LOCUS       sequence-190430-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RNF138.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: RNF138_pLX307
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2056..2080)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2088..2129
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2240..2828
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2293..2313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2711..2722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2900..3133
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3211..3332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3248..3267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3302..3321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3372..3507
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3527..3546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3528..3546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3556..3573)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3556..3572)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3565..3587)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3714..4169
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3801..3820)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4011..4032
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4195..4299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4300..4368,4369..5160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4518..4537)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5331..5919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5820..5839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6073..6090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6207..6228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6243..6248,6249..6266,6267..6273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6281..6297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6286..6308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6305..6321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6305..6321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6340..6360
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6342..6360
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6388..6614
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6615..6795
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6842..6967
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7460..7693
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7878..7922
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8153..8363
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8175..8371
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8222..8357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8403..9005
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8403..8422)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8899..8919
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9048..9165
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggccgagga cctctctgcg gccacgtcct acaccgaaga tgatttctac tgccccgtct
     1381 gtcaggaggt gctcaaaacg cccgtgcgga ccacggcctg tcagcacgtt ttctgtagaa
     1441 aatgtttcct gactgcaatg agggaaagcg gagcacattg tcccctatgt cgtggaaatg
     1501 tgactagaag agagagagca tgtcctgaac gggccttaga ccttgaaaat ataatgagga
     1561 agttttctgg tagctgcaga tgctgtgcaa aacagattaa attctatcgc atgagacatc
     1621 attacaaatc ttgtaagaag tatcaggatg aatatggtgt ttcttctatc attccaaact
     1681 ttcagatctc tcaagattca gtagggaaca gcaataggag tgaaacatcc acatctgata
     1741 acacagaaac ttaccaagag aatacaagtt cttctggtca tcctactttt aagtgtcccc
     1801 tgtgtcaaga atcaaatttt accagacagc gtttactgga tcactgtaac agtaatcacc
     1861 tatttcagat agttcctgtg acatgtccta tttgtgtgtc tcttccttgg ggagatccta
     1921 gccagattac cagaaatttc gttagtcatc taaatcagag acatcaattt gattatggag
     1981 aatttgtgaa tcttcagcta gatgaagaaa cccaatacca aactgctgtt gaagaatctt
     2041 ttcaagtaaa catctgccca actttcttgt acaaagtggt tgatatcggt aagcctatcc
     2101 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa cttgaaagta
     2161 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacggcggtg ctaggcgggg
     2221 cccaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2281 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2341 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2401 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2461 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2521 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2581 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2641 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2701 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2761 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2821 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2881 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     2941 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3001 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3061 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3121 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3181 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3301 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3361 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3421 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3481 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3541 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3601 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3661 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3721 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3781 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3841 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3901 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3961 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4021 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4081 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4141 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4201 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4261 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4321 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4381 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4441 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4501 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4561 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4621 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4681 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4741 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4801 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4861 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4921 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4981 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5041 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5101 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5161 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5221 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5281 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5341 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5401 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5461 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5521 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5581 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5641 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5701 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5761 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5821 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5881 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5941 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6001 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6061 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6121 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6181 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6241 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6301 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6361 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     6421 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6481 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6541 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     6601 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6661 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6721 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6781 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6841 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6901 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6961 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7021 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7081 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7141 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7201 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7261 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7321 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7381 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7441 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7501 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7561 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7621 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7681 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7741 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7801 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7861 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     7921 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7981 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8041 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc
     8101 attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8161 aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     8221 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8281 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8341 aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta
     8401 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     8461 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     8521 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     8581 acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg
     8641 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     8701 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     8761 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     8821 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     8881 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     8941 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca
     9001 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     9061 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     9121 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     9181 gacagcagag atccactttg gcgcc
//
