LOCUS       sequence-190319-       11839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mouse FIT2 gene in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Cai Y, McClinchie E, Price A, Nguyen TN, Gidda SK, Watt SC, 
            Yurchenko O, Park S, Sturtevant D, Mullen RT, Dyer JM, Chapman KD
  TITLE     Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes 
            lipid droplet accumulation in plants.
  JOURNAL   Plant Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678.
  PUBMED    27987528
REFERENCE   2  (bases 1 to 11839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678."
COMMENT     Sequence Label: pMDC32-FIT2
FEATURES             Location/Qualifiers
     source          1..11839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        116..460
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    complement(1311..1435)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1499..1751
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1768..1784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1768..1784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1781..1803)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1792..1808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1816..1822,1823..1840,1841..1846))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1861..1882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2073..2749
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             2816..3841
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    3882..4056
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4134..4158
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4583..5377
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6663..7251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7152..7171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7405..7422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7437..7577
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7503..7522)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7663..7685
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7921..8115
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8181..9254)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9683..10312)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11613..11637
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(11825..11839,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(11839,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt caacatggtg gagcacgaca
       61 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      121 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      181 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      241 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
      301 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      361 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
      421 cttcctctat ataaggaagt tcatttcatt tggagaggac ctcgactcta gaggatcccc
      481 gggtaccggg ccccccctcg aggcgcgccc accatggatc acctggagcg ctgcgcgtgg
      541 tttctccgcg ggacgctggt gagggcgacg gtgcggcgcc acctgccctg ggcgctggtg
      601 gccgccatgc tggcgggctc ggtcgtcaag gagctgtccc cgctgcccga gagctacctc
      661 agcaacaagc gcaacgtcct caacgtgtat tttgttaaat tggcctgggc ctggacagtc
      721 tgtctcctcc tgcctttcat tgcccttacc aactaccacc tgacgggcaa gaccagcctg
      781 gtcctgcggc ggttgagcac cctcctcgtg ggcacagcca tctggtatat ctgcacagcc
      841 ctcttctcca acattgagca ctatacaggc agctgctacc agtccccggc cctggagggc
      901 gtcagacagg agcatcgcag caagcagcag tgccaccggg aagggggctt ctggcatggc
      961 tttgacatct cgggccactc cttcctcttg accttctgtg cgctcatgat tgtggaagag
     1021 atggccgtgc tgcatgaggt gaagacggac cgcggccacc acctccatgc cgccatcacc
     1081 accctggttg tcgccctggg tttcctgacc ttcatctggg tgtggatgtt tctgtgcaca
     1141 gccgtttatt tccacgactt gacccagaaa gtgttcggca ccatgtttgg tttgttgggc
     1201 tggtacggaa catatgggta ttggtatctg aagtctttct ctccaggact tcctccccag
     1261 agttgtagtt tgactttgaa gcgagatact tacaagaaat gaacgtcgac catagtgact
     1321 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     1381 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttcgat
     1441 aattcttaat taactagttc tagagcggcc gccaccgcgg tggagctcga atttccccga
     1501 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     1561 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     1621 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     1681 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     1741 gttactagat cgggaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     1801 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1861 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1921 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1981 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
     2041 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
     2101 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
     2161 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     2221 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     2281 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
     2341 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     2401 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     2461 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     2521 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     2581 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     2641 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     2701 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
     2761 tctctctaca aatctatctc tctcgagctt tcgcagatcc ggggggcaat gagatatgaa
     2821 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     2881 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     2941 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     3001 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     3061 gtttagcgag agcctgacct attgcatctc ccgccgttca cagggtgtca cgttgcaaga
     3121 cctgcctgaa accgaactgc ccgctgttct acaaccggtc gcggaggcta tggatgcgat
     3181 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     3241 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     3301 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     3361 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     3421 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     3481 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     3541 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccacgact
     3601 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     3661 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     3721 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     3781 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaagaaata
     3841 gagtagatgc cgaccgggat ctgtcgatcg acaagctcga gtttctccat aataatgtgt
     3901 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     3961 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     4021 ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt
     4081 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     4141 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     4201 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     4261 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     4321 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     4381 ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
     4441 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
     4501 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
     4561 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
