LOCUS       sequence-190293-        1253 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and 
            containing an empty sgRNA-expression cassette (use BsmBI for 
            cloning).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R,
            Ciccia A
  TITLE     CRISPR-Mediated Base Editing Enables Efficient Disruption of 
            Eukaryotic Genes through Induction of STOP Codons.
  JOURNAL   Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 
            10.1016/j.molcel.2017.08.008.
  PUBMED    28890334
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 
            10.1016/j.molcel.2017.08.008."
COMMENT     Sequence Label: B52 + TIMELESS sgSTOP
FEATURES             Location/Qualifiers
     source          1..1253
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(97..345)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        complement(359..434)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(454..702)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        808..912
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 antctgactc gcgtgcactt tttcagttga taacggacta gccttatttt aacttgctat
       61 ttctagctct aaaaccgctg gagggctcga gtcttcggtg tttcgtcctt tccacaagat
      121 atataaagcc aagaaatcga aatactttca agttacggta agcatatgat agtccatttt
      181 aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt
      241 ttgtactaat atctttgtgt ttacagtcaa attaattcca attatctctc taacagcctt
      301 gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcaagct taaaaaaagc
      361 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
      421 tctagctcta aaacgagacg cattaatcgt ctcggtgttt cgtcctttcc acaagatata
      481 taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa
      541 acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg
      601 tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa cagccttgta
      661 tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcttcctgcc cgaccttggt
      721 accggatcca gtcgactgaa ttggttcctt taaagcctgc ttttttgtac agctagcgac
      781 gtcaggtggc acttttcggg gaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      961 ttttgccttc ctgttttgct cacccagaaa cgctggtgaa agtaaagatg ctgaagatca
     1021 gttggtgcac gagtgggtta catcgaactg gatctcacag cggtagatcc ttgagagttt
     1081 cgcccgagac gtttccatga tgagcacttt aaagtctgct atgtggcgcg gatatccgta
     1141 tgacgcgggc agagcactcg tcgcgcatac ctatctcaga atgactgtga gtactcccag
     1201 tccagaaaag catcttacga tggcatgacg gtaagagaan tatgcagggn tgc
//
