LOCUS       sequence-19035-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Meng X, Brodsky MH, Wolfe SA
  TITLE     A bacterial one-hybrid system for determining the DNA-binding 
            specificity of transcription factors.
  JOURNAL   Nat Biotechnol. 2005 Aug . 23(8):988-94.
  PUBMED    16041365
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2005 Aug . 23(8):988-94."
COMMENT     Sequence Label: pB1H2
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..87
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttcgttgact tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agaggcggcc
       61 gcggactaca aggatgacga cgacaagttc cggaccggtt ccaagacacc cccccatggt
      121 accaagccct acgtaaagat caccgaacaa ccggcaggaa aggcgctaag gtttcgctac
      181 gagtgcgagg gacgctcggc gggatccatt ccgggcgtga actctacgcc ggagaacaag
      241 acctatccga caatcgaaat tgtgggctac aagggacgcg ccgtagttgt tgtctcctgc
      301 gtcacaaagg atacgccata tcgtcctcat ccccacaatt tagttggcaa ggagggctgc
      361 aagaagggcg tctgtacact ggagatcaac agtgagacaa tgcgagcggt gttcagtaac
      421 ttgggtatcc agtgtgtcaa aaagaaggac attgaggcgg cgctcaaggc gcgcgaggag
      481 atccgtgtgg atccgtttaa gactggcttt tcgcatcgtt tccagccctc gagcatagat
      541 ctgaattcgg tgcgattgtg ctttcaagta ttcatggaga gcgagcagaa gggtcgattc
      601 acctcgccac tgccgccggt agtttcggag cccatcttcg ataagaaggc catgtccgac
      661 ctggtcatct gccggctgtg tagctgctcg gccaccgttt tctgcaacac ccagatcatc
      721 ctgctct
//
