LOCUS       sequence-190287-        1228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and 
            containing an empty sgRNA-expression cassette (use BbsI for 
            cloning).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R,
            Ciccia A
  TITLE     CRISPR-Mediated Base Editing Enables Efficient Disruption of 
            Eukaryotic Genes through Induction of STOP Codons.
  JOURNAL   Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 
            10.1016/j.molcel.2017.08.008.
  PUBMED    28890334
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 
            10.1016/j.molcel.2017.08.008."
COMMENT     Sequence Label: B270 (plasmid expressing ATP1A1 sgSTOP and
            con...
FEATURES             Location/Qualifiers
     source          1..1228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(94..342)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(356..431)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(452..700)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        807..911
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctgacta gcgtgcactt tttcagttga taacggacta gccttatttt aacttgctat
       61 ttctagctct aaaacaggtc ttctcgaaga cccggtgttt cgtcctttcc acaagatata
      121 taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa
      181 acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg
      241 tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa cagccttgta
      301 tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcaagcttaa aaaaagcacc
      361 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatttct
      421 agctctaaaa ccttctgtag cagcttggat cggtgtttcg tcctttccac aagatatata
      481 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      541 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      601 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
      661 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg accttggtac
      721 cggatccagt cgactgaatt ggttccttta aagcctgctt ttttgtacag ctagcgacgt
      781 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      901 aaaggaagag tatgagtatt caacatttcc gtgtcgcctt attccctttt tgcggcattt
      961 tgccttcctg ttttgctcac ccagaaacgc tggtgaagta aaagatgctg aagatcagtt
     1021 gggtgcacga gtggttacat cgaactgatc tcaacagcgg taagatcctt gagagtttcg
     1081 cccgagacgt ttccatgatg agcactttaa agtctgctat gtgcgcgtat tatccgtatg
     1141 acgccgggca gagcactcgg tcgccgcata cctattctca aatgactggt gagtattcac
     1201 agtcacgaaa agatcttacg gatggctg
//
