LOCUS       sequence-190427-       11029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RBM10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: RBM10_pLX307
FEATURES             Location/Qualifiers
     source          1..11029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1826..1843)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(2439..2450)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3880..3904)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3912..3953
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4064..4652
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4117..4137)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4535..4546)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4724..4957
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5035..5156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5072..5091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5126..5145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5196..5331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5351..5370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5352..5370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5380..5397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5380..5396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5389..5411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5538..5993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5625..5644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5835..5856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6019..6123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6124..6192,6193..6984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6342..6361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7155..7743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7644..7663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7897..7914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8031..8052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8067..8072,8073..8090,8091..8097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8105..8121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8110..8132
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8129..8145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8129..8145
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8164..8184
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8166..8184
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8212..8438
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8439..8619
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8666..8791
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9284..9517
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9702..9746
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9977..10187
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9999..10195
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10046..10181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10227..10829
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10227..10246)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10723..10743
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10872..10989
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggagtatga aagacgtggt ggtcgtggtg acaggactgg ccgctatgga gccactgacc
     1381 gctcgcagga tgatggtggg gagaaccgca gccgagacca cgactaccgg gacatggact
     1441 accgttcata tcctcgcgag tatggcagcc aggagggcaa gcatgactat gacgactcat
     1501 ctgaggagca gagtgcggag atccgtggcc agctgcagtc gcacggcgtg caagcacggg
     1561 aggttcggct gatgcggaac aaatcttcag gtcagagccg gggcttcgcc ttcgtcgagt
     1621 ttagtcactt gcaggacgct acacgatgga tggaagccaa tcagcactcc ctcaacatcc
     1681 tgggccagaa ggtgtcgatg cactacagtg accccaagcc caagatcaat gaggactggc
     1741 tgtgcaataa gtgtggcgtc cagaacttca aacgccgaga gaagtgcttc aaatgtggcg
     1801 tgcccaagtc agaggcagag cagaagctgc ccctcggcac gaggctggat cagcagacac
     1861 tgccactggg tggccgggag ctgagccagg gcctgcttcc cctgccgcag ccctaccagg
     1921 cccagggagt cctggcctcc caagccctgt cacagggctc ggagccaagc tcagagaacg
     1981 ccaatgacac catcattttg cgcaacctga acccacacag caccatggat tccatcctgg
     2041 gggccctggc accctacgcg gtgctgtcct cctccaacgt gcgcgtcata aaggacaagc
     2101 agacccaact gaaccgcggc tttgccttca tccagctctc caccatcgag gcagcccagc
     2161 tgctgcagat cctgcaggcc ctgcacccac cactcactat cgacggcaag accatcaatg
     2221 ttgagtttgc caagggttct aagagggaca tggcctccaa tgaaggcagt cgcatcagtg
     2281 ctgcctctgt ggccagcact gccattgctg cggcccagtg ggccatctca caggcctccc
     2341 aaggtgggga gggtacctgg gccacctccg aggagccgcc ggtcgactac agctactacc
     2401 aacaggatga gggctatggc aacagccagg gcacagagtc ttccctctat gcccatggct
     2461 acctcaaggg caccaagggc cctggcatca ctggaaccaa aggggatccc actggagcag
     2521 gtcccgaggc ctccctagag cctggggccg actctgtgtc gatgcaggct ttctctcgcg
     2581 cccagcctgg tgctgctcct ggcatctacc aacaatcagc cgaggcgagc agtagccagg
     2641 gcactgctgc caacagccag tcgtatacca tcatgtcacc cgctgtgctc aaatctgagc
     2701 tccagagccc tacccatcct agttctgctc tcccaccggc taccagcccc actgcccagg
     2761 aatcctacag ccagtaccct gttcccgacg tctctaccta ccagtacgat gagacctccg
     2821 gctactacta tgacccccag accggcctct actatgaccc caactcccag tattactaca
     2881 atgctcagag ccagcagtac ctgtactggg atggggagag gcggacctat gttcccgccc
     2941 tggagcagtc ggccgacgga cataaggaga caggggcacc ctcgaaggag ggcaaagaga
     3001 agaaggagaa gcacaagacc aagacagctc aacagattgc caaggacatg gaacgctggg
     3061 cccgcagtct caacaaacaa aaagaaaact tcaaaaatag cttccagcct atcagctccc
     3121 tgcgagatga cgagaggcgg gagtcagcca ctgcagatgc tggctatgcc atcctcgaga
     3181 agaagggagc actagccgag agacagcaca ccagcatgga tctcccgaaa ttggccagtg
     3241 acgaccgccc aagccctccg cgaggactgg tggcagccta cagcggggag agtgacagtg
     3301 aggaggagca ggagcgtggg ggccctgagc gggaggagaa gctcaccgac tggcagaagc
     3361 tggcctgtct gctctgccga cgccagttcc ccagcaaaga ggcgctcatc cggcaccagc
     3421 agctctcagg gctccacaag caaaaccttg agattcaccg gcgagcccac ttgtcagaaa
     3481 acgagctaga agcactagag aagaatgaca tggagcaaat gaagtaccgg gaccgtgcag
     3541 ctgaacgcag agaaaagtat ggcatccccg agccgccaga gcccaagagg aggaagtacg
     3601 gcggcatatc cacagcctct gtagacttcg agcagcctac tcgggacggg ctgggcagtg
     3661 acaacattgg cagtcggatg ctgcaggcca tgggctggaa agagggcagc ggcctgggcc
     3721 gcaagaagca gggcattgta acgcctatcg aggcccaaac acgggtgcgg ggctccggcc
     3781 tgggtgcacg gggcagctcc tacggggtca cctcaaccga gtcctacaag gagacactgc
     3841 acaagacaat ggtgacccgc ttcaacgagg cccagagacc cccaactttc ttgtacaaag
     3901 tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     3961 gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     4021 acgatactaa aacccttcct cggaaccgac gcgttaagtc gacaatcaac ctctggatta
     4081 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4141 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4201 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4261 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4321 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4381 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4441 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     4501 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4561 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4621 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     4681 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4741 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4801 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4861 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4921 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     4981 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     5041 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5101 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5161 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5221 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5281 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     5341 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     5401 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5461 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5521 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5581 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5641 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5701 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5761 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5821 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5881 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5941 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     6001 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6061 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6121 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6181 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6241 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6301 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6361 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6421 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6481 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6541 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6601 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6661 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6721 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6781 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6841 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6901 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6961 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7021 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7261 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7801 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7861 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7921 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7981 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8041 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8101 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8161 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     8221 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     8281 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     8341 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     8401 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     8461 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8521 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8581 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     8641 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     8701 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     8761 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     8821 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     8881 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     8941 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     9001 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     9061 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     9121 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     9181 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     9241 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     9301 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     9361 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     9421 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     9481 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     9541 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     9601 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     9661 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     9721 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     9781 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     9841 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     9901 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
     9961 gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac
    10021 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    10081 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    10141 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    10201 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
    10261 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
    10321 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
    10381 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
    10441 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
    10501 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
    10561 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
    10621 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
    10681 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
    10741 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
    10801 gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta
    10861 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    10921 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
    10981 caaaattttc gggtttatta cagggacagc agagatccac tttggcgcc
//
