LOCUS       sequence-190356-        9586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CDK9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: CDK9_pLX307
FEATURES             Location/Qualifiers
     source          1..9586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2437..2461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2469..2510
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2621..3209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2674..2694)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3092..3103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3281..3514
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3592..3713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3629..3648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3683..3702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3753..3888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3908..3927)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3909..3927)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3937..3954)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3937..3953)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3946..3968)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4095..4550
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4182..4201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4392..4413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4576..4680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4681..4749,4750..5541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4899..4918)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5712..6300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6201..6220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6454..6471
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6588..6609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6624..6629,6630..6647,6648..6654)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6662..6678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6667..6689
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6686..6702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6686..6702
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6721..6741
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6723..6741
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6769..6995
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6996..7176
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7223..7348
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7841..8074
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8259..8303
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8534..8744
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8556..8752
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8603..8738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8784..9386
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8784..8803)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9280..9300
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9429..9546
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggcgaagca gtacgactcg gtggagtgcc ctttttgtga tgaagtttcc aaatacgaga
     1381 agctcgccaa gatcggccaa ggcaccttcg gggaggtgtt caaggccagg caccgcaaga
     1441 ccggccagaa ggtggctctg aagaaggtgc tgatggaaaa cgagaaggag gggttcccca
     1501 ttacagcctt gcgggagatc aagatccttc agcttctaaa acacgagaat gtggtcaact
     1561 tgattgagat ttgtcgaacc aaagcttccc cctataaccg ctgcaagggt agtatatacc
     1621 tggtgttcga cttctgcgag catgaccttg ctgggctgtt gagcaatgtt ttggtcaagt
     1681 tcacgctgtc tgagatcaag agggtgatgc agatgctgct taacggcctc tactacatcc
     1741 acagaaacaa gatcctgcat agggacatga aggctgctaa tgtgcttatc actcgtgatg
     1801 gggtcctgaa gctggcagac tttgggctgg cccgggcctt cagcctggcc aagaacagcc
     1861 agcccaaccg ctacaccaac cgtgtggtga cactctggta ccggcccccg gagctgttgc
     1921 tcggggagcg ggactacggc ccccccattg acctgtgggg tgctgggtgc atcatggcag
     1981 agatgtggac ccgcagcccc atcatgcagg gcaacacgga gcagcaccaa ctcgccctca
     2041 tcagtcagct ctgcggctcc atcacccctg aggtgtggcc aaacgtggac aactatgagc
     2101 tgtacgaaaa gctggagctg gtcaagggcc agaagcggaa ggtgaaggac aggctgaagg
     2161 cctatgtgcg tgacccatac gcactggacc tcatcgacaa gctgctggtg ctggaccctg
     2221 cccagcgcat cgacagcgat gacgccctca accacgactt cttctggtcc gaccccatgc
     2281 cctccgacct caagggcatg ctctccaccc acctgacgtc catgttcgag tacttggcac
     2341 caccgcgccg gaagggcagc cagatcaccc agcagtccac caaccagagt cgcaatcccg
     2401 ccaccaccaa ccagacggag tttgagcgcg tcttctgccc aactttcttg tacaaagtgg
     2461 ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgaa
     2521 ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     2581 atgcattgat tcacatcgac tactcacgcg ttaagtcgac aatcaacctc tggattacaa
     2641 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2701 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2761 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2821 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2881 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2941 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3001 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3061 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3121 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3181 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     3241 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     3301 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     3361 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3421 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3481 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     3541 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     3601 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3661 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3721 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3781 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3841 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     3901 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     3961 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4021 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4081 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4441 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4501 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4621 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4681 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4741 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4801 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4861 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4921 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4981 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5041 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5101 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5161 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5221 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5281 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5341 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5401 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5461 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5521 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5581 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5641 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5821 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6061 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6361 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6421 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6481 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6541 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6601 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6661 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6721 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     6781 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     6841 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     6901 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     6961 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     7021 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7081 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7141 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7201 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7261 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7321 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7381 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7441 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7501 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7561 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7621 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7681 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7741 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7801 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7861 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7921 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7981 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8041 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8101 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8161 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8221 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8281 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8341 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8401 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8461 caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc
     8521 cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat
     8581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc
     8761 tagaggtacc accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     8821 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     8881 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     8941 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg
     9001 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     9061 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     9121 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     9181 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     9241 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     9301 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     9361 tgacccgcaa gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc
     9421 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     9481 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     9541 aattttcggg tttattacag ggacagcaga gatccacttt ggcgcc
//
