LOCUS       sequence-190349-        9985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of API5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: API5_pLX307
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2836..2860)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2868..2909
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3020..3608
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3073..3093)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3491..3502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3680..3913
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3991..4112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4028..4047)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4082..4101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4152..4287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4307..4326)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4308..4326)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4336..4353)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4336..4352)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4345..4367)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4494..4949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4581..4600)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4791..4812
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4975..5079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5080..5148,5149..5940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5298..5317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6111..6699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6600..6619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6853..6870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6987..7008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7023..7028,7029..7046,7047..7053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7061..7077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7066..7088
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7085..7101
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7085..7101
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7120..7140
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7122..7140
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7168..7394
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7395..7575
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7622..7747
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8240..8473
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8658..8702
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8933..9143
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8955..9151
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9002..9137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9183..9785
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9183..9202)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9679..9699
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9828..9945
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 ccatgccgac agtagaggag ctttaccgca attatggcat cctggccgat gccacggagc
     1381 aagtgggcca gcataaagat gcctatcaag tgatattgga tggtgtgaaa ggtggtacta
     1441 aggaaaagcg attagcagct caatttattc cgaaattctt taagcatttt ccagaattgg
     1501 ctgattctgc tatcaatgca cagttagacc tctgtgagga tgaagatgta tctattcgac
     1561 gtcaagcaat taaagaactg cctcaatttg ccactggaga aaatcttcct cgagtggcag
     1621 atatactaac gcaacttttg cagacagatg actctgcaga atttaaccta gtgaacaatg
     1681 ccctattaag tatatttaaa atggatgcaa aagggacttt aggtgggttg ttcagccaaa
     1741 tacttcaagg agaggacatt gttagagaac gagcaattaa attcctttct acaaaactta
     1801 agactttacc agatgaagtc ttaacaaagg aagtggaaga gcttatacta actgaatcca
     1861 aaaaggtcct agaagatgtg actggtgaag aatttgttct atttatgaag atactgtctg
     1921 ggttaaaaag cttacagaca gtgagtggaa gacagcaact tgtagagttg gtggctgaac
     1981 aggccgacct agaacagacc ttcaatccct cggatcctga ctgtgtggac aggctcttac
     2041 agtgcactcg gcaggcagta cccctcttct ctaaaaatgt ccattccaca aggtttgtga
     2101 catatttctg tgagcaggtt ctccctaacc tcggtacctt gactacccca gtggaaggtc
     2161 ttgatataca gttggaggta ttgaaattgt tggcggagat gagttcattt tgtggtgaca
     2221 tggaaaaact agaaacaaat ttaaggaaac tatttgataa gttattggaa tacatgcccc
     2281 tccctccaga agaggcagaa aatggagaga atgctggtaa tgaagaaccc aagctacagt
     2341 tcagttatgt ggaatgtttg ttgtacagtt ttcaccagtt gggccgaaaa cttccagatt
     2401 tcttaacagc caaactgaat gcagaaaagc tcaaagattt caaaatcagg ctgcagtact
     2461 ttgcacgggg cctgcaagtt tatatcagac aacttcgctt agctctccag ggtaaaacgg
     2521 gtgaggcctt aaaaacagaa gagaacaaga ttaaagtcgt tgcattgaaa ataacaaaca
     2581 atatcaatgt tttaatcaag gatctcttcc acattcctcc ttcttataag agcacagtaa
     2641 cactatcctg gaaacctgta caaaaggttg agattgggca aaagagagcc agtgaagata
     2701 caacttcagg ttcaccaccc aagaaatctt cagcaggacc aaaaagagat gccaggcaga
     2761 tttataaccc tcccagtggg aaatatagca gcaatttggg caactttaat tatgagagga
     2821 gccttcaggg gaagttgcca actttcttgt acaaagtggt tgatatcggt aagcctatcc
     2881 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa cttgaaagta
     2941 tttcgatttc ttggctttat atatcttgtg gaaaggacga tactaaaacc cttcctcgga
     3001 accgacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3061 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3121 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3181 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3241 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3301 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3361 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3421 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3481 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3541 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3601 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3661 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3721 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3781 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3841 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3901 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3961 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4021 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4081 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4141 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4201 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4261 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4321 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4381 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4441 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4501 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4561 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4621 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4681 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4741 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4801 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4861 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4921 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4981 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5041 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5101 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5161 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5221 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5281 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5341 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5401 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5461 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5521 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5581 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5641 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5701 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5761 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5821 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5881 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5941 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6781 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6901 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6961 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7021 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7081 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7141 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7201 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7261 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7321 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7381 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7441 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7501 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7561 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7621 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7681 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7741 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7801 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7861 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7921 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7981 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8041 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8101 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8161 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8221 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8281 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8341 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8401 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8461 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8521 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8581 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8641 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8701 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8761 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8821 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc
     8881 attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8941 aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9001 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9061 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9121 aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta
     9181 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     9241 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     9301 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     9361 acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg
     9421 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     9481 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     9541 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     9601 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     9661 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     9721 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca
     9781 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     9841 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     9901 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     9961 gacagcagag atccactttg gcgcc
//
