LOCUS       sequence-190312-        6340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FtsZ tagged with mEos3.2 in E coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Lyu Z, Coltharp C, Yang X, Xiao J
  TITLE     Influence of FtsZ GTPase activity and concentration on nanoscale 
            Z-ring structure in vivo revealed by three-dimensional 
            Superresolution imaging.
  JOURNAL   Biopolymers. 2016 Oct;105(10):725-34. doi: 10.1002/bip.22895.
  PUBMED    27310678
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bip"; 
            journalName: "Biopolymers"; date: "2016-10"; volume: "105"; issue: 
            "10"; pages: "725-34"
COMMENT     Sequence Label: pJL005
FEATURES             Location/Qualifiers
     source          1..6340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(109..123,124..129,130..146,147..153)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             196..207
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1405..2085
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     terminator      2134..2228
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2298..2320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2785..2887
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2888..3547
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2954..2973)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      4098..4686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4587..4606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4840..4857
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4930..5007
                     /gene="lacI"
                     /label=lacI promoter
     CDS             5008..6090
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5027..5046)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(6139..6144,6145..6162,6163..6169)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6177..6193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6201..6217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6201..6217
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6233..6250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6233..6249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6242..6264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccgcaccgat cgcccttccc aacagttgcc taagaaacca ttattatcat gacattaacc
       61 tataaaaata ggcgtatcac gaggcccttt cgtcttcacc tcgagaaatc ataaaaaatt
      121 tatttgcttt gtgagcggat aacaattata atagattcaa ttgtgagcgg ataacaattt
      181 cacacagaat tcattaaaga ggagaaatta actactagta tgtttgaacc aatggaactt
      241 accaatgacg cggtgattaa agtcatcggc gtcggcggcg gcggcggtaa tgctgttgaa
      301 cacatggtgc gcgagcgcat tgaaggtgtt gaattcttcg cggtaaatac cgatgcacaa
      361 gcgctgcgta aaacagcggt tggacagacg attcaaatcg gtagcggtat caccaaagga
      421 ctgggcgctg gcgctaatcc agaagttggc cgcaatgcgg ctgatgagga tcgcgatgca
      481 ttgcgtgcgg cgctggaagg tgcagacatg gtctttattg ctgcgggtat gggtggtggt
      541 accggtacag gtgcagcacc agtcgtcgct gaagtggcaa aagatttggg tatcctgacc
      601 gttgctgtcg tcactaagcc tttcaacttt gaaggcaaga agcgtatggc attcgcggag
      661 caggggatca ctgaactgtc caagcatgtg gactctctga tcactatccc gaacgacaaa
      721 ctgctgaaag ttctgggccg cggtatctcc ctgctggatg cgtttggcgc agcgaacgat
      781 gtactgaaag gcgctgtgca aggtatcgct gaactgatta ctcgtccggg tttgatgaac
      841 gtggactttg cagacgtacg caccgtaatg tctgagatgg gctacgcaat gatgggttct
      901 ggcgtggcga gcggtgaaga ccgtgcggaa gaagctgctg aaatggctat ctcttctccg
      961 ctgctggaag atatcgacct gtctggcgcg cgcggcgtgc tggttaacat cacggcgggc
     1021 ttcgatctgc gtctggatga gttcgaaacg gtaggtaaca ccatccgtgc atttgcttcc
     1081 gacaacgcga ctgtggttat cggtacttct cttgacccgg atatgaatga cgagctgcgc
     1141 gtaaccgttg ttgcgacagg tatcggcatg gacaaacgtc ctgaaatcac tctggtgacc
     1201 aataagcagg ttcagcagcc agtgatggat cgctaccagc agcatgggat ggctccgctg
     1261 acccaggagc agaagccggt tgctaaagtc gtgaatgaca atgcgccgca aactgcgaaa
     1321 gagccggatt atctggatat cccagcattc ctgcgtaagc aagctgatgg atccgctggc
     1381 tccgctgctg gttctggcga attcatgagt gcgattaagc cagacatgaa gatcaaactc
     1441 cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg tacaggcaag
     1501 ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc tctgcctttt
     1561 gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc caaatatcca
     1621 gacaacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc
     1681 ttgactttcg aagacggggg catttgcaat gccagaaacg acataacaat ggaaggggac
     1741 actttctata ataaagttcg attttatggt accaactttc ccgccaatgg tccagttatg
     1801 cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg
     1861 ctgacgggtg atattgaaat ggctttgttg cttgaaggaa atgcccatta ccgatgtgac
     1921 ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcgc acactttgtg
     1981 gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa gctgtatgag
     2041 catgctgttg ctcattctgg attgcctgac aatgccagac gataagcggc cgctaagggt
     2101 cgacctgcag ccaagcttaa ttagctgagc ttggactcct gttgatagat ccagtaatga
     2161 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     2221 gtgagaatcc aagctagggg aattcatcgt gactgactga cgatctgcct cgcgcgtttc
     2281 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     2341 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     2401 cggggcgcag ccatgacccg gtcacgtagc gatagcggag tgtataattc ttgaagacga
     2461 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2521 gatccgggat gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag
     2581 gagggacagc tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta
     2641 aatcagtaag ttggcagcat cacccgacgc actttgcgcc gaataaatac ctgtgacgga
     2701 agatcacttc gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc
     2761 caacttttgg cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa
     2821 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
     2881 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     2941 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     3001 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     3061 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     3121 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     3181 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     3241 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     3301 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     3361 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     3421 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     3481 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     3541 ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggttgct acgcctgaat
     3601 aagtgataat aagcggatga atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt
     3661 tcagggcagg gtcgttaaat agccgcttat gtctattgct ggtttaccgg tttattgact
     3721 accggaagca gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc tcaggctctc
     3781 cccgtggagg taataattga cgttatgatc atttattctg cctcccagag cctgataaaa
     3841 acggttaccg gatcgagtca ggcaactatg gatgaacgaa atagacagat cgcttagata
     3901 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3961 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4021 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4081 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4141 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4201 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4261 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4321 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4381 cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg cacacagccc
     4441 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4501 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4561 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4621 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4681 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4741 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4801 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4861 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4921 ataaattccg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
     4981 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
     5041 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
     5101 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
     5161 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
     5221 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
     5281 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
     5341 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
     5401 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
     5461 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
     5521 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
     5581 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
     5641 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
     5701 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
     5761 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
     5821 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
     5881 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     5941 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     6001 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6061 caggtttccc gactggaaag cgggcagtga gcgcaacgca actaatgtaa gttagctcac
     6121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     6181 gagcggataa caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg
     6241 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6301 cacatccccc tttcgccagc tggcgtaata gcgaagaggc
//
