LOCUS       sequence-190389-       14040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14040)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 14040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pMADM-beta
FEATURES             Location/Qualifiers
     source          1..14040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        954..1257
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1259..1536
                     /label=chicken beta-actin promoter
     intron          1538..2555
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2479..2501
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2563..2582
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2679..5720
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             5745..6548
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5808..5827)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6418..6437
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          6648..6713
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    6873..7007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7110..7244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7347..7481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7514..7547)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7578..8077
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7613..7636)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8010..8029
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     5'UTR           8119..8158
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             8176..9306
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(9304..9323)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9348..9396
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    10504..10551
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(10770..11240)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWSSARLPNSPYSESYYNSLAVVLQRRD
                     WENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVT
                     RSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHRAR"
     primer_bind     11117..11133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11140..11158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(11197..11215)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11236..11252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11236..11252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11249..11271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11260..11276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11284..11290,11291..11308,11309..11314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11329..11350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11467..11484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11638..12226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11718..11737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12397..13188,13189..13257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13020..13039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13258..13362)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13388..13843)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13525..13546)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13985..14001
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        14008..14026
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atttaaatag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
       61 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      481 gatcaaggcg agttaggtac cgacgtgtcc caacaaccac tgctgcagcc agtacgggca
      541 ctgcggcttc ggagccgagt actgcggcgc cggctgccag ggcggcccct gccgcgccga
      601 catcaagtgc ggcagccagt ccggcggcaa gctatgcccg aacaacctct gctgcagcca
      661 gtggggattc tgcggcctag gttccgagtt ctgcggcggt ggctgccaga gcggtgcttg
      721 cagcaccgac aagccgtgcg gcaaggacgc cggcggcagg gtttgcacta acaactactg
      781 ttgtagcaag tggggatcct gtggcatgaa gctatcgata gcttccctta attgagaagt
      841 tcctattccg aagttcctat tcttcaaaag gtataggaac ttctgcagga atctagttat
      901 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      961 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     1021 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     1081 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1141 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1201 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     1261 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     1321 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
     1381 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
     1441 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
     1501 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga gtcgctgcga cgctgccttc
     1561 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1621 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1681 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
     1741 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     1801 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1861 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     1921 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     1981 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
     2041 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     2101 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     2161 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     2221 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     2281 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     2341 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     2401 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     2461 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     2521 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     2581 tgctgtctca tcattttggc aaagaattga tttgataccg cgggcccggg tcgacggtat
     2641 cgataagcag cttgatgatc tgtgacatgg cggatcccgt cgttttacaa cgtcgtgact
     2701 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2761 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2821 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
     2881 gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg
     2941 atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca
     3001 cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg
     3061 aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg
     3121 ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat
     3181 ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt
     3241 atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc
     3301 ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca
     3361 gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg
     3421 taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg
     3481 gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg
     3541 aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc
     3601 acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc
     3661 ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc
     3721 gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata
     3781 tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc
     3841 cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg
     3901 aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga
     3961 tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct
     4021 ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca
     4081 aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca
     4141 ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc
     4201 cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt
     4261 gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt
     4321 ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga
     4381 ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc
     4441 cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag
     4501 cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca
     4561 tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg
     4621 tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag
     4681 gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc
     4741 tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg
     4801 cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg
     4861 ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt
     4921 ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac
     4981 tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa
     5041 gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt
     5101 accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga
     5161 ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct
     5221 accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata
     5281 caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa
     5341 actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg
     5401 accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg
     5461 gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc
     5521 agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc
     5581 tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg
