LOCUS       sequence-190352-       10069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BMPR1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: BMPR1A_pLX307
FEATURES             Location/Qualifiers
     source          1..10069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2920..2944)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2952..2993
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3104..3692
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3157..3177)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3575..3586)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3764..3997
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4075..4196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4112..4131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4166..4185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4236..4371
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4391..4410)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4392..4410)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4420..4437)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4420..4436)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4429..4451)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4578..5033
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4665..4684)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4875..4896
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5059..5163
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5164..5232,5233..6024)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5382..5401)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6195..6783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6684..6703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6937..6954
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7071..7092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7107..7112,7113..7130,7131..7137)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7145..7161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7150..7172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7169..7185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7169..7185
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7204..7224
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7206..7224
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7252..7478
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7479..7659
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7706..7831
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8324..8557
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8742..8786
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9017..9227
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9039..9235
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9086..9221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9267..9869
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9267..9286)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9763..9783
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9912..10029
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgcctcagct atacatttac atcagattat tgggagccta tttgttcatc atttctcgtg
     1381 ttcaaggaca gaatctggat agtatgcttc atggcactgg gatgaaatca gactccgacc
     1441 agaaaaagtc agaaaatgga gtaaccttag caccagagga taccttgcct tttttaaagt
     1501 gctattgctc agggcactgt ccagatgatg ctattaataa cacatgcata actaatggac
     1561 attgctttgc catcatagaa gaagatgacc agggagaaac cacattagct tcagggtgta
     1621 tgaaatatga aggatctgat tttcagtgca aagattctcc aaaagcccag ctacgccgga
     1681 caatagaatg ttgtcggacc aatttatgta accagtattt gcaacccaca ctgccccctg
     1741 ttgtcatagg tccgtttttt gatggcagca ttcgatggct ggttttgctc atttctatgg
     1801 ctgtctgcat aattgctatg atcatcttct ccagctgctt ttgttacaaa cattattgca
     1861 agagcatctc aagcagacgt cgttacaatc gtgatttgga acaggatgaa gcatttattc
     1921 cagttggaga atcactaaaa gaccttattg accagtcaca aagttctggt agtgggtctg
     1981 gactaccttt attggttcag cgaactattg ccaaacagat tcagatggtc cggcaagttg
     2041 gtaaaggccg atatggagaa gtatggatgg gcaaatggcg tggcgaaaaa gtggcggtga
     2101 aagtattctt taccactgaa gaagccagct ggtttcgaga aacagaaatc taccaaactg
     2161 tgctaatgcg ccatgaaaac atacttggtt tcatagcggc agacattaaa ggtacaggtt
     2221 cctggactca gctctatttg attactgatt accatgaaaa tggatctctc tatgacttcc
     2281 tgaaatgtgc tacactggac accagagccc tgcttaaatt ggcttattca gctgcctgtg
     2341 gtctgtgcca cctgcacaca gaaatttatg gcacccaagg aaagcccgca attgctcatc
     2401 gagacctaaa gagcaaaaac atcctcatca agaaaaatgg gagttgctgc attgctgacc
     2461 tgggccttgc tgttaaattc aacagtgaca caaatgaagt tgatgtgccc ttgaatacca
     2521 gggtgggcac caaacgctac atggctcccg aagtgctgga cgaaagcctg aacaaaaacc
     2581 acttccagcc ctacatcatg gctgacatct acagcttcgg cctaatcatt tgggagatgg
     2641 ctcgtcgttg tatcacagga gggatcgtgg aagaatacca attgccatat tacaacatgg
     2701 taccgagtga tccgtcatac gaagatatgc gtgaggttgt gtgtgtcaaa cgtttgcggc
     2761 caattgtgtc taatcggtgg aacagtgatg aatgtctacg agcagttttg aagctaatgt
     2821 cagaatgctg ggcccacaat ccagcctcca gactcacagc attgagaatt aagaagacgc
     2881 ttgccaagat ggttgaatcc caagatgtaa aaatcagacc cccaactttc ttgtacaaag
     2941 tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     3001 gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     3061 acgatactaa aacccttcct cggaaccgac gcgttaagtc gacaatcaac ctctggatta
     3121 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3181 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3241 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3301 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3361 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3421 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3481 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3541 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3601 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3661 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     3721 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     3781 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     3841 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3901 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3961 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     4021 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     4081 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4141 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4201 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4261 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4321 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     4381 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     4441 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4501 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4561 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4621 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4681 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4741 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4801 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4861 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4921 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4981 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     5041 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5101 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5161 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5221 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5281 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5341 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5401 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5461 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5521 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5581 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5641 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5701 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5761 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5821 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5881 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5941 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6001 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6061 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6121 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6301 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6601 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6841 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6901 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6961 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7021 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7081 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7141 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7201 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     7261 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     7321 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     7381 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     7441 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     7501 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7561 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7621 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7681 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7741 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7801 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7861 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7921 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7981 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     8041 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     8101 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     8161 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     8221 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     8281 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     8341 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     8401 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     8461 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     8521 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     8581 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     8641 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8701 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8761 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     8821 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     8881 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     8941 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
     9001 gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac
     9061 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9121 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9181 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9241 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     9301 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     9361 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     9421 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     9481 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     9541 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     9601 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     9661 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     9721 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     9781 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     9841 gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta
     9901 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     9961 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
    10021 caaaattttc gggtttatta cagggacagc agagatccac tttggcgcc
//
