LOCUS       sequence-190424-       12616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of TRAPPC8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12616)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 12616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: TRAPPC8_pLX307
FEATURES             Location/Qualifiers
     source          1..12616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5467..5491)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5499..5540
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5651..6239
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5704..5724)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6122..6133)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6311..6544
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6622..6743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6659..6678)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6713..6732
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6783..6918
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6938..6957)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6939..6957)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6967..6984)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6967..6983)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6976..6998)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7125..7580
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7212..7231)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7422..7443
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7606..7710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7711..7779,7780..8571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7929..7948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8742..9330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9231..9250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9484..9501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9618..9639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9654..9659,9660..9677,9678..9684)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9692..9708
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9697..9719
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9716..9732
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9716..9732
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9751..9771
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9753..9771
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9799..10025
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10026..10206
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10253..10378
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10871..11104
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11289..11333
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11564..11774
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11586..11782
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11633..11768
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11814..12416
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11814..11833)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12310..12330
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12459..12576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 ccatgagaga tcctaataat caacttcacg taattaaaaa tttgaagata gcagtaagca
     1381 acattgtcac ccagccacct cagcctggag ccatccggaa gcttttgaat gatgttgttt
     1441 ctggcagtca gcctgcagaa ggattagtag ctaatgtgat tacagcagga gattatgacc
     1501 ttaacatcag tgccactact ccatggtttg agtcttacag agaaaccttt cttcagtcga
     1561 tgccagcatc ggatcatgaa tttctgaacc actatttagc atgtatgttg gtagcgtcat
     1621 ctagtgaagc tgaacctgtg gaacagtttt caaagttgtc acaagaacag catcgaattc
     1681 agcacaacag tgattattcc taccccaagt ggtttatacc aaatacactt aaatactatg
     1741 tacttttaca tgatgtaagt gcaggagatg aacagagagc tgaatcaatt tatgaagaaa
     1801 tgaaacagaa atatggaact cagggttgct atttacttaa aattaattct cgaacatcta
     1861 atcgagcatc agatgaacag ataccagatc cttggagtca gtatctccag aaaaatagta
     1921 ttcaaaacca ggaatcatat gaagatggcc cttgtactat aacttcaaat aagaattctg
     1981 ataataactt gctttcattg gatggattag ataacgaagt caaagatggc ttaccaaata
     2041 actttagagc tcacccactt cagttggagc aatccagtga cccttctaac agtattgatg
     2101 gcccagatca tctaagatct gcttcatcgt tacatgaaac aaagaaagga aatactggaa
     2161 taattcatgg tgcatgttta acacttactg atcatgatag aattcgacag tttatacaag
     2221 agttcacatt tcggggcctt ttgccacata tagagaaaac aattaggcaa ttaaacgatc
     2281 agctaatatc aagaaaaggt ttgagtcgat ctctattttc tgcaactaaa aaatggttta
     2341 gtggcagtaa agttccagaa aagagcatta atgacctgaa aaatacatct ggcttgctgt
     2401 atccgccgga agcaccagaa cttcaaatca ggaaaatggc tgacttatgt tttttggtgc
     2461 agcattatga tttggcttac agttgctatc atactgcaaa gaaagatttt cttaatgatc
     2521 aagcaatgct ttatgcagct ggtgccttgg aaatggcagc agtgtctgct tttcttcaac
     2581 caggagcacc taggccatat cctgctcatt acatggatac agcaattcag acatacagag
     2641 atatctgcaa gaatatggtg ttggctgaaa gatgtgtgtt gcttagtgct gaacttttaa
     2701 aaagccaaag caaatattca gaggctgcag ctctcctaat acggttgacc agtgaggatt
     2761 ctgatcttcg aagtgcactt cttttggaac aggcagcaca ttgctttata aacatgaaaa
     2821 gtcccatggt tagaaaatat gcatttcata tgatattggc aggccatcga tttagtaaag
     2881 cagggcagaa aaagcatgct ttacgctgtt attgtcaagc catgcaagtt tacaaaggaa
     2941 aaggctggtc tcttgcagag gatcacatta atttcactat tgggcgccag tcctatactc
     3001 ttagacagct ggataatgct gtgtctgctt ttaggcatat tctaattaat gaaagtaaac
     3061 aatctgctgc tcaacagggg gctttcctca gagaatatct ttatgtttac aagaatgtaa
     3121 gtcagctgtc accagatggt cctttgccac agcttccttt accgtatatt aacagttcag
     3181 caacacgggt tttttttggc catgacagac gaccagcgga tggtgaaaaa caagcagcta
     3241 ctcatgtaag tcttgatcaa gaatatgatt ctgaatcctc tcagcagtgg cgagaacttg
     3301 aggaacaagt tgtttctgtg gttaacaaag gagtaattcc atccaatttt catcccacac
     3361 aatactgttt gaacagttac tcagataatt caagatttcc acttgcagtt gtagaagaac
     3421 caattacagt ggaagtggct tttagaaacc ctttgaaagt tctacttttg ttgactgatt
     3481 tgtcattgct ttggaagttt catcctaaag atttcagtgg aaaggataat gaagaagtta
     3541 aacaactagt tacaagtgaa cctgaaatga ttggagctga agttatttca gagttcttaa
     3601 ttaatggcga agaatcaaaa gtggcaagac taaagctctt tccccatcac ataggggagc
     3661 tgcatattct gggagttgtt tataatcttg gcactattca gggctctatg acagtagatg
     3721 gcattggtgc tcttcccgga tgtcacacag gaaaatattc cttgagtatg tcagtccgag
     3781 ggaagcagga tttagaaatt caaggtcctc gacttaacaa cacaaaagaa gagaaaacat
     3841 ctgttaaata tggccctgat cgacgtttag atcccataat cacagaagaa atgccactgt
     3901 tggaggtgtt ctttatacat tttcctacag ggcttctctg tggagaaatc cgaaaagcat
     3961 atgtagaatt tgtcaatgtc agcaaatgtc cacttactgg attgaaggtt gtttctaaac
     4021 gtccagagtt ctttactttc ggtggtaata ctgctgttct aacaccacta agtccctcag
     4081 cttctgagaa ttgtagtgct tacaagactg ttgtgacaga tgctacctct gtgtgtacag
     4141 cactcatatc atcagcttct tctgtagact ttggcattgg cacaggaagt caaccagagg
     4201 tgattcctgt tccccttcct gacactgttc ttctacccgg agcctcagtg cagctgccaa
     4261 tgtggttacg tgggcctgat gaagaaggtg tccatgaaat taactttttg ttttactatg
     4321 aaagtgtcaa aaagcagcca aaaatacggc acagaatatt aagacacact gcaattattt
     4381 gtaccagtcg gtctttaaat gtacgggcca ctgtctgcag aagtaattct cttgaaaatg
     4441 aagaaggcag aggaggcaat atgctagtct ttgtggatgt ggaaaatacc aatactagtg
     4501 aagcaggcgt taaggaattc cacatagtgc aagtatcaag tagtagcaaa cactggaagt
     4561 tacagaaatc tgtaaatctt tctgaaaaca aagataccaa acttgccagt agggagaagg
     4621 gaaagttttg ctttaaggca ataagatgtg agaaagaaga agcggccaca cagtcctctg
     4681 aaaaatatac ctttgcagat atcatctttg gaaatgaaca gataataagt tcagcaagcc
     4741 catgtgcaga cttcttttat cgaagtttat cttctgaatt gaaaaaacca caagctcact
     4801 tgcctgtgca tacagaaaaa cagtcaacag aggatgctgt gagattgatt caaaaatgca
     4861 gtgaggtaga tttgaatatt gtcatattat ggaaggcata cgttgtggaa gacagtaaac
     4921 agcttatttt ggaaggtcaa catcatgtta ttcttcgcac tataggaaaa gaagcctttt
