LOCUS       sequence-190390-       13261 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 13261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pMADM-beta
FEATURES             Location/Qualifiers
     source          1..13261
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     322..344
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     406..425
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             522..3563
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             3588..4391
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3651..3670)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4261..4280
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          4491..4556
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    4716..4850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4953..5087
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5190..5324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5357..5390)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5421..5920
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5456..5479)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5853..5872
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     5'UTR           5962..6001
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             6019..7149
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(7147..7166)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7191..7239
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    8347..8394
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(8613..9083)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWSSARLPNSPYSESYYNSLAVVLQRRD
                     WENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVT
                     RSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHRAR"
     primer_bind     8960..8976
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8983..9001
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9040..9058)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9079..9095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9079..9095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9092..9114)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9103..9119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9127..9133,9134..9151,9152..9157))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9172..9193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9310..9327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9481..10069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9561..9580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10240..11031,11032..11100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10863..10882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11101..11205)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11231..11686)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11368..11389)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11828..11844
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11851..11869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        12837..13140
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
       61 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
      121 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
      181 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
      241 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
      301 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
      361 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
      421 ttgtgctgtc tcatcatttt ggcaaagaat tgatttgata ccgcgggccc gggtcgacgg
      481 tatcgataag cagcttgatg atctgtgaca tggcggatcc cgtcgtttta caacgtcgtg
      541 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      601 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      661 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
      721 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
      781 acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc
      841 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
      901 aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca
      961 acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg
     1021 catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg agtgacggca
     1081 gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc
     1141 tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt
     1201 tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac
     1261 gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg
     1321 gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg
     1381 tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac
     1441 tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg
     1501 tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta
     1561 accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg
     1621 atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc
     1681 atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata
     1741 ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg
     1801 cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca
     1861 tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga
     1921 tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg
     1981 ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg
     2041 tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc
     2101 tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg
     2161 cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc
     2221 tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata
     2281 cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc
     2341 cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa
     2401 ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga
     2461 tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac
     2521 aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct
     2581 ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca
     2641 gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc
     2701 acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc
     2761 gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac
     2821 aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg
     2881 taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc
     2941 attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc
     3001 tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa
     3061 cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg
     3121 atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg
     3181 taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt
     3241 ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa
     3301 acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact
     3361 tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc
     3421 tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg
     3481 gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc
     3541 attaccagtt ggtctggtgt caggggatcc cccgggctgc agccaatatg ggatcggcca
     3601 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3661 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3721 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     3781 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3841 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3901 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3961 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4021 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4081 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     4141 atgatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4201 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4261 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4321 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4381 agttcttctg aggggatcaa ttctctaggc ttgggatctt tgtgaaggaa ccttacttct
