LOCUS       sequence-190406-        9082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of YAF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: YAF2_pLX307
FEATURES             Location/Qualifiers
     source          1..9082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1933..1957)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1965..2006
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2117..2705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2170..2190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2588..2599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2777..3010
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3088..3209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3125..3144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3179..3198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3249..3384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3404..3423)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3405..3423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3433..3450)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3433..3449)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3442..3464)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3591..4046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3678..3697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3888..3909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4072..4176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4177..4245,4246..5037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4395..4414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5208..5796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5697..5716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5950..5967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6084..6105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6120..6125,6126..6143,6144..6150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6158..6174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6163..6185
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6182..6198
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6182..6198
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6217..6237
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6219..6237
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6265..6491
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6492..6672
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6719..6844
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7337..7570
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7755..7799
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8030..8240
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8052..8248
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8099..8234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8280..8882
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8280..8299)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8776..8796
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8925..9042
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgggagacaa gaagagcccc accaggccga agcggcagcc gaagccgtcc tcggatgagg
     1381 gttactggga ctgtagcgtc tgcaccttcc ggaacagcgc cgaggccttc aagtgcatga
     1441 tgtgcgatgt gcggaagggc acctccaccc gatccacatt gtttgaagtt attgtgagtg
     1501 cctcgaggac caaggaaccg ttgaaatttc caatttcagg aaggaaacct cgacctgtct
     1561 cccagttggt tgcacagcag gttactcagc agtttgtgcc tcctacacag tcaaagaaag
     1621 agaaaaaaga taaagtagaa aaagaaaaaa gtgaaaagga aacaactagc aaaaagaata
     1681 gccataagaa aaccaggcca agattgaaaa atgtggatcg gagtagtgct cagcatttgg
     1741 aagttactgt tggagatctg acagtcatta ttacagactt taaggagaaa acaaagtcac
     1801 cgcctgcatc tagtgctgcc tctgcagatc aacacagtca aagcggctct agctctgata
     1861 acacagagag aggaatgtcc aggtcatctt cacccagagg agaagcctca tcattgaatg
     1921 gagaatctca ttacccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta
     1981 accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt
     2041 cgatttcttg gctttatata tcttgtggaa aggacgacgg tattactatg agtgcgcaac
     2101 cacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2161 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2221 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2281 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2341 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2401 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2461 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     2521 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     2581 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     2641 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     2701 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     2761 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     2821 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2881 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2941 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3001 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     3061 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     3121 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3181 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     3241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3301 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3361 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     3421 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3481 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3541 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     3601 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3661 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3721 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3781 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     3841 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3901 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     3961 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4021 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     4081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4141 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4201 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4261 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4321 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4381 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4441 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4501 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4561 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4621 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4681 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4741 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4801 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4861 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4921 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4981 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5041 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5341 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5881 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5941 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6001 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6061 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     6121 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     6181 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     6241 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     6301 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     6361 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     6421 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     6481 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6541 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6601 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6661 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     6721 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     6781 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     6841 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     6901 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     6961 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     7021 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     7081 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     7141 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     7201 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     7261 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     7321 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     7381 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     7441 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     7501 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     7561 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     7621 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     7681 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     7741 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     7801 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     7861 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     7921 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt
     7981 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8041 tatgcaaagc atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8161 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8221 cttttttgga ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca
     8281 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     8341 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     8401 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     8461 tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga
     8521 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     8581 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     8641 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     8701 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     8761 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     8821 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt
     8881 aacgaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     8941 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9001 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9061 agcagagatc cactttggcg cc
//
