LOCUS       sequence-190415-       12571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IGF1R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 12571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: IGF1R_pLX307
FEATURES             Location/Qualifiers
     source          1..12571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5422..5446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5454..5495
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5606..6194
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5659..5679)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6077..6088)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6266..6499
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6577..6698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6614..6633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6668..6687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6738..6873
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6893..6912)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6894..6912)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6922..6939)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6922..6938)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6931..6953)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7080..7535
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7167..7186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7377..7398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7561..7665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7666..7734,7735..8526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7884..7903)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8697..9285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9186..9205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9439..9456
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9573..9594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9609..9614,9615..9632,9633..9639)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9647..9663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9652..9674
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9671..9687
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9671..9687
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9706..9726
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9708..9726
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9754..9980
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9981..10161
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10208..10333
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10826..11059
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11244..11288
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11519..11729
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11541..11737
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11588..11723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11769..12371
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11769..11788)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12265..12285
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12414..12531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 ccatgaagtc tggctccgga ggagggtccc cgacctcgct gtgggggctc ctgtttctct
     1381 ccgccgcgct ctcgctctgg ccgacgagtg gagaaatctg cgggccaggc atcgacatcc
     1441 gcaacgacta tcagcagctg aagcgcctgg agaactgcac ggtgatcgag ggctacctcc
     1501 acatcctgct catctccaag gccgaggact accgcagcta ccgcttcccc aagctcacgg
     1561 tcattaccga gtacttgctg ctgttccgag tggctggcct cgagagcctc ggagacctct
     1621 tccccaacct cacggtcatc cgcggctgga aactcttcta caactacgcc ctggtcatct
     1681 tcgagatgac caatctcaag gatattgggc tttacaacct gaggaacatt actcgggggg
     1741 ccatcaggat tgagaaaaat gctgacctct gttacctctc cactgtggac tggtccctga
     1801 tcctggatgc ggtgtccaat aactacattg tggggaataa gcccccaaag gaatgtgggg
     1861 acctgtgtcc agggaccatg gaggagaagc cgatgtgtga gaagaccacc atcaacaatg
     1921 agtacaacta ccgctgctgg accacaaacc gctgccagaa aatgtgccca agcacgtgtg
     1981 ggaagcgggc gtgcaccgag aacaatgagt gctgccaccc cgagtgcctg ggcagctgca
     2041 gcgcgcctga caacgacacg gcctgtgtag cttgccgcca ctactactat gccggtgtct
     2101 gtgtgcctgc ctgcccgccc aacacctaca ggtttgaggg ctggcgctgt gtggaccgtg
     2161 acttctgcgc caacatcctc agcgccgaga gcagcgactc cgaggggttt gtgatccacg
     2221 acggcgagtg catgcaggag tgcccctcgg gcttcatccg caacggcagc cagagcatgt
     2281 actgcatccc ttgtgaaggt ccttgcccga aggtctgtga ggaagaaaag aaaacaaaga
     2341 ccattgattc tgttacttct gctcagatgc tccaaggatg caccatcttc aagggcaatt
     2401 tgctcattaa catccgacgg gggaataaca ttgcttcaga gctggagaac ttcatggggc
     2461 tcatcgaggt ggtgacgggc tacgtgaaga tccgccattc tcatgccttg gtctccttgt
     2521 ccttcctaaa aaaccttcgc ctcatcctag gagaggagca gctagaaggg aattactcct
     2581 tctacgtcct cgacaaccag aacttgcagc aactgtggga ctgggaccac cgcaacctga
     2641 ccatcaaagc agggaaaatg tactttgctt tcaatcccaa attatgtgtt tccgaaattt
     2701 accgcatgga ggaagtgacg gggactaaag ggcgccaaag caaaggggac ataaacacca
     2761 ggaacaacgg ggagagagcc tcctgtgaaa gtgacgtcct gcatttcacc tccaccacca
     2821 cgtcgaagaa tcgcatcatc ataacctggc accggtaccg gccccctgac tacagggatc
     2881 tcatcagctt caccgtttac tacaaggaag caccctttaa gaatgtcaca gagtatgatg
     2941 ggcaggatgc ctgcggctcc aacagctgga acatggtgga cgtggacctc ccgcccaaca
     3001 aggacgtgga gcccggcatc ttactacatg ggctgaagcc ctggactcag tacgccgttt
     3061 acgtcaaggc tgtgaccctc accatggtgg agaacgacca tatccgtggg gccaagagtg
     3121 agatcttgta cattcgcacc aatgcttcag ttccttccat tcccttggac gttctttcag
     3181 catcgaactc ctcttctcag ttaatcgtga agtggaaccc tccctctctg cccaacggca
     3241 acctgagtta ctacattgtg cgctggcagc ggcagcctca ggacggctac ctttaccggc
     3301 acaattactg ctccaaagac aaaatcccca tcaggaagta tgccgacggc accatcgaca
     3361 ttgaggaggt cacagagaac cccaagactg aggtgtgtgg tggggagaaa gggccttgct
     3421 gcgcctgccc caaaactgaa gccgagaagc aggccgagaa ggaggaggct gaataccgca
     3481 aagtctttga gaatttcctg cacaactcca tcttcgtgcc cagacctgaa aggaagcgga
     3541 gagatgtcat gcaagtggcc aacaccacca tgtccagccg aagcaggaac accacggccg
     3601 cagacaccta caacatcact gacccggaag agctggagac agagtaccct ttctttgaga
     3661 gcagagtgga taacaaggag agaactgtca tttctaacct tcggcctttc acattgtacc
     3721 gcatcgatat ccacagctgc aaccacgagg ctgagaagct gggctgcagc gcctccaact
     3781 tcgtctttgc aaggactatg cccgcagaag gagcagatga cattcctggg ccagtgacct
     3841 gggagccaag gcctgaaaac tccatctttt taaagtggcc ggaacctgag aatcccaatg
     3901 gattgattct aatgtatgaa ataaaatacg gatcacaagt tgaggatcag cgagaatgtg
     3961 tgtccagaca ggaatacagg aagtatggag gggccaagct aaaccggcta aacccgggga
     4021 actacacagc ccggattcag gccacatctc tctctgggaa tgggtcgtgg acagatcctg
     4081 tgttcttcta tgtccaggcc aaaacaggat atgaaaactt catccatctg atcatcgctc
     4141 tgcccgtcgc tgtcctgttg atcgtgggag ggttggtgat tatgctgtac gtcttccata
     4201 gaaagagaaa taacagcagg ctggggaatg gagtgctgta tgcctctgtg aacccggagt
     4261 acttcagcgc tgctgatgtg tacgttcctg atgagtggga ggtggctcgg gagaagatca
     4321 ccatgagccg ggaacttggg caggggtcgt ttgggatggt ctatgaagga gttgccaagg
     4381 gtgtggtgaa agatgaacct gaaaccagag tggccattaa aacagtgaac gaggccgcaa
     4441 gcatgcgtga gaggattgag tttctcaacg aagcttctgt gatgaaggag ttcaattgtc
     4501 accatgtggt gcgattgctg ggtgtggtgt cccaaggcca gccaacactg gtcatcatgg
     4561 aactgatgac acggggcgat ctcaaaagtt atctccggtc tctgaggcca gaaatggaga
     4621 ataatccagt cctagcacct ccaagcctga gcaagatgat tcagatggcc ggagagattg
     4681 cagacggcat ggcatacctc aacgccaata agttcgtcca cagagacctt gctgcccgga
     4741 attgcatggt agccgaagat ttcacagtca aaatcggaga ttttggtatg acgcgagata
     4801 tctatgagac agactattac cggaaaggag ggaaagggct gctgcccgtg cgctggatgt
     4861 ctcctgagtc cctcaaggat ggagtcttca ccacttactc ggacgtctgg tccttcgggg
     4921 tcgtcctctg ggagatcgcc acactggccg agcagcccta ccagggcttg tccaacgagc
