LOCUS       sequence-190431-       10477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RBM14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: RBM14_pLX307
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3328..3352)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3360..3401
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3512..4100
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3565..3585)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3983..3994)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4172..4405
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4483..4604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4520..4539)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4574..4593
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4644..4779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4799..4818)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4800..4818)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4828..4845)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4828..4844)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4837..4859)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4986..5441
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5073..5092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5283..5304
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5467..5571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5572..5640,5641..6432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5790..5809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6603..7191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7092..7111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7345..7362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7479..7500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7515..7520,7521..7538,7539..7545)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7553..7569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7558..7580
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7577..7593
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7577..7593
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7612..7632
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7614..7632
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7660..7886
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7887..8067
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8114..8239
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8732..8965
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9150..9194
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9425..9635
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9447..9643
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9494..9629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9675..10277
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9675..9694)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10171..10191
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10320..10437
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgaagatatt cgtgggcaac gtcgacgggg cggatacgac tccggaggag ctggcagccc
     1381 tctttgcgcc ctacggcacg gtcatgagct gcgccgtcat gaaacagttc gccttcgtgc
     1441 acatgcgcga gaacgcgggc gcgctgcgcg ccatcgaagc cctgcacggc cacgagctgc
     1501 ggccggggcg cgcgctcgtg gtggagatgt cgcgcccaag gcctcttaat acttggaaga
     1561 ttttcgtggg caatgtgtcg gctgcatgca cgagccagga actgcgcagc ctcttcgagc
     1621 gccgcggacg cgtcatcgag tgtgacgtgg tgaaagacta cgcgtttgtt cacatggaga
     1681 aggaagcaga tgccaaagcc gcaatcgcgc agctcaacgg caaagaagtg aagggcaagc
     1741 gcatcaacgt ggaactctcc accaagggtc agaagaaggg gcctggcctg gctgtccagt
     1801 ctggggacaa gaccaagaaa ccaggggctg gggatacggc cttccctgga actggtggct
     1861 tctctgccac cttcgactac cagcaggctt ttggcaacag cactggtggc tttgatgggc
     1921 aagcccgtca gcccacacca cccttctttg gtcgcgaccg cagccctctg cgccgttcac
     1981 ctccccgagc ctcttatgtg gctcctctga cggcccagcc agctacctac cgggcccagc
     2041 cgtccgtgtc actgggagct gcctacaggg cccagccttc tgcctctttg ggtgttggct
     2101 atcggactca gcccatgaca gcccaggcag cctcttaccg cgctcagccc tctgtctccc
     2161 ttggggcacc atacaggggc cagctggcta gtcctagctc ccagtctgct gcagcttctt
     2221 cactcggccc atatggtgga gcccagccct cagcctcggc cctttcctcc tatgggggtc
     2281 aggcagctgc agcttcttcg ctcaactcct atggggctca gggttcctcc cttgcctcct
     2341 atggtaacca gccatcctct tacggcgccc aggctgcctc ttcctatggg gttcgtgcag
     2401 ctgcttcttc ctacaacacc cagggagcag cttcctcctt aggctcctac ggggctcagg
     2461 cagcctccta tggggcccag tctgcagcct cctcactagc ttatggagcc caggcagctt
     2521 catataatgc ccagccctcg gcctcttaca atgcccagtc tgccccatat gctgcacagc
     2581 aggctgcttc ctactcttcc caacctgctg cctatgtggc acagccagcc acagctgctg
     2641 cctatgccag ccagccagca gcctacgccg cacaagccac taccccaatg gctggctcct
     2701 atggggccca gccggttgtg cagacccagc tgaatagtta cggggcccaa gcatcaatgg
     2761 gcctttcagg ctcctatggg gctcagtcgg ctgctgcggc cactggctcc tatggtgccg
     2821 cagcagccta cggggcccaa ccttctgcca ccctggcagc tccttaccgc actcagtcat
     2881 cagcctcatt ggctgcttcc tatgctgccc agcagcatcc ccaggctgct gcctcctacc
     2941 gcggccagcc aggcaatgcc tacgatgggg caggtcagcc gtctgcagcc tacctgtcca
     3001 tgtcccaggg ggccgttgcc aacgccaaca gcaccccgcc gccctatgag cgtacccgcc
     3061 tctccccacc ccgggccagc tacgacgatc cctacaaaaa ggctgtcgcc atgtcgaaaa
     3121 ggtatggttc cgaccggcgt ttagccgagc tctctgatta ccgccgttta tcagagtcgc
     3181 agctttcgtt ccgccgctcg ccgacaaagt cctcgctgga ttaccgtcgc ctgcccgatg
     3241 cccattccga ttacgcacgc tattcgggct cctataatga ttacctgcgg gcggctcaga
     3301 tgcactctgg ctaccagcgc cgcatgtacc caactttctt gtacaaagtg gttgatatcg
     3361 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt
     3421 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gattacctgg
     3481 ggaatacaaa acaaccacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     3541 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3601 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3661 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3721 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3781 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3841 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3901 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3961 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4021 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4081 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     4141 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     4201 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     4261 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4321 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4381 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     4441 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     4501 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4561 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     4621 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4681 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4741 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     4801 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     4861 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4921 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4981 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5041 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5101 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5161 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5221 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5281 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5341 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5401 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     5461 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5521 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5581 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5641 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5701 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5761 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5821 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5881 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5941 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6001 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6061 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6121 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6181 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6241 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6301 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6361 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6421 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6541 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6601 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6661 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6721 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6781 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6841 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6901 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6961 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7021 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7081 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7141 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7201 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7261 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7321 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7441 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7501 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7561 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     7621 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     7681 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     7741 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     7801 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     7861 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     7921 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7981 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8041 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     8101 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     8161 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     8221 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     8281 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     8341 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     8401 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     8461 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     8521 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     8581 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     8641 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     8701 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     8761 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     8821 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     8881 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     8941 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     9001 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     9061 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     9121 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     9181 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     9241 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     9301 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     9361 ggggacccat gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     9421 cccagcaggc agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc
     9481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9601 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac
     9661 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     9721 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     9781 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     9841 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct
     9901 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     9961 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
    10021 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
    10081 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
    10141 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
    10201 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
    10261 agcccggtgc cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat
    10321 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    10381 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
    10441 gtttattaca gggacagcag agatccactt tggcgcc
//
