LOCUS       sequence-190396-       10651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CCNT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: CCNT1_pLX307
FEATURES             Location/Qualifiers
     source          1..10651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3502..3526)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3534..3575
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3686..4274
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3739..3759)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4157..4168)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4346..4579
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4657..4778
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4694..4713)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4748..4767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4818..4953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4973..4992)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4974..4992)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5002..5019)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5002..5018)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5011..5033)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5160..5615
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5247..5266)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5457..5478
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5641..5745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5746..5814,5815..6606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5964..5983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6777..7365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7266..7285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7519..7536
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7653..7674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7689..7694,7695..7712,7713..7719)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7727..7743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7732..7754
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7751..7767
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7751..7767
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7786..7806
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7788..7806
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7834..8060
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8061..8241
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8288..8413
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8906..9139
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9324..9368
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9599..9809
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9621..9817
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9668..9803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9849..10451
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9849..9868)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10345..10365
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10494..10611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggagggaga gaggaagaac aacaacaaac ggtggtattt cactcgagaa cagctggaaa
     1381 atagcccatc ccgtcgtttt ggcgtggacc cagataaaga actttcttat cgccagcagg
     1441 cggccaatct gcttcaggac atggggcagc gtcttaacgt ctcacaattg actatcaaca
     1501 ctgctatagt atacatgcat cgattctaca tgattcagtc cttcacacag ttccctggaa
     1561 attctgtggc tccagcagcc ttgtttctag cagctaaagt ggaggagcag cccaaaaaat
     1621 tggaacatgt catcaaggta gcacatactt gtctccatcc tcaggaatcc cttcctgata
     1681 ctagaagtga ggcttatttg caacaagttc aagatctggt cattttagaa agcataattt
     1741 tgcagacttt aggctttgaa ctaacaattg atcacccaca tactcatgta gtaaagtgca
     1801 ctcaacttgt tcgagcaagc aaggacttag cacagacttc ttacttcatg gcaaccaaca
     1861 gcctgcattt gaccacattt agcctgcagt acacacctcc tgtggtggcc tgtgtctgca
     1921 ttcacctggc ttgcaagtgg tccaattggg agatcccagt ctcaactgac gggaagcact
     1981 ggtgggagta tgttgacgcc actgtgacct tggaactttt agatgaactg acacatgagt
     2041 ttctacagat tttggagaaa actcccaaca ggctcaaacg catttggaat tggagggcat
     2101 gcgaggctgc caagaaaaca aaagcagatg accgaggaac agatgaaaag acttcagagc
     2161 agacaatcct caatatgatt tcccagagct cttcagacac aaccattgca ggtttaatga
     2221 gcatgtcaac ttctaccaca agtgcagtgc cttccctgcc agtctccgaa gagtcatcca
     2281 gcaacttaac cagtgtggag atgttgccgg gcaagcgttg gctgtcctcc caaccttctt
     2341 tcaaactaga acctactcag ggtcatcgga ctagtgagaa tttagcactt acaggagttg
     2401 atcattcctt accacaggat ggttcaaatg catttatttc ccagaagcag aatagtaaga
     2461 gtgtgccatc agctaaagtg tcactgaaag aataccgcgc gaagcatgca gaagaattgg
     2521 ctgcccagaa gaggcaactg gagaacatgg aagccaatgt gaagtcacaa tatgcatatg
     2581 ctgcccagaa tctcctttct catcatgata gccattcttc agtcattcta aaaatgccca
     2641 tagagggttc agaaaacccc gagcggcctt ttctggaaaa ggctgacaaa acagctctca
     2701 aaatgagaat cccagtggca ggtggagata aagctgcgtc ttcaaaacca gaggagataa
     2761 aaatgcgcat aaaagtccat gctgcagctg ataagcacaa ttctgtagag gacagtgtta
     2821 caaagagccg agagcacaaa gaaaagcaca agactcaccc atctaatcat catcgtcatc
     2881 ataatcacca ctcacacaag cactctcatt cccaacttcc agttggtact gggaacaaac
     2941 gtcctggtga tccaaaacat agtagccaga caagcaactt agcacataaa acctatagct
     3001 tgtctagttc ttttccctct tccagttcta ctcgtaaaag gggaccctct gaagagactg
     3061 gaggggctgt gtttgatcat ccagccaaga ttgccaagag tactaaatcc tcttccctaa
     3121 atttctcctt cccttcactt cctacaatgg gtcagatgcc tgggcatagc tcagacacaa
     3181 gtggcctttc cttttcacag cccagctgta aaactcgtgt ccctcattcg aaactggata
     3241 aagggcccac tggggccaat ggtcacaaca cgacccagac aatagactat caagacactg
     3301 tgaatatgct tcactccctg ctcagtgccc agggtgttca gcccactcag cctactgcat
     3361 ttgaatttgt tcgtccttat agtgactatc tgaatcctcg gtctggtgga atctcctcga
     3421 gatctggcaa tacagacaaa ccccggccac catctctgcc atcagaacct cctccaccac
     3481 ttccacccct tcctaagaga cccccaactt tcttgtacaa agtggttgat atcggtaagc
     3541 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt ccgtaacttg
     3601 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatact aaaacccttc
     3661 ctcggaaccg acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     3721 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3781 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3841 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3901 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3961 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4021 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4081 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4141 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4201 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4261 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     4321 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     4381 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4441 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4501 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4561 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     4621 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     4681 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4741 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     4801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4921 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     4981 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5041 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5101 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     5161 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5221 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5281 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5341 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5401 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5461 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5521 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5581 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5641 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5701 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5761 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5821 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5881 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5941 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6001 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6061 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6121 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6181 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6241 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6301 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6361 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6421 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6481 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6541 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6601 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6661 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6721 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6781 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6841 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6901 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6961 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7021 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7081 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7141 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7201 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7261 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7321 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7381 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7441 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7501 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7561 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7621 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7681 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7741 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7801 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7861 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     7921 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     7981 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     8041 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     8101 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8161 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8221 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8281 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8341 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8401 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8461 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8521 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8581 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8641 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8701 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8761 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8821 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8881 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8941 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9001 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9061 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9121 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9181 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9241 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9301 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9361 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9421 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9481 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     9541 ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9601 aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac
     9661 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9721 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9781 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg
     9841 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     9901 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     9961 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
    10021 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
    10081 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
    10141 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
    10201 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
    10261 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
    10321 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
    10381 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
    10441 gtgccagtta acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa
    10501 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    10561 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    10621 tacagggaca gcagagatcc actttggcgc c
//
