LOCUS       sequence-19039-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Jiang Z, Georgel P, Li C, Choe J, Crozat K, Rutschmann S, Du X, 
            Bigby T, Mudd S, Sovath S, Wilson IA, Olson A, Beutler B
  TITLE     Details of Toll-like receptor:adapter interaction revealed by 
            germ-line mutagenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):10961-6.
  PUBMED    16832055
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):10961-6."
COMMENT     Sequence Label: pCMV-HA-MyD88-P200H
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        232..250
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     232..249
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     263..282
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             337..426
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 cgtttagtga accgtcagat tcgcctggag acgccatcca cgctgttttg acctccatag
       61 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
      121 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
      181 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
      241 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
      301 ctgcacctcg gttctatcga tatgggccgc atcttttacc catacgatgt tcctgactat
      361 gcgggctatc cctatgacgt cccggactat gcaggatcct atccatatga cgttccagat
      421 tacgctgctc agcgcgaatt cgcggccgca gctgcaggag gtcccggcgc ggggtctgcg
      481 gccccggtct cctccacatc ctcccttccc ctggctgctc tcaacatgcg agtgcggcgc
      541 cgcctgtctc tgttcttgaa cgtgcggaca caggtggcgg ccgactggac cgcgctggcg
      601 gaggagatgg actttgagta cttggagatc cggcaactgg agacacaagc ggaccccact
      661 ggcaggctgc tggacgcctg gcagggacgc cctggcgcct ctgtaggccg actgctcgag
      721 ctgctttaca agctgggccg cgacgacgtg ctgctggagc t
//
