LOCUS       sequence-190398-        9409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of DIMT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: DIMT1_pLX307
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2260..2284)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2292..2333
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2444..3032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2497..2517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2915..2926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3104..3337
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3415..3536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3452..3471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3506..3525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3576..3711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3731..3750)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3732..3750)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3760..3777)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3760..3776)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3769..3791)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3918..4373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4005..4024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4215..4236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4399..4503
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4504..4572,4573..5364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4722..4741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5535..6123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6024..6043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6277..6294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6411..6432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6447..6452,6453..6470,6471..6477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6485..6501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6490..6512
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6509..6525
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6509..6525
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6544..6564
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6546..6564
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6592..6818
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6819..6999
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7046..7171
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7664..7897
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8082..8126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8357..8567
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8379..8575
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8426..8561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8607..9209
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8607..8626)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9103..9123
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9252..9369
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgccgaaggt caagtcgggg gccatcggcc gccgccgcgg gcggcaggag cagcgccggg
     1381 agctgaagag cgctggagga ctcatgttca acacggggat tgggcagcac attttgaaaa
     1441 atcctctcat tattaacagc attatcgata aggctgcctt aagaccaact gatgtagtgc
     1501 tggaagttgg acctggaact ggcaacatga ctgtaaagtt gttagaaaag gcaaaaaagg
     1561 ttgttgcttg tgaacttgac ccaaggctag tagctgaact tcacaaaaga gttcagggca
     1621 cgcctgtggc cagcaaactt caagtactgg tgggtgatgt gctgaaaaca gatttgccat
     1681 tctttgatac ttgtgtggca aatttgcctt atcagatctc ttcgcctttt gtcttcaagc
     1741 tgttgctaca tcgacctttt ttcaggtgtg ctatacttat gtttcaaaga gaatttgccc
     1801 tccgactggt tgcaaaacct ggagataagt tatactgcag actctcaatt aatacacagc
     1861 tgttggcacg tgtggaccat ctaatgaaag tgggaaagaa taacttcaga ccaccgccca
     1921 aggtggaatc cagtgttgta aggatagaac ctaagaatcc accaccaccc atcaattttc
     1981 aggaatggga tggtctagta aggataacct ttgttaggaa aaacaagaca ctctctgctg
     2041 catttaaatc aagtgcagtg caacaactct tggaaaaaaa ctacagaatt cactgttcag
     2101 tccataatat tataatacca gaagatttca gcatagcaga taaaatacag caaatcctaa
     2161 ccagcacagg ttttagtgac aaacgggccc gttccatgga catagatgac ttcatcagat
     2221 tgctacatgg attcaacgca gaaggtattc atttttccta cccaactttc ttgtacaaag
     2281 tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     2341 gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2401 acgattgcta agttgagcaa cgaaagatac gcgttaagtc gacaatcaac ctctggatta
     2461 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2521 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2581 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2641 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2701 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2761 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2821 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     2881 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2941 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3001 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     3061 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     3121 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     3181 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3241 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3301 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     3361 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     3421 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3481 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3541 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3661 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     3721 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     3781 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3841 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     3901 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3961 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4021 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4081 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4141 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4201 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4261 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4321 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     4381 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4441 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4501 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4561 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4621 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4681 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4741 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4801 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4861 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4921 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4981 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5041 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5101 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5161 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5221 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5281 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5341 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5641 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6181 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6241 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6301 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6361 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6421 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6481 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6541 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     6601 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     6661 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     6721 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     6781 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     6841 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6901 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6961 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7021 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7081 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7141 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7201 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7261 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7321 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7381 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     7441 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     7501 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     7561 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7621 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7681 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     7741 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     7801 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     7861 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     7921 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     7981 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8041 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8101 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     8161 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     8221 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     8281 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
     8341 gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac
     8401 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8461 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8521 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8581 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     8641 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     8701 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     8761 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     8821 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     8881 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     8941 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     9001 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     9061 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     9121 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     9181 gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta
     9241 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     9301 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     9361 caaaattttc gggtttatta cagggacagc agagatccac tttggcgcc
//
