LOCUS       sequence-190417-        8802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of UQCRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: UQCRB_pLX307
FEATURES             Location/Qualifiers
     source          1..8802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1654..1678)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1686..1727
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1838..2426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1891..1911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2309..2320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2498..2731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2809..2930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2846..2865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2900..2919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2970..3105
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3125..3144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3126..3144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3154..3171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3154..3170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3163..3185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3312..3767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3399..3418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3609..3630
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3793..3897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3898..3966,3967..4758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4116..4135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4929..5517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5418..5437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5671..5688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5805..5826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5841..5846,5847..5864,5865..5871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5879..5895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5884..5906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5903..5919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5903..5919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5938..5958
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5940..5958
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5986..6212
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6213..6393
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6440..6565
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7058..7291
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        7750..7960
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7772..7968
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7819..7954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8000..8602
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8000..8019)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8496..8516
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8645..8762
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggctggtaa gcaggccgtt tcagcatcag gcaagtggct ggatggtatt cgaaaatggt
     1381 attacaatgc tgcaggattc aataaactgg ggttaatgcg agatgataca atatacgagg
     1441 atgaagatgt aaaagaagcc ataagaagac ttcctgagaa cctttataat gacaggatgt
     1501 ttcgcattaa gagggcactg gacctgaact tgaagcatca gatcttgcct aaagagcagt
     1561 ggaccaaata tgaagaggaa aatttctacc ttgaaccgta tctgaaagag gttattcggg
     1621 aaagaaaaga aagagaagaa tgggcaaaga agtacccaac tttcttgtac aaagtggttg
     1681 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta
     1741 gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaac
     1801 cctaggacta gctccccaaa ccacgcgtta agtcgacaat caacctctgg attacaaaat
     1861 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     1921 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     1981 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     2041 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     2101 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     2161 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     2221 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     2281 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     2341 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     2401 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     2461 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     2521 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     2581 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     2641 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     2701 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     2761 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     2821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     2941 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3001 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3061 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     3121 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     3181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3301 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3361 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3421 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3481 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3541 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3601 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3661 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3721 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     3781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3901 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3961 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4021 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4081 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4141 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4201 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4261 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4321 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4381 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4441 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4501 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4561 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4621 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4681 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4741 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5041 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     5641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     5701 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     5761 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     5821 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     5881 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     5941 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     6001 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     6061 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     6121 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     6181 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     6241 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6301 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6361 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     6421 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     6481 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     6541 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     6601 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     6661 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     6721 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     6781 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     6841 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     6901 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     6961 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     7021 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     7081 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     7141 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     7201 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     7261 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     7321 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     7381 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     7441 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     7501 tagataaatg ggaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     7561 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     7621 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     7681 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7741 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     7801 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7861 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7921 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     7981 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8041 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8101 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8161 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     8221 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8281 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8341 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8401 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8461 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8521 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8581 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     8641 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8701 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     8761 ttcgggttta ttacagggac agcagagatc cactttggcg cc
//
