggt ttaatcagaa tatgttctaa accagtggtc cttccaaagg
     2941 gccctgagtc tgaatctgaa gaccaccgtg cttcagggga agtcagaact ggcaaatgga
     3001 aggtgcccac atacaaagac tacgtggatc tcttcagaca tctcctgagc tctgaccaga
     3061 tgatggattc tattttagca gatgaagcat ttttctctgt gaattcctcc agtgaaagtc
     3121 tgaatcattt actttatgat gaatttgtaa aatccgtttt gaagattgtt gagaaattgg
     3181 atcttacact tgaaatacag actgttgggg aacaagagaa tggagatgag gcgcctggtg
     3241 tttggatgat cccaacttca gatccagcgg ctaacttgca tccagctaaa cctaaagatt
     3301 tttcggcttt cattaacctg gtggaatttt gcagagagat tctccctgag aaacaagcag
     3361 aattttttga accatgggtg tactcatttt catatgaatt aattttgcaa tctacaaggt
     3421 tgcccctcat cagtggtttc tacaaattgc tttctattac agtaagaaat gccaagaaaa
     3481 taaaatattt cgagggagtt agtccaaaga gtctgaaaca ctctcctgaa gacccagaaa
     3541 agtattcttg ctttgcttta tttgtgaaat ttggcaaaga ggtggcagtt aaaatgaagc
     3601 agtacaaaga tgaacttttg gcctcttgtt tgacctttct tctgtccttg ccacacaaca
     3661 tcattgaact cgatgttaga gcctacgttc ctgcactgca gatggctttc aaactgggcc
     3721 tgagctatac ccccttggca gaagtaggcc tgaatgctct agaagaatgg tcaatttata
     3781 ttgacagaca tgtaatgcag ccttattaca aagacattct cccctgcctg gatggatacc
     3841 tgaagacttc agccttgtca gatgagacca agaataactg ggaagtgtca gctctttctc
     3901 gggctgccca gaaaggattt aataaagtgg tgttaaagca tctgaagaag acaaagaacc
     3961 tttcatcaaa cgaagcaata tccttagaag aaataagaat tagagtagta caaatgcttg
     4021 gatctctagg aggacaaata aacaaaaatc ttctgacagt cacgtcctca gatgagatga
     4081 tgaagagcta tgtggcctgg gacagagaga agcggctgag ctttgcagtg ccctttagag
     4141 agatgaaacc tgtcattttc ctggatgtgt tcctgcctcg agtcacagaa ttagcgctca
     4201 cagccagtga cagacaaact aaggttgcag cctgtgaact tttacatagc atggttatgt
     4261 ttatgttggg caaagccacg cagatgccag aagggggaca gggagcccca cccatgtacc
     4321 agctctataa gcggacgttt cctgtgctgc ttcgacttgc gtgtgatgtt gatcaggtga
     4381 caaggcaact gtatgagcca ctagttatgc agctgattca ctggttcact aacaacaaga
     4441 aatttgaaag tcaggatact gttgccttac tagaagctat attggatgga attgtggacc
     4501 ctgttgacag tactttaaga gatttttgtg gtcggtgtat tcgagaattc cttaaatggt
     4561 ccattaagca aataacacca cagcagcagg agaagagtcc agtaaacacc aaatcgcttt
     4621 tcaagcgact ttatagcctt gcgcttcacc ccaatgcttt caagaggctg ggagcatcac
     4681 ttgcctttaa taatatctac agggaattca gggaagaaga gtctctggtg gaacagtttg
     4741 tgtttgaagc cttggtgata tacatggaga gtctggcctt agcacatgca gatgagaagt
     4801 ccttaggtac aattcaacag tgttgtgatg ccattgatca cctatgccgc atcattgaaa
     4861 agaagcatgt ttctttaaat aaagcaaaga aacgacgttt gccgcgagga tttccacctt
     4921 ccgcatcatt gtgtttattg gatctggtca agtggctttt agctcattgt gggaggcccc
     4981 agacagaatg tcgacacaaa tccattgaac tcttttataa attcgttcct ttattgccag
     5041 gcaacagatc ccctaatttg tggctgaaag atgttctcaa ggaagaaggt gtctcttttc
     5101 tcatcaacac ctttgagggg ggtggctgtg gccagccctc gggcatcctg gcccagccca
     5161 ccctcttgta ccttcggggg ccattcagcc tgcaggccac gctatgctgg ctggacctgc
     5221 tcctggccgc gttggagtgc tacaacacgt tcattggcga gagaactgta ggagcgctcc
     5281 aggtcctagg tactgaagcc cagtcttcac ttttgaaagc agtggctttc ttcttagaaa
     5341 gcattgccat gcatgacatt atagcagcag aaaagtgctt tggcactggg gcagcaggta
     5401 acagaacaag cccacaagag ggagaaaggt acaactacag caaatgcacc gttgtggtcc
     5461 ggattatgga gtttaccacg actctgctaa acacctcccc ggaaggatgg aagctcctga
     5521 agaaggactt gtgtaataca cacctgatga gagtcctggt gcagacgctg tgtgagcccg
     5581 caagcatagg tttcaacatc ggagacgtcc aggttatggc tcatcttcct gatgtttgtg