     4621 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     4681 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     4741 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     4801 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     4861 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     4921 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     4981 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     5041 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
     5101 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     5161 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     5221 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     5281 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     5341 aataaaatat tatattttac tggatgaatt gttttagtac ctagggaagg tgcgaataag
     5401 cggggaaatt cttctcggct gactcagtca tttcatttct tcatgtttga gccgattttt
     5461 tctcccgtaa atgccttgaa tcagcctatt tagaccgttt cttcgccatt taaggcgtta
     5521 tccccagttt ttagtgagat ctctcccact gacgtatcat ttggtccgcc cgaaacaggt
     5581 tggccagcgt gaataacatc gccagttggt tatcgttttt cagcaacccc ttgtatctgg
     5641 ctttcacgaa gccgaactgt cgcttgatga tgcgaaatgg gtgctccacc ctggcccgga
     5701 tgctggcttt catgtattcg atgttgatgg ccgttttgtt cttgcgtgga tgctgtttca
     5761 aggttcttac cttgccgggg cgctcggcga tcagccagtc cacatccacc tcggccagct
     5821 cctcgcgctg tggcgcccct tggtagccgg catcggctga gacaaattgc tcctctccat
     5881 gcagcagatt acccagctga ttgaggtcat gctcgttggc cgcggtggtg accaggctgt
     5941 gggtcaggcc actcttggca tcgacaccaa tgtgggcctt catgccaaag tgccactgat
     6001 tgcctttctt ggtctgatgc atctccggat cgcgttgctg ctctttgttc ttggtcgagc
     6061 tgggtgcctc aatgatggtg gcatcgacca aggtgccttg agtcatcatg acgcctgctt
     6121 cggccagcca gcgattgatg gtcttgaaca attggcgggc cagttgatgc tgctccagca
     6181 ggtggcggaa attcatgatg gtggtgcggt ccggcaaggc gctatccagg gataaccggg
     6241 caaacagacg catggaggcg atttcgtaca gagcatcttc catcgcgcca tcgctcaggt
     6301 tgtaccaatg ctgcatgcag tgaatgcgta gcatggtttc cagcggataa ggtcgccggc
     6361 cattaccagc cttggggtaa aacggctcga tgacttccac catgttttgc catggcagaa
     6421 tctgctccat gcgggacaag aaaatctctt ttctggtctg acggcgctta ctgctgaatt
     6481 cactgtcggc gaaggtaagt tgatgactca tgatgaaccc tgttctatgg ctccagatga
     6541 caaacatgat ctcatatcag ggacttgttc gcaccttccc tagaatgcat gaccaaaatc
     6601 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6661 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6721 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6781 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6841 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6901 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6961 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7021 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7081 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7141 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7201 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7261 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7321 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7381 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     7441 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     7501 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     7561 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7621 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7681 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     7741 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     7801 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     7861 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     7921 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     7981 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     8041 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     8101 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     8161 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
     8221 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
     8281 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
     8341 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
     8401 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
     8461 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
     8521 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
     8581 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
     8641 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
     8701 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
     8761 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
     8821 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
     8881 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
     8941 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
     9001 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
     9061 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
     9121 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
     9181 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
     9241 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
     9301 aggcaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
     9361 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
     9421 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
     9481 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
     9541 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
     9601 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
     9661 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
     9721 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
     9781 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
     9841 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
     9901 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
     9961 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    10021 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    10081 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    10141 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    10201 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    10261 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    10321 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    10381 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    10441 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    10501 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    10561 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    10621 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    10681 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    10741 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    10801 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    10861 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    10921 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    10981 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    11041 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    11101 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    11161 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    11221 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    11281 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    11341 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    11401 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    11461 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    11521 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
    11581 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
    11641 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag
    11701 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    11761 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    11821 ttttcccagt cacgacgtt
//
