LOCUS       sequence-190405-       11617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZNF217.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 11617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: ZNF217_pLX307
FEATURES             Location/Qualifiers
     source          1..11617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4468..4492)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4500..4541
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4652..5240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4705..4725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5123..5134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5312..5545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5623..5744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5660..5679)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5714..5733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5784..5919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5939..5958)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5940..5958)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5968..5985)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5968..5984)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5977..5999)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6126..6581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6213..6232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6423..6444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6607..6711
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6712..6780,6781..7572)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6930..6949)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7743..8331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8232..8251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8485..8502
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8619..8640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8655..8660,8661..8678,8679..8685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8693..8709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8698..8720
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8717..8733
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8717..8733
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8752..8772
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8754..8772
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8800..9026
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9027..9207
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9254..9379
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9872..10105
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10290..10334
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10565..10775
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10587..10783
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10634..10769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10815..11417
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10815..10834)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11311..11331
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11460..11577
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgcaatcgaa agtgacagga aacatgccaa ctcaatccct cttaatgtac atggatgggc
     1381 cagaagtgat tggcagctct cttggcagtc cgatggagat ggaggatgcc ttgtcaatga
     1441 aagggaccgc tgttgttcca ttccgagcta cacaagaaaa aaatgtcatc caaatcgagg
     1501 ggtatatgcc cttggattgc atgttctgca gccagacctt cacacattca gaagacctta
     1561 ataaacatgt cttaatgcaa caccggccta ccctctgtga accagcagtt cttcgggttg
     1621 aagcagagta tctcagtccg cttgataaaa gtcaagtgcg aacagaacct cccaaggaaa
     1681 agaattgcaa ggaaaatgaa tttagctgtg aggtatgtgg gcagacattt agagtcgctt
     1741 ttgatgttga gatccacatg agaacacaca aagattcttt cacttacggg tgtaacatgt
     1801 gcggaagaag attcaaggag ccttggtttc ttaaaaatca catgcggaca cataatggca
     1861 aatcgggggc cagaagcaaa ctgcagcaag gcttggagag tagtccagca acgatcaacg
     1921 aggtcgtcca ggtgcacgcg gccgagagca tctcctctcc ttacaaaatc tgcatggttt
     1981 gtggcttcct atttccaaat aaagaaagtc taattgagca ccgcaaggtg cacaccaaaa
     2041 aaactgcttt cggtaccagc agcgcgcaga cagactctcc acaaggagga atgccgtcct
     2101 cgagggagga cttcctgcag ttgttcaact tgagaccaaa atctcaccct gaaacgggga
     2161 agaagcctgt cagatgcatc cctcagctcg atccgttcac caccttccag gcttggcagc
     2221 tggctaccaa aggaaaagtt gccatttgcc aagaagtgaa ggaatcgggg caagaaggga
     2281 gcaccgacaa cgacgattcg agttccgaga aggagcttgg agaaacaaat aagggcagtt
     2341 gtgcaggcct ctcgcaagag aaagagaagt gcaaacactc ccacggcgaa gcgccctccg
     2401 tggacgcgga tcccaagtta cccagtagca aggagaagcc cactcactgc tccgagtgcg
     2461 gcaaagcttt cagaacctac caccagctgg tcttgcactc cagggtccac aagaaggacc
     2521 ggagggccgg cgcggagtcg cccaccatgt ctgtggacgg gaggcagccg gggacgtgtt
     2581 ctcctgacct cgccgcccct ctggatgaaa atggagccgt ggatcgaggg gaaggtggtt
     2641 ctgaagacgg atctgaggat gggcttcccg aaggaatcca tctggataaa aatgatgatg
     2701 gaggaaaaat aaaacatctt acatcttcaa gagagtgtag ttattgtgga aagtttttcc
     2761 gttcaaatta ttacctcaat attcatctca gaacgcatac aggtgaaaaa ccatacaaat
     2821 gtgaattttg tgaatatgct gcagcccaga agacatctct gaggtatcac ttggagagac
     2881 atcacaagga aaaacaaacc gatgttgctg ctgaagtcaa gaacgatggt aaaaatcagg
     2941 acactgaaga tgcactatta accgctgaca gtgcgcaaac caaaaatttg aaaagatttt
     3001 ttgatggtgc caaagatgtt acaggcagtc cacctgcaaa gcagcttaag gagatgcctt
     3061 ctgtttttca gaatgttctg ggcagcgctg tcctctcacc agcacacaaa gatactcagg
     3121 atttccataa aaatgcagct gatgacagtg ctgataaagt gaataaaaac cctacccctg
     3181 cttacctgga cctgttaaaa aagagatcag cagttgaaac tcaggcaaat aacctcatct
     3241 gtagaaccaa ggcggatgtt actcctcctc cggatggcag taccacccat aaccttgaag
     3301 ttagccccaa agagaagcaa acggagaccg cagctgactg cagatacagg ccaagtgtgg
     3361 attgtcacga aaaaccttta aatttatccg tgggggctct tcacaattgc ccggcaattt
     3421 ctttgagtaa aagtttgatt ccaagtatca cctgtccatt ttgtaccttc aagacatttt
     3481 atccagaagt tttaatgatg caccagagac tggagcataa atacaatcct gacgttcata
     3541 aaaactgtcg aaacaagtcc ttgcttagaa gtcgacgtac cggatgcccg ccagcgttgc
     3601 tgggaaaaga tgtgcctccc ctctctagtt tctgtaaacc caagcccaag tctgctttcc
     3661 cggcgcagtc caaatccctg ccatctgcga aggggaagca gagccctcct gggccaggca
     3721 aggcccctct gacttcaggg atagactcta gcactttagc cccaagtaac ctgaagtccc
     3781 acagaccaca gcagaatgtg ggggtccaag gggccgccac caggcaacag caatctgaga
     3841 tgtttcctaa aaccagtgtt tcccctgcac cggataagac aaaaagaccc gagacaaaat
     3901 tgaaacctct tccagtagct ccttctcagc ccaccctcgg cagcagtaac atcaatggtt
     3961 ccatcgacta ccccgccaag aacgacagcc cgtgggcacc tccgggaaga gactatttct
     4021 gtaatcggag tgccagcaat actgcagcag aatttggtga gccccttcca aaaagactga
     4081 agtccagcgt ggttgccctt gacgttgacc agcccggggc caattacaga agaggctatg
     4141 accttcccaa gtaccatatg gtcagaggca tcacatcact gttaccgcag gactgtgtgt
     4201 atccgtcgca ggcgctgcct cccaaaccaa ggttcctgag ctccagcgag gtcgattctc
     4261 caaatgtgct gactgttcag aagccctatg gtggctccgg gccactttac acttgtgtgc
     4321 ctgctggtag tccagcatcc agctcgacgt tagaaggaaa aaggcctgtg tcatatcaac