     5641 tgaatctgat gaaagctcta aagatgtccc catacaaaga tatcctagag acccatctga
     5701 gagagaaaat aacagcacag agcattgagg agctttgtgc cgtcaacttg tatggccctg
     5761 acgcgcaagt ggacaggagc aggctggctg ctgttgtgtc tgcctgtaaa cagcttcaca
     5821 gagctgggct tctgcataat atattaccgt ctcagtccac agatttgcat cattctgttg
     5881 gcacagaact tctttccctg gtttataaag gcattgcccc tggagatgag agacagtgtc
     5941 tgccttctct agacctcagt tgtaagcagc tggccagcgg acttctggag ttagcctttg
     6001 cttttggagg actgtgtgag cgccttgtga gtcttctcct gaacccagcg gtgctgtcca
     6061 cggcgtcctt gggcagctca cagggcagcg tcatccactt ctcccatggg gagtatttct
     6121 atagcttgtt ctcagaaacg atcaacacgg aattattgaa aaatctggat cttgctgtat
     6181 tggagctcat gcagtcttca gtggataata ccaaaatggt gagtgccgtt ttgaacggca
     6241 tgttagacca gagcttcagg gagcgagcaa accagaaaca ccaaggactg aaacttgcga
     6301 ctacaattct gcaacactgg aagaagtgtg attcatggtg ggccaaagat tcccctctcg
     6361 aaactaaaat ggcagtgctg gccttactgg caaaaatttt acagattgat tcatctgtat
     6421 cttttaatac aagtcatggt tcattccctg aagtctttac aacatatatt agtctacttg
     6481 ctgacacaaa gctggatcta catttaaagg gccaagctgt cactcttctt ccattcttca
     6541 ccagcctcac tggaggcagt ctggaggaac ttagacgtgt tctggagcag ctcatcgttg
     6601 ctcacttccc catgcagtcc agggaatttc ctccaggaac tccgcggttc aataattatg
     6661 tggactgcat gaaaaagttt ctagatgcat tggaattatc tcaaagccct atgttgttgg
     6721 aattgatgac agaagttctt tgtcgggaac agcagcatgt catggaagaa ttatttcaat
     6781 ccagtttcag gaggattgcc agaaggggtt catgtgtcac acaagtaggc cttctggaaa
     6841 gcgtgtatga aatgttcagg aaggatgacc cccgcctaag tttcacacgc cagtcctttg
     6901 tggaccgctc cctcctcact ctgctgtggc actgtagcct ggatgctttg agagaattct
     6961 tcagcacaat tgtggtggat gccattgatg tgttgaagtc caggtttaca aagctaaatg
     7021 aatctacctt tgatactcaa atcaccaaga agatgggcta ctataagatt ctagacgtga
     7081 tgtattctcg ccttcccaaa gatgatgttc atgctaagga atcaaaaatt aatcaagttt
     7141 tccatggctc gtgtattaca gaaggaaatg aacttacaaa gacattgatt aaattgtgct
     7201 acgatgcatt tacagagaac atggcaggag agaatcagct gctggagagg agaagacttt
     7261 accattgtgc agcatacaac tgcgccatat ctgtcatctg ctgtgtcttc aatgagttaa
     7321 aattttacca aggttttctg tttagtgaaa aaccagaaaa gaacttgctt atttttgaaa
     7381 atctgatcga cctgaagcgc cgctataatt ttcctgtaga agttgaggtt cctatggaaa
     7441 gaaagaaaaa gtacattgaa attaggaaag aagccagaga agcagcaaat ggggattcag
     7501 atggtccggc ctatatgtct tccctggcat atttggcaga cagtaccctg agtgaagaaa
     7561 tggctcaatt tgatttctca accggagttc agagctatgc atacagcgcc caagacccta
     7621 ggcctgccac tggtcgtttt cggagacggg agcagcggga ccccacggtg catgatgatg
     7681 tgctggagct ggagatggac gagctcaatc ggcatgagtg catggcgccc ctgacggccc
     7741 tggtcaagca catgcacaga agcctgggcc cgcctcaagg agaagaggat tcagtgccaa
     7801 gagatcttcc ttcttggatg aaattcctcc atggcaaact gggaaatcca atagtaccat
     7861 taaatatccg tctcttctta gccaagcttg ttattaatac agaagaggtc tttcgccctt
     7921 acgcgaagca ctggcttagc cccttgctgc agctggctgc ttctgaaaac aatggaggag
     7981 aaggaattca ctacatggtg gttgagatag tggccactat tctttcatgg acaggcttgg
     8041 ccactccaac aggggtccct aaagatgaag tgttagcaaa tcgattgctt aatttcctaa
     8101 tgaaacatgt ctttcatcca aaaagagctg tgtttagaca caaccttgaa attataaaga
     8161 cccttgtcga gtgctggaag gattgtttat ccatccctta taggttaata tttgaaaagt
     8221 tttccggtaa agatcctaat tctaaagaca actcagtagg gattcaattg ctaggcatcg
     8281 tgatggccaa tgacctgcct ccctatgacc cacagtgtgg catccagagt agcgaatact
