LOCUS       sequence-190351-        9383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of APOE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: APOE_pLX307
FEATURES             Location/Qualifiers
     source          1..9383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(293..317)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             325..366
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    477..1065
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(530..550)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(948..959)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1137..1370
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1448..1569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1485..1504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1539..1558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1609..1744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1764..1783)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1765..1783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1793..1810)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1793..1809)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1802..1824)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1951..2406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2038..2057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2248..2269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2432..2536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2537..2605,2606..3397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2755..2774)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3568..4156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4057..4076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4310..4327
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4444..4465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4480..4485,4486..4503,4504..4510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4518..4534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4523..4545
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4542..4558
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4542..4558
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4577..4597
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4579..4597
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4625..4851
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4852..5032
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5079..5204
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5697..5930
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6115..6159
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6390..6600
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        6412..6608
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6459..6594
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6640..7242
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(6640..6659)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7136..7156
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7285..7402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7540..8718
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7771..8709
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8666..8686
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    8738..8762
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctacaggagc gggcccaggc ctggggcgag cggctgcgcg cgcggatgga ggagatgggc
       61 agccggaccc gcgaccgcct ggacgaggtg aaggagcagg tggcggaggt gcgcgccaag
      121 ctggaggagc aggcccagca gatacgcctg caggccgagg ccttccaggc ccgcctcaag
      181 agctggttcg agcccctggt ggaagacatg cagcgccagt gggccgggct ggtggagaag
      241 gtgcaggctg ccgtgggcac cagcgccgcc cctgtgccca gcgacaatca ctgcccaact
      301 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
      361 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
      421 tcttgtggaa aggacgaccg ctggaatagt acatgggcta gacgcgttaa gtcgacaatc
      481 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
      541 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
      601 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
      661 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
      721 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
      781 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
      841 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
      901 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
      961 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     1021 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     1081 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     1141 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     1201 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1261 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1321 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     1381 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     1441 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1561 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     1621 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     1681 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     1741 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     1801 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     1861 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     1921 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     1981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2161 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2221 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2281 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     2341 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     2401 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2461 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2521 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2581 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2641 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     2701 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2761 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2821 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2881 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2941 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3001 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3061 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3121 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3181 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3241 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3301 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3361 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3421 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3481 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3841 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3901 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3961 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4021 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4081 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4141 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4201 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4261 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4321 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     4381 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     4441 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     4501 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     4561 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     4621 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     4681 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     4741 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     4801 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     4861 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4921 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4981 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     5041 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     5101 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     5161 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     5221 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     5281 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     5341 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     5401 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     5461 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     5521 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     5581 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     5641 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     5701 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     5761 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     5821 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     5881 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     5941 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     6001 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     6061 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     6121 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     6181 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     6241 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     6301 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     6361 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     6421 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6541 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6601 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     6661 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6721 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6781 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6841 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6901 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6961 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     7021 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     7081 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7141 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     7201 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     7261 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     7321 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     7381 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     7441 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     7501 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     7561 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     7621 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     7681 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     7741 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     7801 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     7861 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     7921 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     7981 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     8041 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     8101 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     8161 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     8221 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     8281 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     8341 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     8401 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     8461 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     8521 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     8581 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     8641 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     8701 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg
     8761 catgaaggtt ctgtgggctg cgttgctggt cacattcctg gcaggatgcc aggccaaggt
     8821 ggagcaagcg gtggagacag agccggagcc cgagctgcgc cagcagaccg agtggcagag
     8881 cggccagcgc tgggaactgg cactgggtcg cttttgggat tacctgcgct gggtgcagac
     8941 actgtctgag caggtgcagg aggagctgct cagctcccag gtcacccagg aactgagggc
     9001 gctgatggac gagaccatga aggagttgaa ggcctacaaa tcggaactgg aggaacaact
     9061 gaccccggtg gcggaggaga cgcgggcacg gctgtccaag gagctgcagg cggcgcaggc
     9121 ccggctgggc gcggacatgg aggacgtgtg cggccgcctg gtgcagtacc gcggcgaggt
     9181 gcaggccatg ctcggccaga gcaccgagga gctgcgggtg cgcctcgcct cccacctgcg
     9241 caagctgcgt aagcggctcc tccgcgatgc cgatgacctg cagaagcgcc tggcagtgta
     9301 ccaggccggg gcccgcgagg gcgccgagcg cggcctcagc gccatccgcg agcgcctggg
     9361 gcccctggtg gaacagggcc gcg
//
