LOCUS       sequence-190328-        6340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEos3.2 tagged FtsZD212A mutant in E coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Lyu Z, Coltharp C, Yang X, Xiao J
  TITLE     Influence of FtsZ GTPase activity and concentration on nanoscale 
            Z-ring structure in vivo revealed by three-dimensional 
            Superresolution imaging.
  JOURNAL   Biopolymers. 2016 Oct;105(10):725-34. doi: 10.1002/bip.22895.
  PUBMED    27310678
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bip"; 
            journalName: "Biopolymers"; date: "2016-10"; volume: "105"; issue: 
            "10"; pages: "725-34"
COMMENT     Sequence Label: pJL006
FEATURES             Location/Qualifiers
     source          1..6340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(116..130,131..136,137..153,154..160)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    136..152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             203..214
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1412..2092
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     terminator      2141..2235
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2305..2327)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2792..2894
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2895..3554
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2961..2980)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      4105..4693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4594..4613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4847..4864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4937..5014
                     /gene="lacI"
                     /label=lacI promoter
     CDS             5015..6097
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5034..5053)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(6146..6151,6152..6169,6170..6176)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6184..6200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6208..6224
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6208..6224
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6240..6257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6240..6256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6249..6271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aagaggcccg caccgatcgc ccttcccaac agttgcctaa gaaaccatta ttatcatgac
       61 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agaaatcata
      121 aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg tgagcggata
      181 acaatttcac acagaattca ttaaagagga gaaattaact actagtatgt ttgaaccaat
      241 ggaacttacc aatgacgcgg tgattaaagt catcggcgtc ggcggcggcg gcggtaatgc
      301 tgttgaacac atggtgcgcg agcgcattga aggtgttgaa ttcttcgcgg taaataccga
      361 tgcacaagcg ctgcgtaaaa cagcggttgg acagacgatt caaatcggta gcggtatcac
      421 caaaggactg ggcgctggcg ctaatccaga agttggccgc aatgcggctg atgaggatcg
      481 cgatgcattg cgtgcggcgc tggaaggtgc agacatggtc tttattgctg cgggtatggg
      541 tggtggtacc ggtacaggtg cagcaccagt cgtcgctgaa gtggcaaaag atttgggtat
      601 cctgaccgtt gctgtcgtca ctaagccttt caactttgaa ggcaagaagc gtatggcatt
      661 cgcggagcag gggatcactg aactgtccaa gcatgtggac tctctgatca ctatcccgaa
      721 cgacaaactg ctgaaagttc tgggccgcgg tatctccctg ctggatgcgt ttggcgcagc
      781 gaacgatgta ctgaaaggcg ctgtgcaagg tatcgctgaa ctgattactc gtccgggttt
      841 gatgaacgtg gactttgcag ccgtacgcac cgtaatgtct gagatgggct acgcaatgat
      901 gggttctggc gtggcgagcg gtgaagaccg tgcggaagaa gctgctgaaa tggctatctc
      961 ttctccgctg ctggaagata tcgacctgtc tggcgcgcgc ggcgtgctgg ttaacatcac
     1021 ggcgggcttc gatctgcgtc tggatgagtt cgaaacggta ggtaacacca tccgtgcatt
     1081 tgcttccgac aacgcgactg tggttatcgg tacttctctt gacccggata tgaatgacga
     1141 gctgcgcgta accgttgttg cgacaggtat cggcatggac aaacgtcctg aaatcactct
     1201 ggtgaccaat aagcaggttc agcagccagt gatggatcgc taccagcagc atgggatggc
     1261 tccgctgacc caggagcaga agccggttgc taaagtcgtg aatgacaatg cgccgcaaac
     1321 tgcgaaagag ccggattatc tggatatccc agcattcctg cgtaagcaag ctgatggatc
     1381 cgctggctcc gctgctggtt ctggcgaatt catgagtgcg attaagccag acatgaagat
     1441 caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac
     1501 aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct
     1561 gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg tattcgccaa
     1621 atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga
     1681 acgaagcttg actttcgaag acgggggcat ttgcaatgcc agaaacgaca taacaatgga
     1741 aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg ccaatggtcc
     1801 agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga
     1861 tggagtgctg acgggtgata ttgaaatggc tttgttgctt gaaggaaatg cccattaccg
     1921 atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac caggcgcaca
     1981 ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct
     2041 gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat aagcggccgc
     2101 taagggtcga cctgcagcca agcttaatta gctgagcttg gactcctgtt gatagatcca
     2161 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
     2221 tttattggtg agaatccaag ctaggggaat tcatcgtgac tgactgacga tctgcctcgc
     2281 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2341 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2401 cgggtgtcgg ggcgcagcca tgacccggtc acgtagcgat agcggagtgt ataattcttg
     2461 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2521 ttcttaggat ccgggatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc
     2581 tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca
     2641 tacactaaat cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
     2701 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
     2761 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
     2821 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     2881 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     2941 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     3001 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     3061 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
     3121 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     3181 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     3241 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     3301 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     3361 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     3421 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     3481 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     3541 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg
     3601 cctgaataag tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt
     3661 cgtcggttca gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt
     3721 attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca
     3781 ggctctcccc gtggaggtaa taattgacgt tatgatcatt tattctgcct cccagagcct
     3841 gataaaaacg gttaccggat cgagtcaggc aactatggat gaacgaaata gacagatcgc
     3901 ttagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4381 ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcac
     4441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4861 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4921 acaccgcata aattccgaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
     4981 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
     5041 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
     5101 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
     5161 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
     5221 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
     5281 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
     5341 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
     5401 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
     5461 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
     5521 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
     5581 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
     5641 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
     5701 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
     5761 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
     5821 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
     5881 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
     5941 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
     6001 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6061 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaact aatgtaagtt
     6121 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6181 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacggattca
     6241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg
//
