LOCUS       sequence-19033-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Meng X, Brodsky MH, Wolfe SA
  TITLE     A bacterial one-hybrid system for determining the DNA-binding 
            specificity of transcription factors.
  JOURNAL   Nat Biotechnol. 2005 Aug . 23(8):988-94.
  PUBMED    16041365
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2005 Aug . 23(8):988-94."
COMMENT     Sequence Label: pB1H1
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggactacaag gatgacgacg acaagttccg gaccggttcc aagacacccc cccatggtac
       61 caagccctac gtaaagatca ccgaacaacc ggcaggaaag gcgctaaggt ttcgctacga
      121 gtgcgaggga cgctcggcgg gatccattcc gggcgtgaac tctacgccgg agaacaagac
      181 ctatccgaca atcgaaattg tgggctacaa gggacgcgcc gtagttgttg tctcctgcgt
      241 cacaaaggat acgccatatc gtcctcatcc ccacaattta gttggcaagg agggctgcaa
      301 gaagggcgtc tgtacactgg agatcaacag tgagacaatg cgagcggtgt tcagtaactt
      361 gggtatccag tgtgtcaaaa agaaggacat tgaggcggcg ctcaaggcgc gcgaggagat
      421 ccgtgtggat ccgtttaaga ctggcttttc gcatcgtttc cagccctcga gcatagatct
      481 gaattcggtg cgattgtgct ttcaagtatt catggagagc gagcagaagg gtcgattcac
      541 ctcgccactg ccgccggtag tttcggagcc catcttcgat aagaaggcca tgtccgacct
      601 ggtcatctgc cggctgtgta gctgctcggc caccgttttc ggcaacaccc agatcatcct
      661 gctctgcgag aaggtggcca aggaggacat ctccgtgcga ttctttgagg agaag
//