     4981 aagtccttcg cttcgtcatg gagggcggcc ttctggacaa gccagacaac tgtcctgaca
     5041 tgctgtttga actgatgcgc atgtgctggc agtataaccc caagatgagg ccttccttcc
     5101 tggagatcat cagcagcatc aaagaggaga tggagcctgg cttccgggag gtctccttct
     5161 actacagcga ggagaacaag ctgcccgagc cggaggagct ggacctggag ccagagaaca
     5221 tggagagcgt ccccctggac ccctcggcct cctcgtcctc cctgccactg cccgacagac
     5281 actcaggaca caaggccgag aacggtcccg gccctggggt gctggtcctc cgcgccagct
     5341 tcgacgagag acagccttac gcccacatga acgggggccg caagaacgag cgggccttgc
     5401 cgctgcccca gtcttcgacc tgcccaactt tcttgtacaa agtggttgat atcggtaagc
     5461 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt ccgtaacttg
     5521 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaaa tcagcccata
     5581 cacagggttc acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     5641 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5701 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5761 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5821 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5881 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5941 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6001 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6061 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6121 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6181 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     6241 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6301 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6361 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6421 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6481 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     6541 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     6601 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6661 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     6721 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6781 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6841 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     6901 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6961 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7021 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     7081 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7141 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7201 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7261 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7321 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7381 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7441 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7501 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     7561 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7621 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7681 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7741 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7801 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7861 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7921 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7981 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8041 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8101 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8161 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8221 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8281 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8341 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8401 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8461 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8521 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8581 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8641 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8701 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8761 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8821 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8881 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8941 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9001 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9061 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9121 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9181 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9241 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9301 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9361 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9421 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     9481 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9541 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9601 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9661 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     9721 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     9781 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     9841 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     9901 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     9961 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
    10021 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10081 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10141 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    10201 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    10261 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    10321 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    10381 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    10441 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    10501 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    10561 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    10621 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
    10681 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    10741 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    10801 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    10861 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    10921 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    10981 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    11041 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    11101 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    11161 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    11221 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    11281 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    11341 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    11401 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    11461 ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    11521 aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac
    11581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    11641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    11701 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg
    11761 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
    11821 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
    11881 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
    11941 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
    12001 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
    12061 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
    12121 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
    12181 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
    12241 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
    12301 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
    12361 gtgccagtta acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa
    12421 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    12481 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    12541 tacagggaca gcagagatcc actttggcgc c
//
