LOCUS       sequence-190348-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ACO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: ACO2_pLX307
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3664..3688)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3696..3737
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3848..4436
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3901..3921)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4319..4330)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4508..4741
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4819..4940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4856..4875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4910..4929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4980..5115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5135..5154)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5136..5154)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5164..5181)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5164..5180)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5173..5195)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5322..5777
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5409..5428)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5619..5640
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5803..5907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5908..5976,5977..6768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6126..6145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6939..7527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7428..7447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7681..7698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7815..7836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7851..7856,7857..7874,7875..7881)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7889..7905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7894..7916
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7913..7929
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7913..7929
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7948..7968
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7950..7968
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7996..8222
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8223..8403
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8450..8575
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9068..9301
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9486..9530
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9761..9971
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9783..9979
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9830..9965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10011..10613
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10011..10030)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10507..10527
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10656..10773
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 ccatggcgcc ctacagccta ctggtgactc ggctgcagaa agctctgggt gtgcggcagt
     1381 accatgtggc ctcagtcctg tgccaacggg ccaaggtggc gatgagccac tttgagccca
     1441 acgagtacat ccattatgac ctgctagaga agaacattaa cattgttcgc aaacgactga
     1501 accggccgct gaccctctcg gagaagattg tgtatggaca cctggatgac cccgccagcc
     1561 aggaaattga gcgaggcaag tcgtacctgc ggctgcggcc ggaccgtgtg gccatgcagg
     1621 atgcgacggc ccagatggcc atgctccagt tcatcagcag cgggctgtcc aaggtggctg
     1681 tgccatccac catccactgt gaccatctga ttgaagccca ggttgggggc gagaaagacc
     1741 tgcgccgggc caaggacatc aaccaggaag tttataattt cctggcaact gcaggtgcca
     1801 aatatggcgt gggcttctgg aagcctggat ctggaatcat tcaccagatt attctggaaa
     1861 actatgcgta ccctggtgtt cttctgattg gcactgactc ccacaccccc aatggtggcg
     1921 gccttggggg catctgcatt ggagttgggg gtgccgatgc tgtggatgtc atggctggga
     1981 tcccctggga gctgaagtgc cccaaggtga ttggcgtgaa gctgacgggc tctctctccg
     2041 gttggtcctc acccaaagat gtgatcctga aggtggcagg catcctcacg gtgaaaggtg
     2101 gcacaggtgc aatcgtggaa taccacgggc ctggtgtaga ctccatctcc tgcactggca
     2161 tggcgacaat ctgcaacatg ggtgcagaaa ttggggccac cacttccgtg ttcccttaca
     2221 accacaggat gaagaagtac ctgagcaaga ccggccggga agacattgcc aatctagctg
     2281 atgaattcaa ggatcacttg gtgcctgacc ctggctgcca ttatgaccaa ctaattgaaa
     2341 ttaacctcag tgagctgaag ccacacatca atgggccctt cacccctgac ctggctcacc
     2401 ctgtggcaga agtgggcaag gtggcagaga aggaaggatg gcctctggac atccgagtgg
     2461 gtctaattgg tagctgcacc aattcaagct atgaagatat ggggcgctca gcagctgtgg
     2521 ccaagcaggc actggcccat ggcctcaagt gcaagtccca gttcaccatc actccaggtt
     2581 ccgagcagat ccgcgccacc attgagcggg acggctatgc acagatcttg agggatctgg
     2641 gtggcattgt cctggccaat gcttgtggcc cctgcattgg ccagtgggac aggaaggaca
     2701 tcaagaaggg ggagaagaac acaatcgtca cctcctacaa caggaacttc acgggccgca
     2761 acgacgcaaa ccccgagacc catgcctttg tcacgtcccc agagattgtc acagccctgg
     2821 ccattgcggg aaccctcaag ttcaacccag agaccgacta cctgacgggc acggatggca
     2881 agaagttcag gctggaggct ccggatgcag atgagcttcc caaaggggag tttgacccag
     2941 ggcaggacac ctaccagcac ccacccaagg acagcagcgg gcagcatgtg gacgtgagcc
     3001 ccaccagcca gcgcctgcag ctcctggagc cttttgacaa gtgggatggc aaggacctgg
     3061 aggacctgca gatcctcatc aaggtcaaag ggaagtgtac cactgaccac atctcagctg
     3121 ctggcccctg gctcaagttc cgtgggcact tggataacat ctccaacaac ctgctcattg
     3181 gtgccatcaa cattgaaaac ggcaaggcca actccgtgcg caatgccgtc actcaggagt
     3241 ttggccccgt ccctgacact gcccgctact acaagaaaca tggcatcagg tgggtggtga
     3301 tcggagacga gaactacggc gagggctcga gccgggagca tgcagctctg gagcctcgcc
     3361 accttggggg ccgggccatc atcaccaaga gctttgccag gatccacgag accaacctga
     3421 agaaacaggg cctgctgcct ctgaccttcg ctgacccggc tgactacaac aagattcacc
     3481 ctgtggacaa gctgaccatt cagggcctga aggacttcac ccctggcaag cccctgaagt
     3541 gcatcatcaa gcaccccaac gggacccagg agaccatcct cctgaaccac accttcaacg
     3601 agacgcagat tgagtggttc cgcgctggca gtgccctcaa cagaatgaag gaactgcaac
     3661 agttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc
     3721 tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt
     3781 ggctttatat atcttgtgga aaggacgata ctaaaaccct tcctcggaac cgacgcgtta
     3841 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3901 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3961 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4021 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4081 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4141 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4201 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     4261 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4321 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4381 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     4441 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4501 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4561 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4621 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4681 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4741 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     4801 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4921 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     4981 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5041 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5101 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5161 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5221 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5281 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5341 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5401 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5461 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5521 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5581 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5641 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5701 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5761 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5881 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6001 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6061 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6121 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6181 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6301 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6421 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6481 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6781 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6841 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6901 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6961 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7021 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7081 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7141 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7201 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7261 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7321 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7381 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7441 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7501 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7561 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7621 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7681 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7741 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7801 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7861 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7921 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     7981 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     8041 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     8101 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     8161 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     8221 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8401 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8461 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8521 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     8581 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     8641 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     8701 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     8761 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     8821 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     8881 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     8941 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9001 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9061 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9121 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9181 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     9241 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9301 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9361 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9421 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9481 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9541 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     9601 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     9661 caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa
     9721 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9781 catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9841 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9901 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9961 aggcctaggc ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt
    10021 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
    10081 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
    10141 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
    10201 tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag
    10261 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
    10321 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
    10381 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
    10441 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
    10501 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
    10561 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc
    10621 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
    10681 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    10741 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    10801 ccactttggc gcc
//
