LOCUS       sequence-190383-        6352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FtsZ tagged with GFP in E coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Buss J, Coltharp C, Shtengel G, Yang X, Hess H, Xiao J
  TITLE     A multi-layered protein network stabilizes the Escherichia coli 
            FtsZ-ring and modulates constriction dynamics.
  JOURNAL   PLoS Genet. 2015 Apr 7;11(4):e1005128. doi: 
            10.1371/journal.pgen.1005128. eCollection 2015 Apr.
  PUBMED    25848771
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-04-7"; volume: "11(4):e1005128. doi"; pages: " 
            10.1371/journal.pgen.1005128. eCollection 2015 Apr"
COMMENT     Sequence Label: pXY027
FEATURES             Location/Qualifiers
     source          1..6352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(116..130,131..136,137..153,154..160)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    136..152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             203..214
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(1418..1446)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2041..2061
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2153..2247
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2317..2339)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2804..2906
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2907..3566
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2973..2992)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      4117..4705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4606..4625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4859..4876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4949..5026
                     /gene="lacI"
                     /label=lacI promoter
     CDS             5027..6109
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5046..5065)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(6158..6163,6164..6181,6182..6188)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6196..6212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6220..6236
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6220..6236
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6252..6269)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6252..6268)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6261..6283)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aagaggcccg caccgatcgc ccttcccaac agttgcctaa gaaaccatta ttatcatgac
       61 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agaaatcata
      121 aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg tgagcggata
      181 acaatttcac acagaattca ttaaagagga gaaattaact actagtatgt ttgaaccaat
      241 ggaacttacc aatgacgcgg tgattaaagt catcggcgtc ggcggcggcg gcggtaatgc
      301 tgttgaacac atggtgcgcg agcgcattga aggtgttgaa ttcttcgcgg taaataccga
      361 tgcacaagcg ctgcgtaaaa cagcggttgg acagacgatt caaatcggta gcggtatcac
      421 caaaggactg ggcgctggcg ctaatccaga agttggccgc aatgcggctg atgaggatcg
      481 cgatgcattg cgtgcggcgc tggaaggtgc agacatggtc tttattgctg cgggtatggg
      541 tggtggtacc ggtacaggtg cagcaccagt cgtcgctgaa gtggcaaaag atttgggtat
      601 cctgaccgtt gctgtcgtca ctaagccttt caactttgaa ggcaagaagc gtatggcatt
      661 cgcggagcag gggatcactg aactgtccaa gcatgtggac tctctgatca ctatcccgaa
      721 cgacaaactg ctgaaagttc tgggccgcgg tatctccctg ctggatgcgt ttggcgcagc
      781 gaacgatgta ctgaaaggcg ctgtgcaagg tatcgctgaa ctgattactc gtccgggttt
      841 gatgaacgtg gactttgcag acgtacgcac cgtaatgtct gagatgggct acgcaatgat
      901 gggttctggc gtggcgagcg gtgaagaccg tgcggaagaa gctgctgaaa tggctatctc
      961 ttctccgctg ctggaagata tcgacctgtc tggcgcgcgc ggcgtgctgg ttaacatcac
     1021 ggcgggcttc gatctgcgtc tggatgagtt cgaaacggta ggtaacacca tccgtgcatt
     1081 tgcttccgac aacgcgactg tggttatcgg tacttctctt gacccggata tgaatgacga
     1141 gctgcgcgta accgttgttg cgacaggtat cggcatggac aaacgtcctg aaatcactct
     1201 ggtgaccaat aagcaggttc agcagccagt gatggatcgc taccagcagc atgggatggc
     1261 tccgctgacc caggagcaga agccggttgc taaagtcgtg aatgacaatg cgccgcaaac
     1321 tgcgaaagag ccggattatc tggatatccc agcattcctg cgtaagcaag ctgatggcct
     1381 atgcggccgc agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     1441 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     1501 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1561 aacacttgtc actactctga cgtatggtgt tcaatgcttt tcccgttatc cggatcatat
     1621 gaaacggtat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     1681 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     1741 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg
     1801 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa
     1861 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact
     1921 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1981 ccattacctg tcgacacaat ctgccctttt gaaagatccc aacgaaaagc gtgaccacat
     2041 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg aactatacaa
     2101 ataagcggcc gctaagggtc gacctgcagc caagcttaat tagctgagct tggactcctg
     2161 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
     2221 cgccgggcgt tttttattgg tgagaatcca agctagggga attcatcgtg actgactgac
     2281 gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     2401 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccgg tcacgtagcg atagcggagt
     2461 gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     2521 gataataatg gtttcttagg atccgggatg tccggcggtg cttttgccgt tacgcaccac
     2581 cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca
     2641 aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg
     2701 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg
     2761 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag
     2821 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     2881 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     2941 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     3001 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     3061 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     3121 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     3181 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     3241 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     3301 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     3361 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     3421 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     3481 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     3541 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc
     3601 ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgaaagcaa
     3661 attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg tctattgctg
     3721 gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa atgcctgagg
     3781 ccagtttgct caggctctcc ccgtggaggt aataattgac gttatgatca tttattctgc
     3841 ctcccagagc ctgataaaaa cggttaccgg atcgagtcag gcaactatgg atgaacgaaa
     3901 tagacagatc gcttagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4261 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
     4441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4741 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4801 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4861 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     4921 gtgcggtatt tcacaccgca taaattccga caccatcgaa tggcgcaaaa cctttcgcgg
     4981 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac
     5041 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
     5101 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
     5161 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
     5221 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
     5281 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
     5341 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
     5401 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
     5461 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
     5521 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
     5581 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
     5641 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg
     5701 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
     5761 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
     5821 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     5881 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     5941 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     6001 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6061 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6121 ctaatgtaag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6181 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6241 attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6301 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cg
//