     5641 acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt cgctaccatt
     5701 accagttggt ctggtgtcag gggatccccc gggctgcagc caatatggga tcggccattg
     5761 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5821 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5881 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5941 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6001 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6061 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6121 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6181 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6241 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg
     6301 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6361 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6421 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6481 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6541 tcttctgagg ggatcaattc tctaggcttg ggatctttgt gaaggaacct tacttctgtg
     6601 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     6661 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattcaca
     6721 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc
     6781 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     6841 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     6901 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcata atcagccata
     7021 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     7081 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     7141 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7201 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcataatc agccatacca
     7261 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     7321 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     7381 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7441 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccactagttc tagctagtct
     7501 aggtcgatgc aggataactt cgtatagcat acattatacg aagttataga tcttgggtac
     7561 cgagctcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     7621 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     7681 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     7741 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     7801 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     7861 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     7921 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     7981 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     8041 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcgacc cgcttaacag
     8101 cgtcaacagc gtgccgcaga tcttggtggc gtgaaactcc cgcacctctt cggcaagcgc
     8161 cttgtagaag cgcgtatggc ttcgtacccc tgccatcaac acgcgtctgc gttcgaccag
     8221 gctgcgcgtt ctcgcggcca tagcaaccga cgtacggcgt tgcgccctcg ccggcagcaa
     8281 gaagccacgg aagtccgcct ggagcagaaa atgcccacgc tactgcgggt ttatatagac
     8341 ggtcctcacg ggatggggaa aaccaccacc acgcaactgc tggtggccct gggttcgcgc
     8401 gacgatatcg tctacgtacc cgagccgatg acttactggc aggtgctggg ggcttccgag
     8461 acaatcgcga acatctacac cacacaacac cgcctcgacc agggtgagat atcggccggg
     8521 gacgcggcgg tggtaatgac aagcgcccag ataacaatgg gcatgcctta tgccgtgacc
     8581 gacgccgttc tggctcctca tatcgggggg gaggctggga gctcacatgc cccgcccccg
     8641 gccctcaccc tcatcttcga ccgccatccc atcgccgccc tcctgtgcta cccggccgcg
     8701 cgatacctta tgggcagcat gaccccccag gccgtgctgg cgttcgtggc cctcatcccg
     8761 ccgaccttgc ccggcacaaa catcgtgttg ggggcccttc cggaggacag acacatcgac
     8821 cgcctggcca aacgccagcg ccccggcgag cggcttgacc tggctatgct ggccgcgatt
     8881 cgccgcgttt acgggctgct tgccaatacg gtgcggtatc tgcagggcgg cgggtcgtgg
     8941 cgggaggatt ggggacagct ttcggggacg gccgtgccgc cccagggtgc cgagccccag
     9001 agcaacgcgg gcccacgacc ccatatcggg gacacgttat ttaccctgtt tcgggccccc
     9061 gagttgctgg cccccaacgg cgacctgtac aacgtgtttg cctgggcctt ggacgtcttg
     9121 gccaaacgcc tccgtcccat gcacgtcttt atcctggatt acgaccaatc gcccgccggc
     9181 tgccgggacg ccctgctgca acttacctcc gggatggtcc agacccacgt caccaccccc
     9241 ggctccatac cgacgatctg cgacctggcg cgcacgtttg cccgggagat gggggaggct
     9301 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     9361 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     9421 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     9481 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     9541 gggcggcaag ccctgccata gccacgggcc ccgtgggtta gggacggggt cccccatggg
     9601 gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga
     9661 ctggactgag cagacagacc catggttttt ggatggcctg ggcatggacc gcatgtactg
     9721 gcgcgacacg aacaccgggc gtctgtggct gccaaacacc cccgaccccc aaaaaccacc
     9781 gcgcggattt ctggcgccgc cggacgaact aaacctgact acggcatctc tgccccttct
     9841 tcgctggtac gaggagcgct tttgttttgt attggtcacc acggccgagt ttccgcggga
     9901 ccccggccag gacctgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat
     9961 ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa
    10021 tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt
    10081 actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa
    10141 acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata
    10201 gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg
    10261 gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat
    10321 acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaagct taattctaga
    10381 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
    10441 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taaattgggc
    10501 gcggaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt cctagttcta
    10561 gagcggccgc tcgacatggt agccgccgtt gttgttattg tagttgctac tgttatttct
    10621 ggcacttctt ggttttcctc ttaagtgagg aggaacataa ccattctcgt tgttgtcgtt
    10681 gatgcttaaa ttttgcactt gttcgctcag ttcagccata atatgaaatg cttttcttgt
    10741 tgttcttacg gaataccact tgccacctat caccgagctc ggtgccgtaa agcactaaat
    10801 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
    10861 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
    10921 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
    10981 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    11041 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11101 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
    11161 gaattgggta ggcgcgccga gctccagctt ttgttccctt tagtgagggt taatttcgag
    11221 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    11281 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    11341 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    11401 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    11461 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    11521 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    11581 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    11641 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11701 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11761 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11821 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11881 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11941 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    12001 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    12061 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    12121 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    12181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    12241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    12301 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    12361 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    12421 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    12481 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    12541 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    12601 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12661 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12721 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12781 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12841 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12901 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12961 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    13021 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    13081 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    13141 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    13201 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    13261 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    13321 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    13381 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
    13441 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
    13501 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
    13561 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
    13621 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    13681 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    13741 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    13801 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
    13861 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    13921 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    13981 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attgggtacc
//
