LOCUS       sequence-19032-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Meng X, Brodsky MH, Wolfe SA
  TITLE     A bacterial one-hybrid system for determining the DNA-binding 
            specificity of transcription factors.
  JOURNAL   Nat Biotechnol. 2005 Aug . 23(8):988-94.
  PUBMED    16041365
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2005 Aug . 23(8):988-94."
COMMENT     Sequence Label: pH3U3-Zif268
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(57..62,63..80,81..87)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     97..119
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     116..132
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             128..790
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 gcgtgtacac ccgggcggcc gctgcgtgcg cggcactccg gaggcgcgcc gaattcttta
       61 cactttatgc ttccggctcg tatgttgtgt cgaccgagcg gataacaatt tcacacagga
      121 aacagctatg acagagcaga aagccctagt aaagcgtatt acaaatgaaa ccaagattca
      181 gattgcgatc tctttaaagg gtggtcccct agcgatagag cactcgatct tcccagaaaa
      241 agaggcagaa gcagtagcag aacaggccac acaatcgcaa gtgattaacg tccacacagg
      301 tatagggttt ctggaccata tgatacatgc tctggccaag cattccggct ggtcgctaat
      361 cgttgagtgc attggtgact tacacataga cgaccatcac accactgaag actgcgggat
      421 tgctctcggt caagctttta aagaggccct aggggccgtg cgtggagtaa aaaggtttgg
      481 atcaggattt gcgcctttgg atgaggcact ttccagagcg gtggtagatc tttcgaacag
      541 gccgtacgca gttgtcgaac ttggtttgca aagggagaaa gtaggagatc tctcttgcga
      601 gatgatcccg cattttcttg aaagctttgc agaggctagc agaattaccc tccacgttga
      661 ttgtctgcga ggcaagaatg atcatcaccg tagtgagagt gcgttcaagg ctcttgcggt
      721 tgccataaga gaagccacct cgcccaatgg taccaacgat gttccctcca ccaaaggtgt
      781 tcttatgtag tgacaccgat tatttaaagc tgcagggatc ccacacagga aacagctatg
      841 tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta
      901 ttttatatca tg
//
