LOCUS       sequence-190412-        9655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GNAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: GNAS_pLX307
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2506..2530)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2538..2579
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2690..3278
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2743..2763)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3161..3172)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3350..3583
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3661..3782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3698..3717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3752..3771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3822..3957
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3977..3996)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3978..3996)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4006..4023)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4006..4022)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4015..4037)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4164..4619
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4251..4270)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4461..4482
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4645..4749
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4750..4818,4819..5610)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4968..4987)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5781..6369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6270..6289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6523..6540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6657..6678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6693..6698,6699..6716,6717..6723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6731..6747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6736..6758
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6755..6771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6755..6771
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6790..6810
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6792..6810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6838..7064
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7065..7245
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7292..7417
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7910..8143
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8328..8372
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8603..8813
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8625..8821
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8672..8807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8853..9455
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8853..8872)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9349..9369
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9498..9615
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgggctgcct cgggaacagt aagaccgagg accagcgcaa cgaggagaag gcgcagcgtg
     1381 aggccaacaa aaagatcgag aagcagctgc agaaggacaa gcaggtctac cgggccacgc
     1441 accgcctgct gctgctgggt gctggagaat ctggtaaaag caccattgtg aagcagatga
     1501 ggatcctgca tgttaatggg tttaatggag agggcggcga agaggacccg caggctgcaa
     1561 ggagcaacag cgatggcagt gagaaggcaa ccaaagtgca ggacatcaaa aacaacctga
     1621 aagaggcgat tgaaaccatt gtggccgcca tgagcaacct ggtgcccccc gtggagctgg
     1681 ccaaccccga gaaccagttc agagtggact acatcctgag tgtgatgaac gtgcctgact
     1741 ttgacttccc tcccgaattc tatgagcatg ccaaggctct gtgggaggat gaaggagtgc
     1801 gtgcctgcta cgaacgctcc aacgagtacc agctgattga ctgtgcccag tacttcctgg
     1861 acaagatcga cgtgatcaag caggctgact atgtgccgag cgatcaggac ctgcttcgct
     1921 gccgtgtcct gacttctgga atctttgaga ccaagttcca ggtggacaaa gtcaacttcc
     1981 acatgtttga cgtgggtggc cagcgcgatg aacgccgcaa gtggatccag tgcttcaacg
     2041 atgtgactgc catcatcttc gtggtggcca gcagcagcta caacatggtc atccgggagg
     2101 acaaccagac caaccgcctg caggaggctc tgaacctctt caagagcatc tggaacaaca
     2161 gatggctgcg caccatctct gtgatcctgt tcctcaacaa gcaagatctg ctcgctgaga
     2221 aagtccttgc tgggaaatcg aagattgagg actactttcc agaatttgct cgctacacta
     2281 ctcctgagga tgctactccc gagcccggag aggacccacg cgtgacccgg gccaagtact
     2341 tcattcgaga tgagtttctg aggatcagca ctgccagtgg agatgggcgt cactactgct
     2401 accctcattt cacctgcgct gtggacactg agaacatccg ccgtgtgttc aacgactgcc
     2461 gtgacatcat tcagcgcatg caccttcgtc agtacgagct gctctaccca actttcttgt
     2521 acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
     2581 agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     2641 gaaaggacga gccgcacgac tctcaatgtt cattacgcgt taagtcgaca atcaacctct
     2701 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2761 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2821 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2881 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2941 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3001 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3061 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     3121 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3181 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     3241 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     3301 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     3361 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     3421 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3481 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3541 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca
     3601 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     3661 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3721 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3781 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     3841 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3901 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3961 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     4021 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4081 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4141 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4201 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4261 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4321 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4381 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4501 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4561 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     4621 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4681 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4741 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4801 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4861 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4921 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4981 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5041 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5101 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5161 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5221 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5281 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5341 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5401 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5461 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5521 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5581 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5641 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5701 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5761 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5821 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5881 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5941 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6001 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6061 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6121 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6181 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6241 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6301 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6361 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6421 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6481 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6541 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6601 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6661 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6721 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6781 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     6841 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     6901 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     6961 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     7021 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     7081 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7141 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7201 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     7261 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     7321 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     7381 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     7441 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     7501 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     7561 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     7621 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     7681 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     7741 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     7801 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     7861 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     7921 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     7981 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     8041 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     8101 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     8161 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     8221 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     8281 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     8341 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     8401 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     8461 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     8521 cccacctccc aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg
     8581 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc
     8641 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8701 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8761 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8821 aaagctttct agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
     8881 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     8941 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     9001 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     9061 gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     9121 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     9181 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     9241 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     9301 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     9361 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     9421 cctggtgcat gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg
     9481 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     9541 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     9601 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgcc
//
