LOCUS       sequence-190294-       11560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast SEIPIN gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC84-ScSEIPIN-Ndelta
FEATURES             Location/Qualifiers
     source          1..11560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        443..787
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1698..1726)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2321..2341
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2382..2399
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2419..2671
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2688..2704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2688..2704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2701..2723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2712..2728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2736..2742,2743..2760,2761..2766))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2781..2802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2993..3669
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3736..4761
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4802..4976
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5054..5078
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6384..6972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6873..6892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7126..7143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7158..7298
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7224..7243)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7384..7406
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7642..7836
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7902..8975)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9404..10033)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11334..11358
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(11546..11560,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(11560,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt caacatggtg gagcacgaca
       61 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      121 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      181 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      241 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
      301 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      361 gatgtgataa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
      421 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
      481 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
      541 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
      601 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
      661 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      721 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      781 agaggacctc gactctagaa ctagttaatt aaatgagttc atatattgtt gttgcatttc
      841 tgatacaatt gctaatcatt cttcctttat cgatcttaat atatcacgat ttttacctaa
      901 gactattacc tgccgattcc tctaacgtag tcccccttaa tacgttcaac attttaaatg
      961 gcgtacaatt tggtacaaaa ttcttccaat ctattaaaag cattccggta ggtacagatc
     1021 tgccgcaaac aatagacaat ggcttatcac agttaatccc catgcgtgac aacatggaat
     1081 acaagctcga tctaaaccta cagctttatt gccagagcaa aactgaccat ttaaatttag
     1141 acaatttgtt aattgatgtt tacagaggtc caggcccgct attgggtgct cctggaggaa
     1201 gtaacagcaa agatgaaaaa atctttcaca cttctagacc tattgtctgc ctcgcactga
     1261 cggattccat gtcgcctcag gagatcgaac aactaggccc atcacgtcta gacgtttacg
     1321 atgaagaatg gctaaataca ataagaatag aggacaaaat atcgttagag tcttcatatg
     1381 aaacaatctc ggtgttcttg aaaacggaga ttgcccaaag aaatctaata atacatccag
     1441 aaagtgggat taagtttagg atgaattttg agcagggatt aagaaacttg atgcttcgaa
     1501 aaagattttt atcttatatt attggcattt caattttcca ttgcataata tgtgtacttt
     1561 tttttatcac aggttgcact gcattcattt ttgttagaaa gggtcaggaa aaatccaaga
     1621 aacatagctg gcgcgcctcg agggggggcc cggtaccggt agaaaaaatg agtaaaggag
     1681 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     1741 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     1801 aatttatttg cactactgga aaactacctg ttccatggcc aaccctggtc accaccctga
     1861 cctacggcgt gcagtgcttc tcccgttacc ctgatcatat gaagcggcac gacttcttca
     1921 agagcgccat gcctgaggga tacgtgcagg agaggaccat cttcttcaag gacgacggga
     1981 actacaagac acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc
     2041 ttaagggaat cgatttcaag gaggacggaa acatcctcgg ccacaagttg gaatacaact
     2101 acaactccca caacgtatac atcatggccg acaagcaaaa gaacggcatc aaagccaact
     2161 tcaagacccg ccacaacatc gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa
     2221 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     2281 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     2341 cagctgctgg gattacacat ggcatggtag aactatacaa acaccaccac caccaccact
     2401 aagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     2461 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     2521 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     2581 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     2641 tcgcgcgcgg tgtcatctat gttactagat cgggaattcg taatcatgtc atagctgttt
     2701 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     2761 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     2821 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     2881 gggagaggcg gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc
     2941 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
     3001 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     3061 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
     3121 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
     3181 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     3241 gtgaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     3301 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     3361 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     3421 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
     3481 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     3541 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     3601 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     3661 acacgctgaa atcaccagtc tctctctaca aatctatctc tctcgagctt tcgcagatcc
     3721 ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     3781 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     3841 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     3901 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     3961 gaagtgcttg acattgggga gtttagcgag agcctgacct attgcatctc ccgccgttca
     4021 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct acaaccggtc
     4081 gcggaggcta tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     4141 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     4201 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     4261 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     4321 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     4381 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     4441 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     4501 cttgcaggat cgccacgact ccgggcgtat atgctccgca ttggtcttga ccaactctat
     4561 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     4621 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     4681 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     4741 cgtccgaggg caaagaaata gagtagatgc cgaccgggat ctgtcgatcg acaagctcga
     4801 gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
     4861 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     4921 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc
     4981 gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
     5041 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
     5101 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
     5161 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
     5221 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
     5281 attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt
     5341 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
     5401 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
     5461 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
     5521 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     5581 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     5641 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     5701 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     5761 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     5821 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     5881 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     5941 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     6001 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     6061 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     6121 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     6181 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     6241 atcaagcctg attgggagaa aataaaatat tatattatca gggacttgtt cgcaccttcc
     6301 ctagaatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6361 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6421 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6481 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6541 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6601 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6661 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6721 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6781 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6841 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6901 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6961 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7021 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7081 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7141 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     7201 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     7261 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     7321 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7381 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     7441 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     7501 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     7561 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     7621 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     7681 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     7741 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     7801 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     7861 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     7921 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     7981 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     8041 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     8101 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     8161 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     8221 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     8281 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     8341 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     8401 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
     8461 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
     8521 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
     8581 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
     8641 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
     8701 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
     8761 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
     8821 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
     8881 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
     8941 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
     9001 cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc
     9061 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
     9121 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
     9181 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
     9241 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
     9301 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
     9361 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
     9421 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
     9481 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
     9541 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
     9601 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
     9661 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
     9721 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
     9781 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
     9841 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
     9901 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
     9961 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    10021 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    10081 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    10141 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    10201 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    10261 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    10321 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    10381 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    10441 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    10501 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    10561 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    10621 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    10681 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    10741 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    10801 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    10861 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    10921 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    10981 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    11041 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    11101 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    11161 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    11221 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
    11281 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
    11341 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
    11401 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
    11461 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    11521 ttaagttggg taacgccagg gttttcccag tcacgacgtt
//