     4381 acttatctaa cagcatggca caaaagagaa actatgagaa ttttattggg aatgcacatt
     4441 atcgaccaaa tgacaaaaaa actccacccc caactttctt gtacaaagtg gttgatatcg
     4501 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt
     4561 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gatactaaaa
     4621 cccttcctcg gaaccgacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     4681 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4741 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4801 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4861 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4921 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4981 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5041 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5101 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5161 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5221 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     5281 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5341 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5401 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5461 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5521 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     5581 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5641 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5701 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5761 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5821 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5881 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     5941 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     6001 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     6061 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6121 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6541 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6601 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6661 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6721 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6781 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6841 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6901 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6961 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7021 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7081 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7141 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7201 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7261 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7321 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7381 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7441 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7501 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7561 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7621 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7681 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7741 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7801 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7861 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7921 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7981 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8041 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8101 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8161 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8221 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8281 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8341 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8401 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8461 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8521 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8581 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8641 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8701 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     8761 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     8821 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     8881 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     8941 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     9001 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     9061 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9121 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9181 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     9241 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     9301 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     9361 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     9421 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     9481 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     9541 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     9601 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     9661 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     9721 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9781 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9841 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9901 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9961 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10021 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10081 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10141 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10201 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10261 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10321 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10381 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10441 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10501 ggggacccat gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10561 cccagcaggc agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc
    10621 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10681 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    10741 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac
    10801 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
    10861 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
    10921 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
    10981 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct
    11041 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
    11101 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
    11161 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
    11221 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
    11281 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
    11341 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
    11401 agcccggtgc cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat
    11461 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    11521 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
    11581 gtttattaca gggacagcag agatccactt tggcgcc
//
