LOCUS       sequence-190274-        9713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral
            vector. *Please see comments section below about the number of 
            verified repeats..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Jain A, Vale RD
  TITLE     RNA phase transitions in repeat expansion disorders.
  JOURNAL   Nature. 2017 Jun 8;546(7657):243-247. doi: 10.1038/nature22386. Epub
            2017 May 31.
  PUBMED    28562589
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22386"; journalName: "Nature"; date: "2017-06-8- 8"; 
            volume: "546"; issue: "7657"; pages: "243-247"
COMMENT     Sequence Label: pHR-Tre3G-29xGGGGCC-12xMS2
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        116..134
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        155..173
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        225..243
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        264..282
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        334..352
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        373..391
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        443..461
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        482..500
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        552..570
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        591..609
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        661..679
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        700..718
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_feature    824..1412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(877..897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1295..1306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1415..1431)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1416..1432)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1522..1755
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1780..1798)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1781..1798)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2088..2107)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2321..2340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2440..2462
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2500..2518)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2586..2690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2691..2759,2760..3551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2909..2928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3722..4310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4211..4230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4464..4481
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4551..4571)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4556..4885
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4736..4871
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6019..6084
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6214..6234
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6659..6793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6696..6715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6750..6769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6961..7594
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7641..7766
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8263..8496
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8681..8725
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9010..9127
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9189..9538
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    9197..9215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9233..9251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9269..9287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9305..9323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9341..9359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9377..9395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9413..9431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9477..9501
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggggccgggg ccggggccgg ggccggggcc ggggccgggg ccggggcctc tcgagtctag
       61 agggccgcaa gcttggtacc gagctcggat cctaaggtac ctaattgcct agaaaacatg
      121 aggatcaccc atgtctgcag gtcgactcta gaaaacatga ggatcaccca tgtctgcagt
      181 attcccgggt tcattagatc ctaaggtacc taattgccta gaaaacatga ggatcaccca
      241 tgtctgcagg tcgactctag aaaacatgag gatcacccat gtctgcagta ttcccgggtt
      301 cattagatcc taaggtacct aattgcctag aaaacatgag gatcacccat gtctgcaggt
      361 cgactctaga aaacatgagg atcacccatg tctgcagtat tcccgggttc attagatcct
      421 aaggtaccta attgcctaga aaacatgagg atcacccatg tctgcaggtc gactctagaa
      481 aacatgagga tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa
      541 ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actctagaaa acatgaggat
      601 cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
      661 acatgaggat cacccatgtc tgcaggtcga ctccagaaaa catgaggatc acccatgtct
      721 gcagtattcc cgggttcatt agatctgcgc gcgatcgata tcagcgcttt aaatttgcgc
      781 atgctagtgt aaggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
      841 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
      901 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
      961 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     1021 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     1081 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     1141 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     1201 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     1261 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     1321 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     1381 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagggaatta
     1441 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     1501 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     1561 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     1621 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     1681 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     1741 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     1801 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
     1861 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
     1921 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
     1981 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
     2041 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     2101 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     2161 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     2221 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     2281 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
     2341 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2401 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2461 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     2521 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     2581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     4561 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4621 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4681 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4741 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4801 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4861 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     4921 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     4981 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     5041 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     5101 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     5161 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     5221 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     5281 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     5341 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     5401 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     5461 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     5521 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     5581 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     5641 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     5701 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     5761 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     5821 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     5881 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
     5941 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     6001 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     6061 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     6121 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     6181 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     6241 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     6301 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     6361 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     6421 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     6481 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     6541 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     6601 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     6661 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6721 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6781 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     6841 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     6901 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     6961 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     7021 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     7081 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     7141 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     7201 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     7261 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     7321 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     7381 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     7441 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7501 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7561 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7621 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7681 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7741 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7801 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7861 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     7921 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     7981 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8041 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8101 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     8161 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8221 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8281 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8341 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8401 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     8461 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     8521 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     8581 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     8641 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     8701 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     8761 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     8821 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     8881 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     8941 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     9001 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     9061 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     9121 aaattttcgg gtttattaca gggacagcag agatccagtt tatcgatgag gccctttcgt
     9181 cttcactcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
     9241 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
     9301 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
     9361 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
     9421 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gcgcctataa aagcagagct
     9481 cgtttagtga accgtcagat cgcctggagc aattccacaa cacttttgtc ttatacttac
     9541 gcgtgtcgac catatgacta gtatgggtaa ttataacccg ggccctatat attaggctaa
     9601 ctagctaact aggaattcga tatcaagctt tagctagcta actagctaac taggctcttc
     9661 cggccggggc cggggccggg gccggggccg gggccggggc cggggccggg gcc
//
