LOCUS       sequence-190350-        9838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ADSS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: ADSS_pLX307
FEATURES             Location/Qualifiers
     source          1..9838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2721..2762
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2873..3461
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2926..2946)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3344..3355)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3533..3766
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3844..3965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3881..3900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3935..3954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4005..4140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4160..4179)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4161..4179)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4189..4206)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4189..4205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4198..4220)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4347..4802
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4434..4453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4644..4665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4828..4932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4933..5001,5002..5793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5151..5170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5964..6552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6453..6472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6706..6723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6840..6861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6876..6881,6882..6899,6900..6906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6914..6930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6919..6941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6938..6954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6938..6954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6973..6993
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6975..6993
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7021..7247
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7248..7428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7475..7600
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8093..8326
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8511..8555
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8786..8996
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8808..9004
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8855..8990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9036..9638
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9036..9055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9532..9552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9681..9798
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggcgttcgc cgagacctac ccggcggcat cctccctgcc caacggcgat tgcggccgcc
     1381 ccagggcgcg gcccggagga aaccgggtga cggtggtgct cggtgcgcag tggggcgacg
     1441 aaggcaaagg gaaggtggtg gacctgctgg cgcaggacgc cgacatcgtg tgccgctgcc
     1501 agggaggaaa taatgctggc catacagttg ttgtggattc tgtggaatat gattttcatc
     1561 tcttacccag tggaataatt aatccaaatg tcactgcatt cattggaaat ggtgtggtaa
     1621 ttcatctacc tggattgttt gaagaagcag agaaaaatgt tcaaaaagga aaaggactag
     1681 aaggctggga aaaaaggctt attatatctg acagagctca tattgtattt gattttcatc
     1741 aagcagctga tggtatccag gaacaacaga gacaagaaca agcaggaaaa aatttgggta
     1801 caacaaaaaa gggcattggc ccagtttatt cgtccaaagc tgctcggagt ggactcagga
     1861 tgtgcgacct tgtttctgac tttgatggct tctctgagag gtttaaagtt ctagctaacc
     1921 aatacaaatc tatatacccc actttggaaa tagacattga aggtgaatta caaaaactca
     1981 agggttatat ggaaaagatt aaaccaatgg tgagagatgg agtttatttt ctatatgagg
     2041 ccctacatgg accaccaaag aaaatcttgg tagaaggtgc aaatgcagca ctattagata
     2101 ttgattttgg gacttaccct tttgtaacct cttcaaattg tactgttgga ggtgtttgta
     2161 ctggtttggg tatgccacct caaaatgttg gagaagtgta tggagttgtg aaagcttata
     2221 caactagagt tggtattggt gcctttccta cagagcaaga caatgaaatt ggagaattat
     2281 tacaaacaag gggtagagag tttggtgtaa ctactggaag gaaaagaaga tgtggctggt
     2341 tggacctcgt tttgctcaaa tatgctcata tgatcaatgg atttactgcg ttggcactta
     2401 ccaagttgga tattttggac atgtttacgg aaatcaaagt tggagttgct tacaagttag
     2461 atggtgaaat catacctcat atcccagcaa accaagaagt cttaaataaa gttgaagttc
     2521 aatataagac tctcccagga tggaacacag acatatcaaa tgcaagggcg tttaaagaac
     2581 tacctgttaa tgcacaaaac tatgttcgat ttattgaaga tgagcttcaa attccagtta
     2641 agtggattgg tgttggtaaa tccagagaat ctatgattca actcttttac ccaactctct
     2701 tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     2761 cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     2821 gtggaaagga cgagtcatct ccaccttgtg aaatgttacg cgttaagtcg acaatcaacc
     2881 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2941 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3001 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3061 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3121 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3181 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3241 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3301 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3361 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3421 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     3481 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3541 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     3601 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3661 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3721 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     3781 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     3841 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3901 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3961 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     4021 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4081 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4141 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     4201 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4261 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4321 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     4381 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4441 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4501 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4561 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4621 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4681 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4741 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4801 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4861 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4921 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4981 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5041 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5101 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5161 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5221 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5281 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5341 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5401 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5461 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5521 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5581 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5641 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5701 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5761 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5821 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6721 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6781 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6841 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6901 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6961 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     7021 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     7081 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     7141 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     7201 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     7261 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7321 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7381 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     7441 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     7501 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     7561 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     7621 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     7681 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     7741 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     7801 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     7861 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     7921 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7981 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     8041 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     8101 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     8161 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     8221 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     8281 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     8341 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     8401 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     8461 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     8521 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     8581 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     8641 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     8701 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     8761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     8821 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8881 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8941 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9001 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     9061 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     9121 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     9181 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     9241 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     9301 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     9361 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     9421 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     9481 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     9541 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     9601 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     9661 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     9721 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9781 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgcc
//
