LOCUS       sequence-190355-        9164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BCL2L1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: BCL2L1_pLX307
FEATURES             Location/Qualifiers
     source          1..9164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2026..2050)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2058..2099
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2199..2787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2252..2272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2670..2681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2859..3092
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3170..3291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3207..3226)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3261..3280
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3331..3466
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3486..3505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3487..3505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3515..3532)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3515..3531)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3524..3546)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3673..4128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3760..3779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3970..3991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4154..4258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4259..4327,4328..5119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4477..4496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5290..5878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5779..5798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6032..6049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6166..6187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6202..6207,6208..6225,6226..6232)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6240..6256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6245..6267
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6264..6280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6264..6280
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6299..6319
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6301..6319
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6347..6573
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6574..6754
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6801..6926
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7419..7652
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7837..7881
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8112..8322
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8134..8330
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8181..8316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8362..8964
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8362..8381)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8858..8878
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9007..9124
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagcaggca
     1321 ccatgtctca gagcaaccgg gagctggtgg ttgactttct ctcctacaag ctttcccaga
     1381 aaggatacag ctggagtcag tttagtgatg tggaagagaa caggactgag gccccagaag
     1441 ggactgaatc ggagatggag acccccagtg ccatcaatgg caacccatcc tggcacctgg
     1501 cagacagccc cgcggtgaat ggagccactg gccacagcag cagtttggat gcccgggagg
     1561 tgatccccat ggcagcagta aagcaagcgc tgagggaggc aggcgacgag tttgaactgc
     1621 ggtaccggcg ggcattcagt gacctgacat cccagctcca catcacccca gggacagcat
     1681 atcagagctt tgaacaggta gtgaatgaac tcttccggga tggggtaaac tggggtcgca
     1741 ttgtggcctt tttctccttc ggcggggcac tgtgcgtgga aagcgtagac aaggagatgc
     1801 aggtattggt gagtcggatc gcagcttgga tggccactta cctgaatgac cacctagagc
     1861 cttggatcca ggagaacggc ggctgggata cttttgtgga actctatggg aacaatgcag
     1921 cagccgagag ccgaaagggc caggaacgct tcaaccgctg gttcctgacg ggcatgactg
     1981 tggccggcgt ggttctgctg ggctcactct tcagtcggaa atagaaccca gctttcttgt
     2041 acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
     2101 agtaatgaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     2161 atgctaccac agagaaagca atcacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     2221 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2281 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2341 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2401 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2461 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     2521 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2581 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     2641 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     2701 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     2761 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     2821 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     2881 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     2941 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3001 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3061 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     3121 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3301 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3361 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3421 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     3481 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     3541 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3601 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3661 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3721 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3781 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3841 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3901 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3961 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4021 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4081 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     4141 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4201 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4261 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4321 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4381 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4441 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4501 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4561 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4621 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4681 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4741 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4801 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4861 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4921 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4981 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5041 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5101 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5161 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5341 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5401 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5461 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5521 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5641 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5821 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5881 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5941 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6001 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6061 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6121 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6181 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6241 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     6301 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     6361 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     6421 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     6481 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     6541 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     6601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6721 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     6781 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     6841 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     6901 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     6961 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7021 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     7081 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     7141 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     7201 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     7261 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     7321 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     7381 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     7441 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     7501 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7561 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     7621 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7681 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     7741 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     7801 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     7861 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     7921 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     7981 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8041 accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt ggaaagtccc
     8101 caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca gcaaccatag
     8161 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8221 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8281 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttcta
     8341 gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     8401 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     8461 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     8521 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg
     8581 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8641 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8701 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8761 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8821 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     8881 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8941 acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg cagtattcat
     9001 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     9061 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     9121 ttttcgggtt tattacaggg acagcagaga tccactttgg cgcc
//
