LOCUS       sequence-190354-       13444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IQGAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: IQGAP1_pLX307
FEATURES             Location/Qualifiers
     source          1..13444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6295..6319)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6327..6368
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6479..7067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6532..6552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6950..6961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7139..7372
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7450..7571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7487..7506)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7541..7560
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7611..7746
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7766..7785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7767..7785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7795..7812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7795..7811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7804..7826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7953..8408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8040..8059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8250..8271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8434..8538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8539..8607,8608..9399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8757..8776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9570..10158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10059..10078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10312..10329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10446..10467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10482..10487,10488..10505,10506..10512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10520..10536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10525..10547
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10544..10560
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10544..10560
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10579..10599
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10581..10599
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10627..10853
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10854..11034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11081..11206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11699..11932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12117..12161
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12392..12602
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12414..12610
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12461..12596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12642..13244
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12642..12661)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13138..13158
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13287..13404
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgtccgccgc agacgaggtt gacgggctgg gcgtggcccg gccgcactat ggctctgtcc
     1381 tggataatga aagacttact gcagaggaga tggatgaaag gagacgtcag aacgtggctt
     1441 atgagtacct ttgtcatttg gaagaagcga agaggtggat ggaagcatgc ctaggggaag
     1501 atctgcctcc caccacagaa ctggaggagg ggcttaggaa tggggtctac cttgccaaac
     1561 tggggaactt cttctctccc aaagtagtgt ccctgaaaaa aatctatgat cgagaacaga
     1621 ccagatacaa ggcgactggc ctccacttta gacacactga taatgtgatt cagtggttga
     1681 atgccatgga tgagattgga ttgcctaaga ttttttaccc agaaactaca gatatctatg
     1741 atcgaaagaa catgccaaga tgtatctact gtatccatgc actcagtttg tacctgttca
     1801 agctaggcct ggcccctcag attcaagacc tatatggaaa ggttgacttc acagaagaag
     1861 aaatcaacaa catgaagact gagttggaga agtatggcat ccagatgcct gcctttagca
     1921 agattggggg catcttggct aatgaactgt cagtggatga agccgcatta catgctgctg
     1981 ttattgctat taatgaagct attgaccgta gaattccagc cgacacattt gcagctttga
     2041 aaaatccgaa tgccatgctt gtaaatcttg aagagccctt ggcatccact taccaggata
     2101 tactttacca ggctaagcag gacaaaatga caaatgctaa aaacaggaca gaaaactcag
     2161 agagagaaag agatgtttat gaggagctgc tcacgcaagc tgaaattcaa ggcaatataa
     2221 acaaagtcaa tacattttct gcattagcaa atatcgacct ggctttagaa caaggagatg
     2281 cactggcctt gttcagggct ctgcagtcac cagccctggg gcttcgagga ctgcagcaac
     2341 agaatagcga ctggtacttg aagcagctcc tgagtgataa acagcagaag agacagagtg
     2401 gtcagactga ccccctgcag aaggaggagc tgcagtctgg agtggatgct gcaaacagtg
     2461 ctgcccagca atatcagaga agattggcag cagtagcact gattaatgct gcaatccaga
     2521 agggtgttgc tgagaagact gttttggaac tgatgaatcc cgaagcccag ctgccccagg
     2581 tgtatccatt tgccgccgat ctctatcaga aggagctggc taccctgcag cgacaaagtc
     2641 ctgaacataa tctcacccac ccagagctct ctgtcgcagt ggagatgttg tcatcggtgg
     2701 ccctgatcaa cagggcattg gaatcaggag atgtgaatac agtgtggaag caattgagca
     2761 gttcagttac tggtcttacc aatattgagg aagaaaactg tcagaggtat ctcgatgagt
     2821 tgatgaaact gaaggctcag gcacatgcag agaataatga attcattaca tggaatgata
     2881 tccaagcttg cgtggaccat gtgaacctgg tggtgcaaga ggaacatgag aggattttag
     2941 ccattggttt aattaatgaa gccctggatg aaggtgatgc ccaaaagact ctgcaggccc
     3001 tacagattcc tgcagctaaa cttgagggag tccttgcaga agtggcccag cattaccaag
     3061 acacgctgat tagagcgaag agagagaaag cccaggaaat ccaggatgag tcagctgtgt
     3121 tatggttgga tgaaattcaa ggtggaatct ggcagtccaa caaagacacc caagaagcac
     3181 agaagtttgc cttaggaatc tttgccatta atgaggcagt agaaagtggt gatgttggca
