LOCUS       sequence-190401-       10945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EMR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: EMR2_pLX307
FEATURES             Location/Qualifiers
     source          1..10945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3796..3820)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3828..3869
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3980..4568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4033..4053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4451..4462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4640..4873
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4951..5072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4988..5007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5042..5061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5112..5247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5267..5286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5268..5286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5296..5313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5296..5312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5305..5327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5454..5909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5541..5560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5751..5772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5935..6039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6040..6108,6109..6900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6258..6277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7071..7659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7560..7579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7813..7830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7947..7968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7983..7988,7989..8006,8007..8013)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8021..8037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8026..8048
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8045..8061
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8045..8061
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8080..8100
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8082..8100
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8128..8354
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8355..8535
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8582..8707
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9200..9433
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9618..9662
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9893..10103
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9915..10111
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9962..10097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10143..10745
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10143..10162)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10639..10659
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10788..10905
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagcaggct
     1321 tcaccatggg aggccgcgtc tttctcgtct ttctcgcatt ctgtgtctgg ctgactctgc
     1381 cgggagctga aacccaggac tccaggggct gtgcccggtg gtgccctcag gactcctcgt
     1441 gtgtcaatgc caccgcctgt cgctgcaatc cagggttcag ctctttttct gagatcatca
     1501 ccacccccat ggagacttgt gacgacatca acgagtgtgc aacactgtcg aaagtgtcat
     1561 gcggaaaatt ctcggactgc tggaacacag aggggagcta cgactgcgtg tgcagcccag
     1621 gatatgagcc tgtttctggg gcaaaaacat tcaagaatga gagcgagaac acgtgtcaag
     1681 atgtggacga atgtcagcag aacccaaggc tctgtaaaag ctacggcacc tgcgtcaaca
     1741 cccttggcag ctatacctgc cagtgcctgc ctggcttcaa gttcatacct gaggatccga
     1801 aggtctgcac agatgtgaat gaatgcacct ccggacaaaa cccatgccac agctccaccc
     1861 actgcctcaa caacgtgggc agctatcagt gccgctgccg cccgggctgg caaccgattc
     1921 cggggtcccc caatggccca aacaataccg tctgtgaaga tgtggacgag tgcagctccg
     1981 ggcagcatca gtgtgacagc tccaccgtct gcttcaacac cgtgggttca tacagctgcc
     2041 gctgccgccc aggctggaag cccagacacg gaatcccgaa taaccaaaag gacactgtct
     2101 gtgaagatat gactttctcc acctggaccc cgccccctgg agtccacagc cagacgcttt
     2161 cccgattctt cgacaaagtc caggacctgg gcagagacta caagccaggc ttggccaata
     2221 acaccatcca gagcatctta caggcgctgg atgagctgct ggaggtccct ggggacctgg
     2281 agaccctgcc ccgcttacag cagcactgtg tggccagtca cctgctggat ggcctagagg
     2341 atgtcctcag aggcctgagc aagaaccttt ccaatgggct gttgaacttc agttatcctg
     2401 caggcacaga attgtccctg gaggtgcaga agcaagtaga caggagtgtc accttgagac
     2461 agaatcaggc agtgatgcag ctcgactgga atcaggcaca gaaatctggt gacccaggcc
     2521 cttctgtggt gggccttgtc tccattccag ggatgggcaa gttgctggct gaggcccctc
     2581 tggtcctgga acctgagaag cagatgcttc tgcatgagac acaccagggc ttgctgcagg
     2641 acggctcccc catcctgctc tcagatgtga tctctgcctt tctgagcaac aacgacaccc
     2701 aaaacctcag ctccccagtt accttcacct tctcccaccg ttcagtgatc ccgagacaga
     2761 aggtgctctg tgtcttctgg gagcatggcc agaatggatg tggtcactgg gccaccacag
     2821 gctgcagcac aataggcacc agagacacca gcaccatctg ccgttgcacc cacctgagca
     2881 gctttgccgt cctcatggcc cactacgatg tgcaggagga ggatcccgtg ctgactgtca
     2941 tcacctacat ggggctgagc gtctctctgc tgtgcctcct cctggcggcc ctcacttttc
     3001 tcctgtgtaa agccatccag aacaccagca cctcactgca tctgcagctc tcgctctgcc
     3061 tcttcctggc ccacctcctc ttcctcgtgg caattgatca aaccggacac aaggtgctgt
     3121 gctccatcat cgccggtacc ttgcactatc tctacctggc caccttgacc tggatgctgc
     3181 tggaggccct gtacctcttc ctcactgcac ggaacctgac ggtggtcaac tactcaagca
     3241 tcaacagatt catgaagaag ctcatgttcc ctgtgggcta cggagtccca gctgtgacag
     3301 tggccatttc tgcagcctcc aggcctcacc tttatggaac accttcccgc tgctggctcc
     3361 aaccagaaaa gggatttata tggggcttcc ttggacctgt ctgcgccatc ttctctgtga
     3421 atttagttct ctttctggtg actctctgga ttttgaaaaa cagactctcc tccctcaata
     3481 gtgaagtgtc caccctccgg aacacaagga tgctggcatt taaagcgaca gctcagctgt
     3541 tcatcctggg ctgcacgtgg tgtctgggca tcttgcaggt gggtccggct gcccgggtca
     3601 tggcctacct cttcaccatc atcaacagcc tgcagggtgt cttcatcttc ctggtgtact
     3661 gcctcctcag ccagcaggtc cgggagcaat atgggaaatg gtccaaaggg atcaggaaat
     3721 tgaaaactga gtctgagatg cacacactct ccagcagtgc taaggctgac acctccaaac
     3781 ccagcacggt taacgaccca gctttcttgt acaaagtggt tgatatcggt aagcctatcc
     3841 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa cttgaaagta
     3901 tttcgatttc ttggctttat atatcttgtg gaaaggacga acctgcattt aatcacactc
     3961 aaagacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4021 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4081 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4141 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4201 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4261 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4321 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4381 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4441 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4501 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4561 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4621 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     4681 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4741 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4801 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4861 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     4921 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5041 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     5101 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5161 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5221 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     5281 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5341 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5401 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     5461 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5521 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5581 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5641 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5701 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5761 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5821 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5881 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5941 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6001 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6061 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6121 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6181 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6241 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6301 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6361 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6421 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6481 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6541 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6601 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6661 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6721 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6781 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6841 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6901 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6961 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7021 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7081 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7141 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7201 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7261 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7321 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7381 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7441 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7501 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7561 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7621 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7681 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7741 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7801 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7921 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7981 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8041 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     8101 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     8161 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     8221 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     8281 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     8341 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8401 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8461 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8521 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8581 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8641 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8701 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8761 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8821 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8881 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8941 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     9001 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     9061 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9121 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     9181 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     9241 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     9301 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     9361 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9421 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9481 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     9541 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9601 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     9661 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     9721 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     9781 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc
     9841 attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9901 aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9961 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    10021 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    10081 aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta
    10141 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
    10201 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
    10261 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
    10321 acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg
    10381 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
    10441 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
    10501 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
    10561 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
    10621 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
    10681 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca
    10741 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
    10801 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    10861 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
    10921 gacagcagag atccactttg gcgcc
//
