LOCUS       sequence-190316-       11587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast SEIPIN gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD
  TITLE     Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation 
            and Influence Lipid Droplet Proliferation.
  JOURNAL   Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 
            2015 Sep 11.
  PUBMED    26362606
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.15.00588"; journalName: "Plant Cell"; date: "2015-09"; 
            volume: "27"; issue: "9"; pages: "2616-36"
COMMENT     Sequence Label: pMDC84-ScSEIPIN
FEATURES             Location/Qualifiers
     source          1..11587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        443..787
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1737..1765)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2360..2380
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2421..2438
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2715..2731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2715..2731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2728..2750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2739..2755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2763..2769,2770..2787,2788..2793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2808..2829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3020..3696
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3763..4788
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4829..5003
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5081..5105
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6411..6999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6900..6919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7153..7170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7185..7325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7251..7270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7411..7433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7669..7863
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7929..9002)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9431..10060)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11361..11385
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(11573..11587,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(11587,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt caacatggtg gagcacgaca
       61 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      121 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      181 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      241 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
      301 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      361 gatgtgataa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
      421 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
      481 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
      541 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
      601 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
      661 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      721 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      781 agaggacctc gactctagaa ctagttaatt aaatgaaaat caatgtatcc cgtccattac
      841 agtttttaca atggagttca tatattgttg ttgcatttct gatacaattg ctaatcattc
      901 ttcctttatc gatcttaata tatcacgatt tttacctaag actattacct gccgattcct
      961 ctaacgtagt cccccttaat acgttcaaca ttttaaatgg cgtacaattt ggtacaaaat
     1021 tcttccaatc tattaaaagc attccggtag gtacagatct gccgcaaaca atagacaatg
     1081 gcttatcaca gttaatcccc atgcgtgaca acatggaata caagctcgat ctaaacctac
     1141 agctttattg ccagagcaaa actgaccatt taaatttaga caatttgtta attgatgttt
     1201 acagaggtcc aggcccgcta ttgggtgctc ctggaggaag taacagcaaa gatgaaaaaa
     1261 tctttcacac ttctagacct attgtctgcc tcgcactgac ggattccatg tcgcctcagg
     1321 agatcgaaca actaggccca tcacgtctag acgtttacga tgaagaatgg ctaaatacaa
     1381 taagaataga ggacaaaata tcgttagagt cttcatatga aacaatctcg gtgttcttga
     1441 aaacggagat tgcccaaaga aatctaataa tacatccaga aagtgggatt aagtttagga
     1501 tgaattttga gcagggatta agaaacttga tgcttcgaaa aagattttta tcttatatta
     1561 ttggcatttc aattttccat tgcataatat gtgtactttt ttttatcaca ggttgcactg
     1621 cattcatttt tgttagaaag ggtcaggaaa aatccaagaa acatagctgg cgcgcctcga
     1681 gggggggccc ggtaccggta gaaaaaatga gtaaaggaga agaacttttc actggagttg
     1741 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     1801 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     1861 aactacctgt tccatggcca accctggtca ccaccctgac ctacggcgtg cagtgcttct
     1921 cccgttaccc tgatcatatg aagcggcacg acttcttcaa gagcgccatg cctgagggat
     1981 acgtgcagga gaggaccatc ttcttcaagg acgacgggaa ctacaagaca cgtgctgaag
     2041 tcaagtttga gggagacacc ctcgtcaaca ggatcgagct taagggaatc gatttcaagg
     2101 aggacggaaa catcctcggc cacaagttgg aatacaacta caactcccac aacgtataca
     2161 tcatggccga caagcaaaag aacggcatca aagccaactt caagacccgc cacaacatcg
     2221 aagacggcgg cgtgcaactc gctgatcatt atcaacaaaa tactccaatt ggcgatggcc
     2281 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     2341 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     2401 gcatggtaga actatacaaa caccaccacc accaccacta agagctcgaa tttccccgat
     2461 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     2521 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     2581 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     2641 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     2701 ttactcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2761 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2821 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2881 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc
     2941 ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3001 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3061 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     3121 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     3181 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     3241 ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac
     3301 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     3361 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     3421 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     3481 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     3541 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     3601 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     3661 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
     3721 ctatctctct cgagctttcg cagatccggg gggcaatgag atatgaaaaa gcctgaactc
     3781 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     3841 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     3901 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     3961 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt tagcgagagc
     4021 ctgacctatt gcatctcccg ccgttcacag ggtgtcacgt tgcaagacct gcctgaaacc
     4081 gaactgcccg ctgttctaca accggtcgcg gaggctatgg atgcgatcgc tgcggccgat
     4141 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     4201 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     4261 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     4321 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     4381 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     4441 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     4501 cgctacttcg agcggaggca tccggagctt gcaggatcgc cacgactccg ggcgtatatg
     4561 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     4621 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     4681 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     4741 gatagtggaa accgacgccc cagcactcgt ccgagggcaa agaaatagag tagatgccga
     4801 ccgggatctg tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     4861 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     4921 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     4981 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
     5041 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     5101 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     5161 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     5221 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     5281 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     5341 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     5401 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     5461 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     5521 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     5581 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     5641 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     5701 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     5761 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     5821 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     5881 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     5941 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     6001 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     6061 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     6121 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     6181 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     6241 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     6301 attataatgg acgagttgtt accttcccta gaatgcatga ccaaaatccc ttaacgtgag
     6361 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6421 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6481 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6541 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     6601 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6661 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6721 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6781 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6841 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6901 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6961 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7021 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7081 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7141 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     7201 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     7261 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     7321 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7381 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7441 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     7501 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     7561 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     7621 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     7681 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     7741 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     7801 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     7861 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     7921 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     7981 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     8041 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     8101 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     8161 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     8221 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     8281 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     8341 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     8401 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     8461 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     8521 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     8581 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     8641 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     8701 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     8761 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
     8821 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     8881 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     8941 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     9001 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc
     9061 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     9121 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     9181 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     9241 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     9301 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     9361 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     9421 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     9481 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     9541 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     9601 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     9661 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     9721 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     9781 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     9841 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     9901 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     9961 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    10021 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    10081 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    10141 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    10201 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    10261 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    10321 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    10381 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    10441 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    10501 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    10561 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    10621 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    10681 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    10741 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    10801 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    10861 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    10921 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    10981 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    11041 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    11101 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    11161 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    11221 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    11281 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
    11341 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
    11401 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
    11461 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11521 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    11581 cgacgtt
//
