LOCUS       sequence-19036-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Huth JR, Bewley CA, Jackson BM, Hinnebusch AG, Clore GM, Gronenborn 
            AM
  TITLE     Design of an expression system for detecting folded protein domains 
            and mapping macromolecular interactions by NMR.
  JOURNAL   Protein Sci. 1997 Nov . 6(11):2359-64.
  PUBMED    9385638
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 1997 Nov . 6(11):2359-64."
COMMENT     Sequence Label: GEV 1
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(455..616)
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLALNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     RBS             630..652
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    667..691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(691..710)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(692..710)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt
       61 acttttttcg catgctgggg ccatatagtt ccacaaaggc atcccagcct ccgttatcct
      121 ggatccaggt gtgcagatgc cggttcaggt actcagtcat ccacagggcg atgttgtcca
      181 ccaggggtga catctccctg ttgacgctct ccacacacat gaccccaccg aactcaaaga
      241 aggccacaat cctcccccag ttcaccccat ccctgaagag ttcctccacc accgtggcaa
      301 agcgtcccct cgcggtgaag ggcgtcaggt gcagctgact ggacatctct gcgaagtcac
      361 gacggtagcg acgagagaag tcatccccag cccggcggag ggtcagatgg accacaggtg
      421 gcacagggct gagcgcgcta gccggaccgc ccggttcggt taccgtgaag gttttggtag
      481 cgtcgtcgta ggtccattca ccgtcaacac cgttgtcgtt agcgtactgt ttgaaaactt
      541 tttccgcggt agcagcgtca acagcttcgg tggtggtttc acctttcagg gttttaccgt
      601 tcagagcaag cttgtactgc atatgtatat ctccttctta aagttaaaca aaattatttc
      661 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg
      721 atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      781 gcggttgctg g
//
