LOCUS       sequence-190420-       10768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of TLK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: TLK1_pLX307
FEATURES             Location/Qualifiers
     source          1..10768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3619..3643)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3651..3692
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3803..4391
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3856..3876)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4274..4285)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4463..4696
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4774..4895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4811..4830)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4865..4884
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4935..5070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5090..5109)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5091..5109)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5119..5136)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5119..5135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5128..5150)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5277..5732
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5364..5383)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5574..5595
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5758..5862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5863..5931,5932..6723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6081..6100)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6894..7482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7383..7402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7636..7653
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7770..7791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7806..7811,7812..7829,7830..7836)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7844..7860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7849..7871
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7868..7884
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7868..7884
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7903..7923
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7905..7923
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7951..8177
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8178..8358
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8405..8530
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9023..9256
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9441..9485
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9716..9926
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9738..9934
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9785..9920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9966..10568
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9966..9985)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10462..10482
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10611..10728
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgagtgtcca aagtagcagt ggaagtttgg aggggccgcc atcttggtcc cagctctcca
     1381 cgtctccaac cccgggctcg gcggcggcgg ccaggtccct gctgaatcac acgccgccat
     1441 ccgggaggcc cagggaaggt gcaatggatg agcttcatag tctggatcca agaaggcaag
     1501 agttattgga agctagattt actggagttg caagtgggag cactggaagt acgggcagtt
     1561 gcagtgttgg agctaaagcc tcaacaaata acgaaagctc taatcacagt tttggaagct
     1621 tgggatcttt aagtgacaaa gaatcagaga caccggagaa gaaacaatcg gaatcatcca
     1681 ggggaagaaa gagaaaagca gaaaaccaga atgaaagtag tcagggaaaa agtattgggg
     1741 gacgtggcca caaaattagc gactattttg aataccaggg tggaaatggc tcaagtccag
     1801 taagaggcat acctcctgca atccgttctc ctcaaaattc acattcacat tccactcctt
     1861 cctcatctgt tcgaccgaat agcccttctc ctactgcatt agcatttggg gaccacccta
     1921 ttgtacaacc aaagcaatta tcctttaaaa ttattcagac tgatctcaca atgctgaaat
     1981 tagcagcatt agaaagtaat aaaatccagg acctggaaaa gaaggaggga cgtatagatg
     2041 atttgctcag ggctaactgt gatctcagac ggcaaataga tgaacaacaa aaattacttg
     2101 aaaaatacaa agaacgatta aataagtgca tatcaatgag caagaaactt cttattgaaa
     2161 agagtacaca agaaaagctg tcaagcagag agaagagtat gcaagatcga ttacgccttg
     2221 ggcactttac aacagttaga catggcgctt catttactga acaatggaca gatggttttg
     2281 catttcagaa tcttgtgaag caacaagaat gggtgaatca gcaaagggaa gatattgaaa
     2341 ggcaaaggaa acttctagcc aaacgcaaac ctcccacagc taataattct caggcaccct
     2401 ctaccaattc tgaaccaaaa caaaggaaaa acaaagcagt caatggagca gagaatgatc
     2461 cctttgttag accaaattta ccacaactgt tgactttggc agaatatcat gaacaggaag
     2521 aaattttcaa acttagacta ggacatctca aaaaggaaga ggcagaaatc caggcagaac
     2581 ttgaacgttt ggaaagagtc agaaatcttc acatacgtga gctgaaaaga ataaacaatg
     2641 aagataattc acagttcaaa gatcacccaa cattaaatga aagatattta ttacttcatc
     2701 tgcttggtag aggtggcttt agtgaagtgt ataaggcttt tgacctttat gaacaaagat
     2761 atgctgctgt gaagatacat cagcttaata aaagctggag agatgagaag aaagaaaact
     2821 accacaaaca tgcctgcaga gagtatagaa tacacaaaga actggatcac cccagaatag
     2881 ttaaactcta tgattatttc tccttggata cagatacgtt ttgtacagtg ttagaatact
     2941 gtgaaggcaa tgacttggat ttctatctga agcaacacaa gttaatgtca gagaaagaag
     3001 ctcggtctat tgtaatgcag attgtaaatg cactaagata tctcaatgag atcaaacccc
     3061 ctattataca ttatgatctt aagccaggaa acatcctact ggtagatgga acagcatgtg
     3121 gtgaaatcaa aatcactgat tttggtctgt ccaagattat ggatgatgat agctatggtg
     3181 tagatggaat ggatctaact tcccaggggg caggcactta ctggtattta cctcctgagt
     3241 gttttgtagt tggaaaagag ccaccaaaga tttccaacaa ggttgatgta tggtcggttg
     3301 gagtcatctt ctttcagtgt ctttatggta gaaagccatt tggtcacaat caatctcaac
     3361 aagacattct tcaagaaaat acaatattaa aagccacaga agtccagttc cctgtaaaac
     3421 cggttgtaag cagtgaagcc aaggcattta taagacgctg tttggcatat cgaaaagaag
     3481 atcgatttga tgtgcaccag ctggcaaatg acccatacct tctcccacac atgagaagat
     3541 caaattcttc aggaaaccta cacatggctg ggctgacagc atcccctaca cccccttctt
     3601 caagcataat tacttactgc ccaactttct tgtacaaagt ggttgatatc ggtaagccta
     3661 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg taacttgaaa
     3721 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaacataag tttacccgag
     3781 aacgcggacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     3841 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3901 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3961 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4021 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4081 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4141 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     4201 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4261 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4321 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4381 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4441 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     4501 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4561 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4621 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4681 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     4741 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     4801 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4861 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     4921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5041 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     5101 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5161 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5221 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     5281 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5341 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5401 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5461 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5521 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5581 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5641 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5701 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     5761 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5821 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5881 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5941 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6001 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6061 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6121 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6181 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6241 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6301 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6361 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6421 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6481 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6541 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6601 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6661 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6721 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6781 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6841 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6901 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6961 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7021 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7081 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7141 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7201 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7261 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7321 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7381 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7441 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7501 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7561 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7621 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7681 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7741 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7801 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7861 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     7921 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     7981 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     8041 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     8101 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     8161 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8221 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8281 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8341 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     8401 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     8461 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     8521 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     8581 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     8641 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     8701 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     8761 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     8821 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     8881 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8941 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     9001 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     9061 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     9121 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     9181 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     9241 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     9301 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     9361 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     9421 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     9481 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     9541 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     9601 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca
     9661 tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9721 cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc
     9781 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9841 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9901 aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc
     9961 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
    10021 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
    10081 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
    10141 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
    10201 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
    10261 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
    10321 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
    10381 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
    10441 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
    10501 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
    10561 ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
    10621 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
    10681 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
    10741 agggacagca gagatccact ttggcgcc
//
