LOCUS       sequence-19037-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Huth JR, Bewley CA, Jackson BM, Hinnebusch AG, Clore GM, Gronenborn 
            AM
  TITLE     Design of an expression system for detecting folded protein domains 
            and mapping macromolecular interactions by NMR.
  JOURNAL   Protein Sci. 1997 Nov . 6(11):2359-64.
  PUBMED    9385638
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 1997 Nov . 6(11):2359-64."
COMMENT     Sequence Label: GEV 2
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(470..487)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(488..649)
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLALNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     RBS             663..685
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    700..724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(724..743)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(725..743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattct taaggattaa aatcatgctc ctttaaccca cctggcaaac
      121 tttcctcaat aacagaaaat ggatctctag tcaaggcact atacatcaaa tattccttat
      181 taaccccttt acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag
      241 cagacactct atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat
      301 gcctacttat aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt
      361 tttcctttgt ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac
      421 tcaaaaaact tagcaattct gaaggaaagt ccttggggtc ttctaccttg gatccacgcg
      481 gaaccagttc ggttaccgtg aaggttttgg tagcgtcgtc gtaggtccat tcaccgtcaa
      541 caccgttgtc gttagcgtac tgtttgaaaa ctttttccgc ggtagcagcg tcaacagctt
      601 cggtggtggt ttcacctttc agggttttac cgttcagagc aagcttgtac tgcatatgta
      661 tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa
      721 ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga
      781 cgcatcgtgg ccggcatcac cg
//
