LOCUS       sequence-190407-        9400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GPR35.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: GPR35_pLX307
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2251..2275)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2283..2324
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2435..3023
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2488..2508)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2906..2917)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3095..3328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3406..3527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3443..3462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3497..3516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3567..3702
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3722..3741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3723..3741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3751..3768)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3751..3767)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3760..3782)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3909..4364
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3996..4015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4206..4227
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4390..4494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4495..4563,4564..5355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5526..6114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6015..6034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6268..6285
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6402..6423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6438..6443,6444..6461,6462..6468)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6476..6492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6481..6503
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6500..6516
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6500..6516
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6535..6555
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6537..6555
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6583..6809
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6810..6990
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7037..7162
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7655..7888
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8073..8117
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8348..8558
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8370..8566
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8417..8552
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8598..9200
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8598..8617)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9094..9114
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9243..9360
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 ccatgaatgg cacctacaac acctgtggct ccagcgacct cacctggccc ccagcgatca
     1381 agctgggctt ctacgcctac ttgggcgtcc tgctggtgct aggcctgctg ctcaacagcc
     1441 tggcgctctg ggtgttctgc tgccgcatgc agcagtggac ggagacccgc atctacatga
     1501 ccaacctggc ggtggccgac ctctgcctgc tgtgcacctt gcccttcgtg ctgcactccc
     1561 tgcgagacac ctcagacacg ccgctgtgcc agctctccca gggcatctac ctgaccaaca
     1621 ggtacatgag catcagcctg gtcacggcca tcgccgtgga ccgctatgtg gccgtgcggc
     1681 acccgctgcg tgcccgcggg ctgcggtccc ccaggcaggc tgcggccgtg tgcgcggtcc
     1741 tctgggtgct ggtcatcggc tccctggtgg ctcgctggct cctggggatt caggagggcg
     1801 gcttctgctt caggagcacc cggcacaatt tcaactccat ggcgttcccg ctgctgggat
     1861 tctacctgcc cctggccgtg gtggtcttct gctccctgaa ggtggtgact gccctggccc
     1921 agaggccacc caccgacgtg gggcaggcag aggccacccg caaggctgcc cgcatggtct
     1981 gggccaacct cctggtgttc gtggtctgct tcctgcccct gcacgtgggg ctgacagtgc
     2041 gcctcgcagt gggctggaac gcctgtgccc tcctggagac gatccgtcgc gccctgtaca
     2101 taaccagcaa gctctcagat gccaactgct gcctggacgc catctgctac tactacatgg
     2161 ccaaggagtt ccaggaggcg tctgcactgg ccgtggctcc ccgtgctaag gcccacaaaa
     2221 gccaggactc tctgtgcgtg accctcgcct tgccaacttt cttgtacaaa gtggttgata
     2281 tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaactagtc
     2341 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaaatt
     2401 cgtcaaggtc tgccctcgca cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     2461 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2521 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2581 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2641 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2701 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2761 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2821 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2881 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2941 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3001 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     3061 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     3121 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     3181 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3241 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3301 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     3361 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     3421 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3481 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     3541 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3601 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3661 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     3721 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     3781 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3841 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3901 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3961 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4021 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4081 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4141 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4201 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4261 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4321 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     4381 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4441 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4501 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4561 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4621 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4681 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4741 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4801 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4861 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4921 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4981 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5041 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5101 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5161 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5221 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5281 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5341 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5401 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5461 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5521 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5581 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5641 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5701 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5761 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5821 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5881 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5941 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6001 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6061 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6121 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6181 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6241 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6301 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6361 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6421 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6481 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     6541 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     6601 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     6661 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     6721 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     6781 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     6841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6961 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     7021 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     7081 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     7141 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     7201 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     7261 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     7321 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     7381 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     7441 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     7501 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     7561 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     7621 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     7681 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     7741 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     7801 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7861 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7921 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7981 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     8041 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     8101 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     8161 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     8221 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     8281 cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8341 ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc
     8401 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8461 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8521 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg
     8581 taccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     8641 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     8701 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     8761 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg
     8821 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     8881 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     8941 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     9001 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     9061 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     9121 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     9181 gcaagcccgg tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac
     9241 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     9301 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9361 cgggtttatt acagggacag cagagatcca ctttggcgcc
//