     3241 aaacactgag tgcccttcgc tcccctgatg ttggcttgta tggagtcatc cctgagtgtg
     3301 gtgaaactta ccacagtgat cttgctgaag ccaagaagaa aaaactggca gtaggagata
     3361 ataacagcaa gtgggtgaag cactgggtaa aaggtggata ttattattac cacaatctgg
     3421 agacccagga aggaggatgg gatgaacctc caaattttgt gcaaaattct atgcagcttt
     3481 ctcgggagga gatccagagt tctatctctg gggtgactgc cgcatataac cgagaacagc
     3541 tgtggctggc caatgaaggc ctgatcacca ggctgcaggc tcgctgccgt ggatacttag
     3601 ttcgacagga attccgatcc aggatgaatt tcctgaagaa acaaatccct gccatcacct
     3661 gcattcagtc acagtggaga ggatacaagc agaagaaggc atatcaagat cggttagctt
     3721 acctgcgctc ccacaaagat gaagttgtaa agattcagtc cctggcaagg atgcaccaag
     3781 ctcgaaagcg ctatcgagat cgcctgcagt acttccggga ccatataaat gacattatca
     3841 aaatccaggc ttttattcgg gcaaacaaag ctcgggatga ctacaagact ctcatcaatg
     3901 ctgaggatcc tcctatggtt gtggtccgaa aatttgtcca cctgctggac caaagtgacc
     3961 aggattttca ggaggagctt gaccttatga agatgcggga agaggttatc accctcattc
     4021 gttctaacca gcagctggag aatgacctca atctcatgga tatcaaaatt ggactgctag
     4081 tgaaaaataa gattacgttg caggatgtgg tttcccacag taaaaaactt accaaaaaaa
     4141 ataaggaaca gttgtctgat atgatgatga taaataaaca gaagggaggt ctcaaggctt
     4201 tgagcaagga gaagagagag aagttggaag cttaccagca cctgttttat ttattgcaaa
     4261 ccaatcccac ctatctggcc aagctcattt ttcagatgcc ccagaacaag tccaccaagt
     4321 tcatggactc tgtaatcttc acactctaca actacgcgtc caaccagcga gaggagtacc
     4381 tgctcctgcg gctctttaag acagcactcc aagaggaaat caagtcgaag gtagatcaga
     4441 ttcaagagat tgtgacagga aatcctacgg ttattaaaat ggttgtaagt ttcaaccgtg
     4501 gtgcccgtgg ccagaatgcc ctgagacaga tcttggcccc agtcgtgaag gaaattatgg
     4561 atgacaaatc tctcaacatc aaaactgacc ctgtggatat ttacaaatct tgggttaatc
     4621 agatggagtc tcagacagga gaggcaagca aactgcccta tgatgtgacc cctgagcagg
     4681 cgctagctca tgaagaagtg aagacacggc tagacagctc catcaggaac atgcgggctg
     4741 tgacagacaa gtttctctca gccattgtca gctctgtgga caaaatccct tatgggatgc
     4801 gcttcattgc caaagtgctg aaggactcgt tgcatgagaa gttccctgat gctggtgagg
     4861 atgagctgct gaagattatt ggtaacttgc tttattatcg atacatgaat ccagccattg
     4921 ttgctcctga tgcctttgac atcattgacc tgtcagcagg aggccagctt accacagacc
     4981 aacgccgaaa tctgggctcc attgcaaaaa tgcttcagca tgctgcttcc aataagatgt
     5041 ttctgggaga taatgcccac ttaagcatca ttaatgaata tctttcccag tcctaccaga
     5101 aattcagacg gtttttccaa actgcttgtg atgtcccaga gcttcaggat aaatttaatg
     5161 tggatgagta ctctgattta gtaaccctca ccaaaccagt aatctacatt tccattggtg
     5221 aaatcatcaa cacccacact ctcctgttgg atcaccagga tgccattgct ccggagcaca
     5281 atgatccaat ccacgaactg ctggacgacc tcggcgaggt gcccaccatc gagtccctga
     5341 taggggaaag ctctggcaat ttaaatgacc caaataagga ggcactggct aagacggaag
     5401 tgtctctcac cctgaccaac aagttcgacg tgcctggaga tgagaatgca gaaatggatg
     5461 ctcgaaccat cttactgaat acaaaacgtt taattgtgga tgtcatccgg ttccagccag
     5521 gagagacctt gactgaaatc ctagaaacac cagccaccag tgaacaggaa gcagaacatc
     5581 agagagccat gcagagacgt gctatccgtg atgccaaaac acctgacaag atgaaaaagt
     5641 caaaatctgt aaaggaagac agcaacctca ctcttcaaga gaagaaagag aagatccaga
     5701 caggtttaaa gaagctaaca gagcttggaa ccgtggaccc aaagaacaaa taccaggaac
     5761 tgatcaacga cattgccagg gatattcgga atcagcggag gtaccgacag aggagaaagg
     5821 ccgaactagt gaaactgcaa cagacatacg ctgctctgaa ctctaaggcc accttttatg
     5881 gggagcaggt ggattactat aaaagctata tcaaaacctg cttggataac ttagccagca
     5941 agggcaaagt ctccaaaaag cctagggaaa tgaaaggaaa gaaaagcaaa aagatttctc
     6001 tgaaatatac agcagcaaga ctacatgaaa aaggagttct tctggaaatt gaggacctgc
     6061 aagtgaatca gtttaaaaat gttatatttg aaatcagtcc aacagaagaa gttggagact
     6121 tcgaagtgaa agccaaattc atgggagttc aaatggagac ttttatgtta cattatcagg
     6181 acctgctgca gctacagtat gaaggagttg cagtcatgaa attatttgat agagctaaag
     6241 taaatgtcaa cctcctgatc ttccttctca acaaaaagtt ctacgggaag agacccccaa
     6301 ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg
     6361 attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata
     6421 tatcttgtgg aaaggacgat actaaaaccc ttcctcggaa ccgacgcgtt aagtcgacaa
     6481 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6541 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6601 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6661 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6721 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6781 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6841 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6901 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6961 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7021 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     7081 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7141 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7201 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7261 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7321 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     7381 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7441 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7501 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7561 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     7621 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7681 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7741 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     7801 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7861 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7921 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     7981 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8041 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8101 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8161 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8221 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8281 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8341 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     8401 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8461 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8521 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8581 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8641 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8701 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8761 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8821 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8881 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8941 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9001 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9061 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     9121 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9181 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9241 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9301 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9361 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9421 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9481 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9541 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9601 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9661 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9721 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9781 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9841 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9901 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9961 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10021 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10081 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10141 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10201 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10261 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10321 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10381 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10441 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10501 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    10561 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
    10621 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
    10681 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
    10741 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
    10801 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
    10861 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10921 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10981 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
    11041 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
    11101 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
    11161 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
    11221 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
    11281 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
    11341 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    11401 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    11461 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    11521 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
    11581 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    11641 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    11701 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    11761 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    11821 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    11881 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    11941 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    12001 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    12061 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    12121 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    12181 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    12241 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
    12301 cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc
    12361 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct
    12421 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    12481 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    12541 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    12601 cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca
    12661 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
    12721 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
    12781 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    12841 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    12901 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    12961 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    13021 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    13081 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    13141 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    13201 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg
    13261 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    13321 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    13381 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
    13441 cgcc
//
