LOCUS       sequence-190282-        1295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in 
            combination with ATP1A1 sgSTOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R,
            Ciccia A
  TITLE     CRISPR-Mediated Base Editing Enables Efficient Disruption of 
            Eukaryotic Genes through Induction of STOP Codons.
  JOURNAL   Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 
            10.1016/j.molcel.2017.08.008.
  PUBMED    28890334
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 
            10.1016/j.molcel.2017.08.008."
COMMENT     Sequence Label: B270 + SPRTN sgSTOP
FEATURES             Location/Qualifiers
     source          1..1295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(97..345)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(359..434)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(455..703)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        810..913
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tngtctgact cgcgtgcact ttttcagttg ataacggact agccttattt taacttgcta
       61 tttctagctc taaaaccgac ggcctccagc tggcccggtg tttcgtcctt tccacaagat
      121 atataaagcc aagaaatcga aatactttca agttacggta agcatatgat agtccatttt
      181 aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt
      241 ttgtactaat atctttgtgt ttacagtcaa attaattcca attatctctc taacagcctt
      301 gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcaagct taaaaaaagc
      361 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
      421 tctagctcta aaaccttctg tagcagcttg gatcggtgtt tcgtcctttc cacaagatat
      481 ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa
      541 aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt
      601 gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta acagccttgt
      661 atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcttcctgc ccgaccttgg
      721 taccggatcc agtcgactga attggttcct ttaaagcctg cttttttgta cagctagcga
      781 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      841 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      901 gaaaaagaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      961 attttgcctt cctgttttgc tcacccagaa acgctggtga aagtaaagat gctgaagatc
     1021 agtgggtgcc cgagtgggtt acatcgaact ggatctcaac agcggtagat ccttgagagt
     1081 ttcgccccga gacgtttcca tgatgagcac tttaaagtct gctatgtggc gcggattatc
     1141 cgtattgacg cgggcagagc actcgtcgcc gcatacacta tctcagaatg actgtgatac
     1201 tcacagtcan aaaagcatct tacgatgcat gagtaagaga tatgcaggct gcnaacatga
     1261 agtataaanc gcgcacatna ctctgacacg attcg
//