     8341 tccaggcttt ggtgaataat atgtcctttg taagatataa agaagtgtat gccgctgcag
     8401 cagaagttct aggacttata cttcgatatg ttatagagag aaaaaacata ctggaggagt
     8461 ctctgtgtga actggttgcg aaacaattga agcaacatca gaatactatg gaggacaagt
     8521 ttattgtgtg cttgaacaaa gtgaccaaga gcttccctcc tcttgcagac aggttcatga
     8581 atgctgtgtt ctttctgctg ccaaaatttc atggagtgtt gaaaacactc tgtctggagg
     8641 tggtactttg tcgtgtggag ggaatgacag agctgtactt ccagttaaag agcaaggact
     8701 tcgttcaagt catgagacat agagatgatg aaagacaaaa agtatgtttg gacataattt
     8761 ataagatgat gccaaagtta aaaccagtag aactccgaga acttctgaac cccgttgtgg
     8821 aattcgtttc ccatccttct acaacatgta gggaacaaat gtataatatt ctcatgtgga
     8881 ttcatgataa ttacagagat ccagaaagtg agacagataa tgactcccag gaaatattta
     8941 agttggcaaa agatgtgctg attcaaggat tgatcgatga gaaccctgga cttcaattaa
     9001 ttattcgaaa tttctggagc catgaaacta ggttaccttc aaataccttg gaccggttgc
     9061 tggcactaaa ttccttatat tctcctaaga tagaagtgca ctttttaagt ttagcaacaa
     9121 attttctgct cgaaatgacc agcatgagcc cagattatcc aaaccccatg ttcgagcatc
     9181 ctctgtcaga atgcgaattt caggaatata ccattgattc tgattggcgt ttccgaagta
     9241 ctgttctcac tccgatgttt gtggagaccc aggcctccca gggcactctc caggcccgta
     9301 cccaggaagg gtccctctca gctcgctggc cagtggcagg gcagataagg gccacccagc
     9361 agcagcatga cttcacactg acacagactg cagatggaag aagctcattt gattggctga
     9421 ccgggagcag cactgacccg ctggtcgacc acaccagtcc ctcatctgac tccttgctgt
     9481 ttgcccacaa gaggagtgaa aggttacaga gagcaccctt gaagtcagtg gggcctgatt
     9541 ttgggaaaaa aaggctgggc cttccagggg acgaggtgga taacaaagtg aaaggtgcgg
     9601 ccggccggac ggacctacta cgactgcgca gacggtttat gagggaccag gagaagctca
     9661 gtttgatgta tgccagaaaa ggcgttgctg agcaaaaacg agagaaggaa atcaagagtg
     9721 agttaaaaat gaagcaggat gcccaggtcg ttctgtacag aagctaccgg cacggagacc
     9781 ttcctgacat tcagatcaag cacagcagcc tcatcacccc gttacaggcc gtggcccaga
     9841 gggacccaat aattgcaaaa cagctcttta gcagcttgtt ttctggaatt ttgaaagaga
     9901 tggataaatt taagacactg tctgaaaaaa acaacatcac tcaaaagttg cttcaagact
     9961 tcaatcgttt tcttaatacc accttctctt tctttccacc ctttgtctct tgtattcagg
    10021 acattagctg tcagcacgca gccctgctga gcctcgaccc agcggctgtt agcgctggtt
    10081 gcctggccag cctacagcag cccgtgggca tccgcctgct agaggaggct ctgctctgcc
    10141 tgctgcctgc tgagctgccc gccaagcgag tccgtgggaa ggcccgcccc cctcctgatg
    10201 tcctcagatg ggtggagctt gctaagctgt atagatcaat tggagaatac gacgtcctcc
    10261 gtgggatttt taccagtgag ataggaacaa agcaaatcac tcagagtgca ttattagcag
    10321 aagccagaag tgattattct gaagctgcta agcagtatga tgaggctctc aataaacaag
    10381 actgggtaga tggtgagccc acagaagccg agaaggattt ttgggaactt gcatcccttg
    10441 actgttacaa ccaccttgct gagtggaaat cacttgaata ctgttctaca gccagtatag
    10501 acagtgagaa ccccccagac ctaaataaaa tctggagtga accattttat caggaaacat
    10561 atctacctta catgatccgc agcaagctga agctgctgct ccagggagag gctgaccagt
    10621 ccctgctgac atttattgac aaagctatgc acggggagct ccagaaggcg attctagagc
    10681 ttcattacag tcaagagctg agtctgcttt acctcctgca agatgatgtt gacagagcca
    10741 aatattacat tcaaaatggc attcagagtt ttatgcagaa ttattctagt attgatgtcc
    10801 tcttacacca aagtagactc accaaattgc agtctgtaca ggctttaaca gaaattcagg
    10861 agttcatcag ctttataagc aaacaaggca atttatcatc tcaagttccc cttaagagac
    10921 ttctgaacac ctgggcaaac agatatccag atgctaaaat ggacccaatg aacatctggg
    10981 atgacatcat cacaaatcga tgtttctttc tcagcaaaat agaggagaag cttacccctc
    11041 ttccagaaga taatagtatg aatgtggatc aagatggaga ccccagtgac aggatggaag
