LOCUS       sequence-190428-        8929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RPS18.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 8929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: RPS18_pLX307
FEATURES             Location/Qualifiers
     source          1..8929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1780..1804)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1812..1853
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1964..2552
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2017..2037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2435..2446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2624..2857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2935..3056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2972..2991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3026..3045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3096..3231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3251..3270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3252..3270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3280..3297)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3280..3296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3289..3311)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3438..3893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3525..3544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3735..3756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3919..4023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4024..4092,4093..4884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4242..4261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5055..5643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5544..5563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5797..5814
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5931..5952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5967..5972,5973..5990,5991..5997)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6005..6021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6010..6032
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6029..6045
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6029..6045
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6064..6084
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6066..6084
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6112..6338
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6339..6519
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6566..6691
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7184..7417
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7602..7646
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7877..8087
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7899..8095
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7946..8081
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8127..8729
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8127..8146)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8623..8643
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8772..8889
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 ccatgtctct agtgatccct gaaaagttcc agcatatttt gcgagtactc aacaccaaca
     1381 tcgatgggcg gcggaaaata gcctttgcca tcactgccat taagggtgtg ggccgaagat
     1441 atgctcatgt ggtgttgagg aaagcagaca ttgacctcac caagagggcg ggagaactca
     1501 ctgaggatga ggtggaacgt gtgatcacca ttatgcagaa tccacgccag tacaagatcc
     1561 cagactggtt cttgaacaga cagaaggatg taaaggatgg aaaatacagc caggtcctag
     1621 ccaatggtct ggacaacaag ctccgtgaag acctggagcg actgaagaag attcgggccc
     1681 atagagggct gcgtcacttc tggggccttc gtgtccgagg ccagcacacc aagaccactg
     1741 gccgccgtgg ccgcaccgtg ggtgtgtcca agaagaaatt gccaactttc ttgtacaaag
     1801 tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     1861 gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     1921 acgaatggac gcggagaata gacaaccaac gcgttaagtc gacaatcaac ctctggatta
     1981 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2041 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2101 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2161 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2221 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2281 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2341 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     2401 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2461 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     2521 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     2581 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     2641 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     2701 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     2761 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     2821 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     2881 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     2941 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3001 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3061 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     3241 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     3301 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3361 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     3421 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3481 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3781 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3841 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     3901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3961 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4021 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4081 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4141 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4201 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4261 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4321 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4381 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4441 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4501 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4561 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4621 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4681 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4741 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4801 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4861 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4921 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4981 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5161 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5461 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     5761 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     5821 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     5881 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     5941 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6001 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6061 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     6121 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     6181 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     6241 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     6301 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     6361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6481 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     6541 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     6601 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     6661 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     6721 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     6781 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     6841 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     6901 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     6961 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     7021 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     7081 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7141 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7201 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     7261 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     7321 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     7381 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     7441 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     7501 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     7561 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     7621 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     7681 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     7741 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     7801 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
     7861 gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac
     7921 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7981 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8041 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8101 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     8161 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     8221 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     8281 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     8341 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     8401 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     8461 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     8521 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     8581 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     8641 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     8701 gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta
     8761 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     8821 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     8881 caaaattttc gggtttatta cagggacagc agagatccac tttggcgcc
//
