LOCUS       sequence-190285-        1222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  B270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in 
            combination with ATP1A1 sgSTOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R,
            Ciccia A
  TITLE     CRISPR-Mediated Base Editing Enables Efficient Disruption of 
            Eukaryotic Genes through Induction of STOP Codons.
  JOURNAL   Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 
            10.1016/j.molcel.2017.08.008.
  PUBMED    28890334
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 
            10.1016/j.molcel.2017.08.008."
COMMENT     Sequence Label: B270 + SMARCAL1 sgSTOP
FEATURES             Location/Qualifiers
     source          1..1222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(96..344)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(358..433)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(454..702)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        809..913
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatctgactc ggtgcacttt ttcagttgat aacggactag ccttatttta acttgctatt
       61 tctagctcta aaacgagcta gtcctctgat gctgcggtgt ttcgtccttt ccacaagata
      121 tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta
      181 aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt
      241 tgtactaata tctttgtgtt tacagtcaaa ttaattccaa ttatctctct aacagccttg
      301 tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcaagctt aaaaaaagca
      361 ccgactcggt gccacttttt caagttgata acggactagc cttattttaa cttgctattt
      421 ctagctctaa aaccttctgt agcagcttgg atcggtgttt cgtcctttcc acaagatata
      481 taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa
      541 acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg
      601 tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa cagccttgta
      661 tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcttcctgcc cgaccttggt
      721 accggatcca gtcgactgaa ttggttcctt taaagcctgc ttttttgtac agctagcgac
      781 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      841 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      901 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      961 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaga tgctgaagat
     1021 cagttgggtg ccacgagtgg gttacatcga actggatctc acagcggtag atccttgaga
     1081 gtttcgcccg aagacgtttt catgatgagc actttaaagt tctgcttggg gcgcggtatt
     1141 atcccgtatt gacgcgggca gagcactcgt cgccgcatac actattcctc agaatgactg
     1201 gttgatacct caccagttca gg
//