    11101 tgcaagagca ggaagaagat atcagctccc tgatcaggag ttgcaagttt tccatgaaaa
    11161 tgaagatgat agacagtgcc cggaagcaga acaatttctc acttgctatg aaactactga
    11221 aggagctgca taaagagtca aaaaccagag acgattggct ggtgagctgg gtgcagagct
    11281 actgccgcct gagccactgc cggagccggt cccagggctg ctctgagcag gtgctcactg
    11341 tgctgaaaac agtctctttg ttggatgaga acaacgtgtc aagctactta agcaaaaata
    11401 ttctggcttt ccgtgaccag aacattctct tgggtacaac ttacaggatc atagcgaatg
    11461 ctctcagcag tgagccagcc tgccttgctg aaatcgagga ggacaaggct agaagaatct
    11521 tagagctttc tggatccagt tcagaggatt cagagaaggt gatcgcgggt ctgtaccaga
    11581 gagcattcca gcacctctct gaggctgtgc aggcggctga ggaggaggcc cagcctccct
    11641 cctggagctg tgggcctgca gctggggtga ttgatgctta catgacgctg gcagatttct
    11701 gtgaccaaca gctgcgcaag gaggaagaga atgcatcagt tactgattct gcagaactgc
    11761 aggcgtatcc agcacttgtg gtggagaaaa tgttgaaagc tttaaaatta aattccaatg
    11821 aagccagatt gaagtttcct agattacttc agattataga acggtatcca gaggagactt
    11881 tgagcctcat gacaaaagag atctcttccg ttccctgctg gcagttcatc agctggatca
    11941 gccacatggt ggccttactg gacaaagacc aagccgttgc tgttcagcac tctgtggaag
    12001 aaatcactga taactacccg caggctattg tttatccctt catcataagc agcgaaagct
    12061 attccttcaa ggatacttct actggtcata agaataagga gtttgtggca aggattaaaa
    12121 gtaagttgga tcaaggagga gtgattcaag attttattaa tgccttagat cagctctcta
    12181 atcctgaact gctctttaag gattggagca atgatgtaag agctgaacta gcaaaaaccc
    12241 ctgtaaataa aaaaaacatt gaaaaaatgt atgaaagaat gtatgcagcc ttgggtgacc
    12301 caaaggctcc aggcctgggg gcctttagaa ggaagtttat tcagactttt ggaaaagaat
    12361 ttgataaaca ttttgggaaa ggaggttcta aactactgag aatgaagctc agtgacttca
    12421 acgacattac caacatgcta cttttaaaaa tgaacaaaga ctcaaagccc cctgggaatc
    12481 tgaaagaatg ttcaccctgg atgagcgact tcaaagtgga gttcctgaga aatgagctgg
    12541 agattcccgg tcagtatgac ggtaggggaa agccattgcc agagtaccac gtgcgaatcg
    12601 ccgggtttga tgagcgggtg acagtcatgg cgtctctgcg aaggcccaag cgcatcatca
    12661 tccgtggcca tgacgagagg gaacaccctt tcctggtgaa gggtggcgag gacctgcggc
    12721 aggaccagcg cgtggagcag ctcttccagg tcatgaatgg gatcctggcc caagactccg
    12781 cctgcagcca gagggccctg cagctgagga cctatagcgt tgtgcccatg acctccaggt
    12841 taggattaat tgagtggctt gaaaatactg ttaccttgaa ggaccttctt ttgaacacca
    12901 tgtcccaaga ggagaaggcg gcttacctga gtgatcccag ggcaccgccg tgtgaatata
    12961 aagattggct gacaaaaatg tcaggaaaac atgatgttgg agcttacatg ctaatgtata
    13021 agggcgctaa tcgtactgaa acagtcacgt cttttagaaa acgagaaagt aaagtgcctg
    13081 ctgatctctt aaagcgggcc ttcgtgagga tgagtacaag ccctgaggct ttcctggcgc
    13141 tccgctccca cttcgccagc tctcacgctc tgatatgcat cagccactgg atcctcggga
    13201 ttggagacag acatctgaac aactttatgg tggccatgga gactggtggc gtgatcggga
    13261 tcgactttgg gcatgcgttt ggatccgcta cacagtttct gccagtccct gagttgatgc
    13321 cttttcggct aactcgccag tttatcaatc tgatgttacc aatgaaagaa acgggcctta
    13381 tgtacagcat catggtacac gcactccggg ccttccgctc agaccctggc ctgctcacca
    13441 acaccatgga tgtgtttgtc aaggagccct cctttgattg gaaaaatttt gaacagaaaa
    13501 tgctgaaaaa aggagggtca tggattcaag aaataaatgt tgctgaaaaa aattggtacc
    13561 cccgacagaa aatatgttac gctaagagaa agttagcagg tgccaatcca gcagtcatta
    13621 cttgtgatga gctactcctg ggtcatgaga aggcccctgc cttcagagac tatgtggctg
    13681 tggcacgagg aagcaaagat cacaacattc gtgcccaaga accagagagt gggctttcag
    13741 aagagactca agtgaagtgc ctgatggacc aggcaacaga ccccaacatc cttggcagaa
    13801 cctgggaagg atgggagccc tggatgtgag gtctgcggcc gcggatcccg ggtggcatcc
    13861 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
    13921 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
    13981 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
    14041 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
    14101 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca
    14161 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat
    14221 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa
    14281 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata
    14341 actataccag caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt
    14401 gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat
    14461 gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt tagggtgtgg
    14521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    14581 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    14641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    14701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    14761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    14821 cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc tatagtgagt
    14881 cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    14941 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    15001 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    15061 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    15121 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    15181 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    15241 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    15301 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    15361 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    15421 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    15481 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    15541 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    15601 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    15661 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    15721 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    15781 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    15841 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    15901 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    15961 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    16021 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    16081 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    16141 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    16201 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    16261 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    16321 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    16381 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    16441 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    16501 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    16561 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    16621 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    16681 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    16741 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    16801 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    16861 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    16921 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    16981 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    17041 aaagtgccac ctg
//
