LOCUS       sequence-190261-       10724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter to monitor translation of unstructured mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Han K, Jaimovich A, Dey G, Ruggero D, Meyuhas O, Sonenberg N, Meyer 
            T
  TITLE     Parallel measurement of dynamic changes in translation rates in 
            single cells.
  JOURNAL   Nat Methods. 2014 Jan;11(1):86-93. doi: 10.1038/nmeth.2729. Epub 
            2013 Nov 10.
  PUBMED    24213167
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2729"; journalName: "Nat Methods"; date: "2014-01"; 
            volume: "11"; issue: "1"; pages: "86-93"
COMMENT     Sequence Label: UnReg-H2B-YFP-DD
FEATURES             Location/Qualifiers
     source          1..10724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1725..1749
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        1770..2149
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2152..2427
                     /label=chicken beta-actin promoter
     intron          2428..3436
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3360..3382
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3444..3463
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3507..3884
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(3891..3893,3894..4604)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3933..3954)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4194..4213)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4541..4562
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4611..5087
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     protein_bind    complement(5102..5126)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5157..5745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5210..5230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5628..5639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5759..6258
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5794..5817)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6191..6210
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6279..6878
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6279..6298)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6775..6795
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7016..7249
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7321..7442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7358..7377)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7412..7431
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7482..7617
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7637..7656)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7638..7656)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7666..7683)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7666..7682)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7675..7697)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7824..8279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7911..7930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8121..8142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8305..8409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8410..8478,8479..9270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8628..8647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9441..10029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9930..9949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10183..10200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10317..10338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10353..10358,10359..10376,10377..10383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10391..10407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10396..10418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10415..10431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10415..10431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10450..10470
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10452..10470
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10498..10724
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
     1741 aagcaggctt taaaggaacc aattcagtcg acattgatta ttgactagtt attaatagta
     1801 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     1861 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     1921 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt
     1981 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2041 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2101 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg gtcgaggtga
     2161 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     2221 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca
     2281 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
     2341 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     2401 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg
     2461 ctccgcgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     2521 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctc
     2581 gtttcttttc tgtggctgcg tgaaagcctt aaagggctcc gggagggccc tttgtgcggg
     2641 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc
     2701 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg
     2761 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cgggggggct gcgaggggaa
     2821 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt
     2881 cgggctgtaa cccccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     2941 gtgcggggct ccgtgcgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     3001 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     3061 gcggcggccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     3121 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctggc ggagccgaaa
     3181 tctgggaggc gccgccgcac cccctctagc gggcgcgggc gaagcggtgc ggcgccggca
     3241 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc
     3301 tccagcctcg gggctgccgc agggggacgg ctgccttcgg gggggacggg gcagggcggg
     3361 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     3421 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     3481 caaagaattg ctcgagacct aggaccatgc cagagccagc gaagtctgct cccgccccga
     3541 aaaagggctc caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc
     3601 gcagccgcaa ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg
     3661 acaccggcat ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg
     3721 agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct
     3781 ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg
     3841 tgtccgaggg tactaaggcc atcaccaagt acaccagcgc taaggatcca gtgagcaagg
     3901 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3961 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4021 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4081 tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     4141 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4201 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4261 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4321 actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     4381 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     4441 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     4501 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4561 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggtcgag atcagtctga
     4621 ttgcggcgtt agcggtagat cacgttatcg gcatggaaac cgtcatgccg tggaacctgc
     4681 ctgccgatct cgcctggttt aaacgcaaca ccttaaataa acccgtgatt atgggccgcc
     4741 atacctggga atcaatcggt cgtccgttgc caggacgcaa aaatattatc ctcagcagtc
     4801 aaccgagtac ggacgatcgc gtaacgtggg tgaagtcggt ggatgaagcc atcgcggcgt
     4861 gtggtgacgt accagaaatc atggttattg gcggcggtcg cgtttatgaa cagttcttgc
     4921 caaaagcgca aaaactgtat ctgacgcata tcgacgcaga agtggaaggc gacacccatt
     4981 tcccggatta cgagccggat gactgggaat cggtattcag cgaattccac gatgctgatg
     5041 cgcagaactc tcacagctat tgctttgaga ttctggagcg gcgatgagtc gagatatcta
     5101 gacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgacaatc
     5161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5521 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5581 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctaccgg
     5761 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     5821 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     5881 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     5941 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     6001 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     6061 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     6121 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     6181 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     6241 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
     6301 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     6361 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6421 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6481 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6541 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6601 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6661 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6721 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6781 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6841 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     6901 gcccgaccga aaggagcgca cgaccccatg catctcgagg gcccggtacc tttaagacca
     6961 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7021 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     7081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7141 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7261 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7441 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     7501 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7561 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7621 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     7681 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     7741 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7801 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7861 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7921 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7981 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8041 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8101 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8161 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     8221 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     8281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8401 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8461 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8521 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8581 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8641 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8701 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8761 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8821 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8881 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8941 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9001 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9061 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9121 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10201 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    10321 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    10381 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
    10441 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
    10501 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
    10561 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
    10621 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
    10681 attgcagaga tattgtattt aagtgcctag ctcgatacat aaac
//
