LOCUS       sequence-190425-       10225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of TCF7L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: TCF7L2_pLX307
FEATURES             Location/Qualifiers
     source          1..10225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3076..3100)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3108..3149
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3260..3848
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3313..3333)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3731..3742)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3920..4153
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4231..4352
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4268..4287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4322..4341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4392..4527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4547..4566)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4548..4566)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4576..4593)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4576..4592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4585..4607)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4734..5189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4821..4840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5031..5052
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5215..5319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5320..5388,5389..6180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5538..5557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6351..6939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6840..6859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7093..7110
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7227..7248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7263..7268,7269..7286,7287..7293)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7301..7317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7306..7328
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7325..7341
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7325..7341
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7360..7380
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7362..7380
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7408..7634
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7635..7815
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7862..7987
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8480..8713
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8898..8942
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9173..9383
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9195..9391
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9242..9377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9423..10025
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9423..9442)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9919..9939
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10068..10185
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgccgcagct gaacggcggt ggaggggatg acctaggcgc caacgacgaa ctgatttcct
     1381 tcaaagacga gggcgaacag gaggagaaga gctccgaaaa ctcctcggca gagagggatt
     1441 tagctgatgt caaatcgtct ctagtcaatg aatcagaaac gaatcaaaac agctcctccg
     1501 attccgaggc ggaaagacgg cctccgcctc gctccgaaag tttccgagac aaatcccggg
     1561 aaagtttgga agaagcggcc aagaggcaag atggagggct ctttaagggg ccaccgtatc
     1621 ccggctaccc cttcatcatg atccccgacc tgacgagccc ctacctcccc aacggatcgc
     1681 tctcgcccac cgcccgaacc tatctccaga tgaaatggcc actgcttgat gtccaggcag
     1741 ggagcctcca gagtagacaa gccctcaagg atgcccggtc cccatcaccg gcacacattg
     1801 tctctaacaa agtgccagtg gtgcagcacc ctcaccatgt ccaccccctc acgcctctta
     1861 tcacgtacag caatgaacac ttcacgccgg gaaacccacc tccacactta ccagccgacg
     1921 tagaccccaa aacaggaatc ccacggcctc cgcaccctcc agatatatcc ccgtattacc
     1981 cactatcgcc tggcaccgta ggacaaatcc cccatccgct aggatggtta gtaccacagc
     2041 aaggtcaacc agtgtaccca atcacgacag gaggattcag acacccctac cccacagctc
     2101 tgaccgtcaa tgcttccatg tccagctttc tgtcttctag gttccctccc catatggtcc
     2161 caccacatca tacgctacac acgacgggca ttccgcatcc ggccatagtc acaccaacag
     2221 tcaaacagga atcgtcccag agtgatgtcg gctcactcca tagttcaaag catcaggact
     2281 ccaaaaagga agaagaaaag aagaagcccc acataaagaa acctcttaat gcattcatgt
     2341 tgtatatgaa ggaaatgaga gcaaaggtcg tagctgagtg cacgttgaaa gaaagcgcgg
     2401 ccatcaacca gatccttggg cggaggtggc atgcactgtc cagagaagag caagcgaaat
     2461 actacgagct ggcccggaag gagcgacagc ttcatatgca actgtacccc ggctggtccg
     2521 cgcgggataa ctatggaaag aagaagaaga ggaaaaggga caagcagccg ggagagacca
     2581 atgatgcaaa tactccaaag aagtgtcggg cactgttcgg gcttgaccga cagactttat
     2641 ggtgcaaacc gtgcaggaga aaaaaaaagt gcgttcgcta catacaaggt gaaggcagct
     2701 gcctcagccc accctcttca gatggaagct tactagattc gcctcccccc tccccgaacc
     2761 tgctaggctc ccctccccga gacgccaagt cacagactga gcagacccag cctctgtcgc
     2821 tgtccctgaa gcccgacccc ctggcccacc tgtccatgat gcctccgcca cccgccctcc
     2881 tgctcgctga ggccacccac aaggcctccg ccctctgtcc caacggggcc ctggacctgc
     2941 ccccagccgc tttgcagcct gccgccccct cctcatcaat tgcacagccg tcgacttctt
     3001 ccttacattc ccacagctcc ctggccggga cccagcccca gccgctgtcg ctcgtcacca
     3061 agtctttaga aagaccccca actttcttgt acaaagtggt tgatatcggt aagcctatcc
     3121 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa cttgaaagta
     3181 tttcgatttc ttggctttat atatcttgtg gaaaggacga tactaaaacc cttcctcgga
     3241 accgacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3301 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3361 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3421 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3481 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3541 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3601 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3661 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3721 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3781 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3841 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3901 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3961 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4021 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4081 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4141 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     4201 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4381 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4441 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4501 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4561 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4621 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4681 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4741 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4801 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4861 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4921 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4981 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5041 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5101 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5161 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5221 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5281 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5341 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5401 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5461 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5521 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5581 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5641 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5701 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5761 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5821 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5881 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5941 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6001 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6061 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6121 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6181 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6241 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6301 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6361 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6421 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6481 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6541 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6601 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6661 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6721 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6781 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6841 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6901 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6961 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7021 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7081 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7141 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7201 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7261 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7321 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7381 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7441 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7501 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7561 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7621 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7681 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7741 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7801 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7861 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7921 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7981 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8041 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8101 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8161 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8221 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8281 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8341 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8401 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8461 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8521 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8581 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8641 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8701 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8761 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8821 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8881 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8941 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     9001 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     9061 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc
     9121 attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9181 aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9241 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9301 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9361 aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta
     9421 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     9481 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     9541 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     9601 acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg
     9661 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     9721 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     9781 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     9841 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     9901 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     9961 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca
    10021 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
    10081 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    10141 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
    10201 gacagcagag atccactttg gcgcc
//