     4981 catatcctca gaaacaggag ccaccagaaa tggaactatt gaaatttttc aggccagaaa
     5041 acattacagt ttcctcaagg ccatcagtag agcagctttc tagtctcatt aaaacgagtc
     5101 ttcactaccc agaatcattt aatcatccat ttcatcaaaa aagcctttgt ttagtaccag
     5161 tcactctttt actttccaat tgttctaagg ctgatgtaga tgtcatagtt gatcttcggc
     5221 ataaaacaac aagtccagaa gcactggaaa tccatggatc attcacatgg cttggacaaa
     5281 cacagtataa acttcaactt aaaagccagg agattcacag tctgcagctg aaagcatgct
     5341 ttgttcatac aggtgtttat aaccttggaa ctcctagggt atttgccaag ttatcggacc
     5401 aagttacagt gtttgaaaca agtcagcaga attccatgcc tgccctgatc atcatcagta
     5461 atgtgttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc
     5521 tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt atttcgattt
     5581 cttggcttta tatatcttgt ggaaaggacg attgctatcc ggcctctaaa atgggacgcg
     5641 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5701 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5761 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5821 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5881 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5941 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6001 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6061 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6121 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6181 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     6241 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6301 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6361 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6421 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6481 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6541 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6601 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     6661 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6721 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     6781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6841 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6901 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     6961 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7021 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7081 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     7141 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7201 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7261 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7321 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7381 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7441 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     7501 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     7561 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7621 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7681 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7741 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7801 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7861 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7921 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7981 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8041 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8101 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8161 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8221 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8281 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8341 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8401 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8461 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8521 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8581 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8641 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8701 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8761 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8821 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8881 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8941 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9001 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9061 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9121 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9181 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9241 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9301 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9361 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9421 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9481 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     9541 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     9601 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     9661 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     9721 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     9781 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     9841 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     9901 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     9961 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
    10021 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    10081 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    10141 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    10201 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
    10261 acgcaggact cggcttgctg tagcgcgcac ggcaagaggc gaggggcggc gactggtgag
    10321 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
    10381 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
    10441 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
    10501 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
    10561 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
    10621 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
    10681 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
    10741 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    10801 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    10861 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    10921 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
    10981 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
    11041 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
    11101 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
    11161 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
    11221 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
    11281 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
    11341 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
    11401 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
    11461 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct
    11521 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    11581 aagcatgcgt ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    11641 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    11701 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    11761 tggaggccta ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg
    11821 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
    11881 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
    11941 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
    12001 aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg
    12061 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
    12121 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
    12181 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
    12241 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
    12301 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
    12361 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa
    12421 ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
    12481 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
    12541 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
    12601 gatccacttt ggcgcc
//
