LOCUS       sequence-190369-        3240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for NKX2-5 YRD Y-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pENTR-2B-Nkx2-5YRD Y-A
FEATURES             Location/Qualifiers
     source          1..3240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1112..1211)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1323..1342)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1334..2143
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1401..1420)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2233..2821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2722..2741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2986..3072
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3164..3191
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3199..3222
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt
      121 cgactggatc cgatgttccc cagccctgcg ctcacaccca cgcctttctc agtcaaagac
      181 atcctgaacc tggagcagca gcagcgtagc ctggcgtctg gggacctgtc tgcgcgcctc
      241 gaggccaccc tggcccctgc ctcctgcatg ctggccgcct tcaagcccga ggcctactct
      301 ggccccgagg cggcagcgtc cggcctggca gagctgcgcg cggagatggg ccccgcgcct
      361 tcgcccccca agtgctctcc tgctttccca gccgccccca cattttaccc gggagcctac
      421 ggtgaccctg acccagccaa agaccctcgg gcggataaaa aagagctgtg cgcgctgcag
      481 aaggcagtgg agctggacaa agccgagacg gatggcgccg agagaccacg cgcacggcgg
      541 cgacggaagc cacgcgtgct cttctcgcag gcgcaggtct acgagctgga gcggcgcttc
      601 aagcaacagc ggtacctgtc ggcgccagag cgcgaccagc tggccagcgt gctgaagctc
      661 acgtccacgc aggtcaagat ctggttccag aaccgtcgct acaagtgcaa gcgacagcgg
      721 caggaccaga ctctggagct tctggggccg ccgccgccgc ccgcgcgcag gatcgcggtg
      781 cccgtgctgg tgcgcgacgg gaagccctgc ctgggggacc ccgcggccgc cgctcccgcc
      841 gccggcgtgg gtctcaatgc cgctggcgcc aacgccgccc ccgcccccag cgccggcggc
      901 gcggcctgca gtcccggcta cagctgcgcc gcctaccccg ctgcgccccc cgccgcgcag
      961 ccccccgccg cctccgccaa cagcaacttc gtgaactttg gcgtcgggga cttgaacacc
     1021 gtgcagagtc ccgggatgcc gcagggcaat tcgggcgtct ccacgctgca cggcatccga
     1081 gcctggtagc ggccgcactc gagatatcta gacccagctt tcttgtacaa agttggcatt
     1141 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1201 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
     1261 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1321 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     1381 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1441 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1501 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     1561 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1621 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1681 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     1741 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     1801 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     1861 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     1921 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     1981 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2041 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2101 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     2161 gttgtaacat tattcagatt gggccccgtt ccactgagcg tcagaccccg tagaaaagat
     2221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2341 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta gcatggatct
     2941 cggggacgtc taactactaa gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     3001 gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     3061 agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg
     3121 cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg
     3181 gatggccttt ttgcgtttct acaaactctt cctgttagtt agttacttaa gctcgggccc
//
