LOCUS       sequence-19028-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM
  TITLE     Activation of endogenous Cdc42 visualized in living cells.
  JOURNAL   Science. 2004 Sep 10. 305(5690):1615-9.
  PUBMED    15361624
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Sep 10. 305(5690):1615-9."
COMMENT     Sequence Label: pcDNA3-EGFP-Cdc42(T17N)
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..55,56..58,59..583)
                     /codon_start=1
                     /product="dominant negative mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (T17N)
                     /translation="MQTIKCVVVGDGAVGKNCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     promoter        complement(611..629)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(612..629)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(649..666)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    655..880
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggaattcatg cagacaatta agtgtgttgt tgtgggcgat ggtgctgttg gtaaaaattg
       61 tctcctgata tcctacacaa caaacaaatt tccatcggaa tatgtaccga ctgtttttga
      121 caactatgca gtcacagtta tgattggtgg agagccatat actcttggac tttttgatac
      181 tgcagggcaa gaggattatg acagattacg accgctgagt tatccacaaa cagatgtatt
      241 tctagtctgt ttttcagtgg tctctccatc ttcatttgaa aacgtgaaag aaaagtgggt
      301 gcctgagata actcaccact gtccaaagac tcctttcttg cttgttggga ctcaaattga
      361 tctcagagat gacccctcta ctattgagaa acttgccaag aacaaacaga agcctatcac
      421 tccagagact gctgaaaagc tggcccgtga cctgaaggct gtcaagtatg tggagtgttc
      481 tgcacttaca cagaaaggcc taaagaatgt atttgacgaa gcaatattgg ctgccctgga
      541 gcctccagaa ccgaagaaga gccgcaggtg tgtgctgcta tgactcgagc atgcatctag
      601 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
      661 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      721 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
      781 ggtgtcattc tattctgggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
      841 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
      901 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
      961 gtggtggtta cgcgcagcgt gacgt
//