     4441 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     4501 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     4561 acagtcccaa ggctcatttc aggcccctca gtcctcacag tctgttcatg atcataatca
     4621 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     4681 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4741 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4801 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ataatcagcc
     4861 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     4921 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     4981 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5041 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcata atcagccata
     5101 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5161 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5221 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5281 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccactag ttctagctag
     5341 tctaggtcga tgcaggataa cttcgtatag catacattat acgaagttat agatcttggg
     5401 taccgagctc gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     5461 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     5521 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     5581 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     5641 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     5701 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     5761 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     5821 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     5881 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcg acccgcttaa
     5941 cagcgtcaac agcgtgccgc agatcttggt ggcgtgaaac tcccgcacct cttcggcaag
     6001 cgccttgtag aagcgcgtat ggcttcgtac ccctgccatc aacacgcgtc tgcgttcgac
     6061 caggctgcgc gttctcgcgg ccatagcaac cgacgtacgg cgttgcgccc tcgccggcag
     6121 caagaagcca cggaagtccg cctggagcag aaaatgccca cgctactgcg ggtttatata
     6181 gacggtcctc acgggatggg gaaaaccacc accacgcaac tgctggtggc cctgggttcg
     6241 cgcgacgata tcgtctacgt acccgagccg atgacttact ggcaggtgct gggggcttcc
     6301 gagacaatcg cgaacatcta caccacacaa caccgcctcg accagggtga gatatcggcc
     6361 ggggacgcgg cggtggtaat gacaagcgcc cagataacaa tgggcatgcc ttatgccgtg
     6421 accgacgccg ttctggctcc tcatatcggg ggggaggctg ggagctcaca tgccccgccc
     6481 ccggccctca ccctcatctt cgaccgccat cccatcgccg ccctcctgtg ctacccggcc
     6541 gcgcgatacc ttatgggcag catgaccccc caggccgtgc tggcgttcgt ggccctcatc
     6601 ccgccgacct tgcccggcac aaacatcgtg ttgggggccc ttccggagga cagacacatc
     6661 gaccgcctgg ccaaacgcca gcgccccggc gagcggcttg acctggctat gctggccgcg
     6721 attcgccgcg tttacgggct gcttgccaat acggtgcggt atctgcaggg cggcgggtcg
     6781 tggcgggagg attggggaca gctttcgggg acggccgtgc cgccccaggg tgccgagccc
     6841 cagagcaacg cgggcccacg accccatatc ggggacacgt tatttaccct gtttcgggcc
     6901 cccgagttgc tggcccccaa cggcgacctg tacaacgtgt ttgcctgggc cttggacgtc
     6961 ttggccaaac gcctccgtcc catgcacgtc tttatcctgg attacgacca atcgcccgcc
     7021 ggctgccggg acgccctgct gcaacttacc tccgggatgg tccagaccca cgtcaccacc
     7081 cccggctcca taccgacgat ctgcgacctg gcgcgcacgt ttgcccggga gatgggggag
     7141 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     7201 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7261 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7321 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7381 tcggggcggc aagccctgcc atagccacgg gccccgtggg ttagggacgg ggtcccccat
     7441 ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca
     7501 cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta
     7561 ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc
     7621 accgcgcgga tttctggcgc cgccggacga actaaacctg actacggcat ctctgcccct
     7681 tcttcgctgg tacgaggagc gcttttgttt tgtattggtc accacggccg agtttccgcg
     7741 ggaccccggc caggacctgc agaaattgat gatctattaa acaataaaga tgtccactaa
     7801 aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt
     7861 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc
     7921 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatcataatt
     7981 taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct
     8041 atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact
     8101 gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca
     8161 catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa gcttaattct
     8221 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     8281 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaaattg
     8341 ggcgcggaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcctagtt
     8401 ctagagcggc cgctcgacat ggtagccgcc gttgttgtta ttgtagttgc tactgttatt
     8461 tctggcactt cttggttttc ctcttaagtg aggaggaaca taaccattct cgttgttgtc
     8521 gttgatgctt aaattttgca cttgttcgct cagttcagcc ataatatgaa atgcttttct
     8581 tgttgttctt acggaatacc acttgccacc tatcaccgag ctcggtgccg taaagcacta
     8641 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     8701 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     8761 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     8821 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     8881 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     8941 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     9001 ggcgaattgg gtaggcgcgc cgagctccag cttttgttcc ctttagtgag ggttaatttc
     9061 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11221 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
    11281 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
    11341 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
    11401 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
    11461 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
    11521 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
    11581 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
    11641 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
    11701 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11761 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    11821 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt
    11881 accatttaaa tagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    11941 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    12001 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    12061 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    12121 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    12181 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    12241 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    12301 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    12361 aacgatcaag gcgagttagg taccgacgtg tcccaacaac cactgctgca gccagtacgg
    12421 gcactgcggc ttcggagccg agtactgcgg cgccggctgc cagggcggcc cctgccgcgc
    12481 cgacatcaag tgcggcagcc agtccggcgg caagctatgc ccgaacaacc tctgctgcag
    12541 ccagtgggga ttctgcggcc taggttccga gttctgcggc ggtggctgcc agagcggtgc
    12601 ttgcagcacc gacaagccgt gcggcaagga cgccggcggc agggtttgca ctaacaacta
    12661 ctgttgtagc aagtggggat cctgtggcat gaagctatcg atagcttccc ttaattgaga
    12721 agttcctatt ccgaagttcc tattcttcaa aaggtatagg aacttctgca ggaatctagt
    12781 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    12841 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    12901 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    12961 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    13021 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    13081 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
    13141 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
    13201 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
    13261 g
//
