LOCUS       sequence-190414-       10144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HNF1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: HNF1B_pLX307
FEATURES             Location/Qualifiers
     source          1..10144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2995..3019)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3027..3068
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3179..3767
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3232..3252)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3650..3661)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3839..4072
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4150..4271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4187..4206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4241..4260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4311..4446
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4466..4485)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4467..4485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4495..4512)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4495..4511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4504..4526)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4653..5108
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4740..4759)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4950..4971
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5134..5238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5239..5307,5308..6099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5457..5476)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6270..6858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6759..6778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7012..7029
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7146..7167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7182..7187,7188..7205,7206..7212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7220..7236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7225..7247
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7244..7260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7244..7260
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7279..7299
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7281..7299
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7327..7553
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7554..7734
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7781..7906
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8399..8632
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8817..8861
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9092..9302
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9114..9310
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9161..9296
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9342..9944
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9342..9361)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9838..9858
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9987..10104
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggtgtccaa gctcacgtcg ctccagcaag aactcctgag cgccctgctg agctccgggg
     1381 tcaccaagga ggtgctggtt caggccttgg aggagttgct gccatccccg aacttcgggg
     1441 tgaagctgga gacgctgccc ctgtcccctg gcagcggggc cgagcccgac accaagccgg
     1501 tcttccatac tctcaccaac ggccacgcca agggccgctt gtccggcgac gagggctccg
     1561 aggacggcga cgactatgac acacctccca tcctcaagga gctgcaggcg ctcaacaccg
     1621 aggaggcggc ggagcagcgg gcggaggtgg accggatgct cagtgaggac ccttggaggg
     1681 ctgctaaaat gatcaagggt tacatgcagc aacacaacat cccccagagg gaggtggtcg
     1741 atgtcaccgg cctgaaccag tcgcacctct cccagcatct caacaagggc acccctatga
     1801 agacccagaa gcgtgccgct ctgtacacct ggtacgtcag aaagcaacga gagatcctcc
     1861 gacaattcaa ccagacagtc cagagttctg gaaatatgac agacaaaagc agtcaggatc
     1921 agctgctgtt tctctttcca gagttcagtc aacagagcca tgggcctggg cagtccgatg
     1981 atgcctgctc tgagcccacc aacaagaaga tgcgccgcaa ccggttcaaa tgggggcccg
     2041 cgtcccagca aatcttgtac caggcctacg atcggcaaaa gaaccccagc aaggaagaga
     2101 gagaggcctt agtggaggaa tgcaacaggg cagaatgttt gcagcgaggg gtgtccccct
     2161 ccaaagccca cggcctgggc tccaacttgg tcactgaggt ccgtgtctac aactggtttg
     2221 caaaccgcag gaaggaggag gcattccggc aaaagctggc catggacgcc tatagctcca
     2281 accagactca cagcctgaac cctctgctct cccacggctc cccccaccac cagcccagct
     2341 cctctcctcc aaacaagctg tcaggagtgc gctacagcca gcagggaaac aatgagatca
     2401 cttcctcctc aacaatcagt caccatggca acagcgccat ggtgaccagc cagtcggttt
     2461 tacagcaagt ctccccagcc agcctggacc caggccacaa tctcctctca cctgatggta
     2521 aaatgatctc agtctcagga ggaggtttgc ccccagtcag caccttgacg aatatccaca
     2581 gcctctccca ccataatccc cagcaatctc aaaacctcat catgacaccc ctctctggag
     2641 tcatggcaat tgcacaaagc ctcaacacct cccaagcaca gagtgtccct gtcatcaaca
     2701 gtgtggccgg cagcctggca gccctgcagc ccgtccagtt ctcccagcag ctgcacagcc
     2761 ctcaccagca gcccctcatg cagcagagcc caggcagcca catggcccag cagcccttca
     2821 tggcagctgt gactcagctg cagaactcac acatgtacgc acacaagcag gaaccccccc
     2881 agtattccca cacctcccgg tttccatctg caatggtggt cacagatacc agcagcatca
     2941 gtacactcac caacatgtct tcaagtaaac agtgtcctct acaagcctgg gaacccccaa
     3001 ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg
     3061 attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata
     3121 tatcttgtgg aaaggacgat actaaaaccc ttcctcggaa ccgacgcgtt aagtcgacaa
     3181 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3241 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3301 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3361 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3421 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3481 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3541 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3601 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3661 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3721 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     3781 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     3841 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     3901 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3961 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4021 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     4081 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     4141 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4201 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4261 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     4321 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4381 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4441 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4501 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4561 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4621 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4681 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4741 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4801 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4861 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4921 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4981 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5041 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5101 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5161 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5221 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5281 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5341 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5401 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5461 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5521 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5581 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5641 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5701 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5761 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5821 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5881 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5941 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6001 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6061 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6121 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6181 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6241 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6301 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6361 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6421 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6481 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6541 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6601 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6661 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6721 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6781 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6841 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6901 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6961 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7021 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7081 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7141 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7201 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7261 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7321 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7381 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7441 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7501 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7561 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7621 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7681 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7741 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7801 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7861 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     7921 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     7981 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8041 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8101 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8161 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8221 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8281 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8341 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8401 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8461 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8521 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8581 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8641 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8701 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8761 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8821 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8881 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8941 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9001 cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc
     9061 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct
     9121 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9181 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9241 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9301 cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca
     9361 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     9421 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     9481 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     9541 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     9601 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     9661 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     9721 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     9781 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     9841 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     9901 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg
     9961 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    10021 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    10081 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
    10141 cgcc
//
